中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
28期
1967-1970
,共4页
殷德涛%陈国%李红强%郑湘予%王勇飞%张艳%江金花%王庆端
慇德濤%陳國%李紅彊%鄭湘予%王勇飛%張豔%江金花%王慶耑
은덕도%진국%리홍강%정상여%왕용비%장염%강금화%왕경단
甲状腺肿瘤%甲基化%免疫组织化学%X染色体%细胞凋亡
甲狀腺腫瘤%甲基化%免疫組織化學%X染色體%細胞凋亡
갑상선종류%갑기화%면역조직화학%X염색체%세포조망
Papillary thyroid neoplasms%Methylation%Immunohistochemistry%X-chromosome%Apoptosis
目的 探讨甲状腺乳头状癌(PTC)组织中X染色体连锁凋亡抑制因子相关因子1(XAF1)基因启动子甲基化及其与蛋白表达的相关性.方法 采用甲基化特异性聚合酶链反应(PCR,MSP)及免疫组化(SP法)检测70例PTC及其对应的癌旁非癌组织(NCE)中XAF1基因启动子甲基化及其蛋白的表达.结果 在NCE中,无一例出现XAF1基因启动子甲基化,而在PTC组织中有35.7％ (25/70)出现甲基化(x2=27.206,P＜0.01),且甲基化状态与PTC的TNM分期、病理分级及淋巴结转移有关(P＜0.05)；NCE和PTC中,XAF1蛋白表达的阳性率分别为100％ (70/70)和55.7％ (39/70)(x2=36.458,P＜0.01).在PTC中,病理分级Ⅰ级和Ⅱ级组,XAF1蛋白阳性率分别为67.5％(27/40)和40.0％ (12/30),在淋巴结转移阳性和阴性组中分别为35.7％ (10/28)和69.0％(29/42),以上两组比较差异有统计学意义(x2值分别为5.254和7.565,P＜0.05)；XAF1基因启动子甲基化与其蛋白表达有明显的相关性(x2 =8.864,P＜0.01).结论 XAF1基因启动子甲基化是基因失活的重要机制之一,在PTC的发生、发展中起着重要的作用.
目的 探討甲狀腺乳頭狀癌(PTC)組織中X染色體連鎖凋亡抑製因子相關因子1(XAF1)基因啟動子甲基化及其與蛋白錶達的相關性.方法 採用甲基化特異性聚閤酶鏈反應(PCR,MSP)及免疫組化(SP法)檢測70例PTC及其對應的癌徬非癌組織(NCE)中XAF1基因啟動子甲基化及其蛋白的錶達.結果 在NCE中,無一例齣現XAF1基因啟動子甲基化,而在PTC組織中有35.7％ (25/70)齣現甲基化(x2=27.206,P＜0.01),且甲基化狀態與PTC的TNM分期、病理分級及淋巴結轉移有關(P＜0.05)；NCE和PTC中,XAF1蛋白錶達的暘性率分彆為100％ (70/70)和55.7％ (39/70)(x2=36.458,P＜0.01).在PTC中,病理分級Ⅰ級和Ⅱ級組,XAF1蛋白暘性率分彆為67.5％(27/40)和40.0％ (12/30),在淋巴結轉移暘性和陰性組中分彆為35.7％ (10/28)和69.0％(29/42),以上兩組比較差異有統計學意義(x2值分彆為5.254和7.565,P＜0.05)；XAF1基因啟動子甲基化與其蛋白錶達有明顯的相關性(x2 =8.864,P＜0.01).結論 XAF1基因啟動子甲基化是基因失活的重要機製之一,在PTC的髮生、髮展中起著重要的作用.
목적 탐토갑상선유두상암(PTC)조직중X염색체련쇄조망억제인자상관인자1(XAF1)기인계동자갑기화급기여단백표체적상관성.방법 채용갑기화특이성취합매련반응(PCR,MSP)급면역조화(SP법)검측70례PTC급기대응적암방비암조직(NCE)중XAF1기인계동자갑기화급기단백적표체.결과 재NCE중,무일례출현XAF1기인계동자갑기화,이재PTC조직중유35.7％ (25/70)출현갑기화(x2=27.206,P＜0.01),차갑기화상태여PTC적TNM분기、병리분급급림파결전이유관(P＜0.05)；NCE화PTC중,XAF1단백표체적양성솔분별위100％ (70/70)화55.7％ (39/70)(x2=36.458,P＜0.01).재PTC중,병리분급Ⅰ급화Ⅱ급조,XAF1단백양성솔분별위67.5％(27/40)화40.0％ (12/30),재림파결전이양성화음성조중분별위35.7％ (10/28)화69.0％(29/42),이상량조비교차이유통계학의의(x2치분별위5.254화7.565,P＜0.05)；XAF1기인계동자갑기화여기단백표체유명현적상관성(x2 =8.864,P＜0.01).결론 XAF1기인계동자갑기화시기인실활적중요궤제지일,재PTC적발생、발전중기착중요적작용.
Objective To explore the relationship between methylation status of XAF1 (X-linked inhibitor of apoptosis protein associated factor-1 ) gene promoter and its protein expression in papillary thyroid carcinoma (PTC).Methods Methylation-specific polymerase chain reaction (MSP) and immunohistochemical substance P (SP) technique were used to detect the methylation status of XAF1 gene promoter and its protein expression in 70 PTC cases and their matched adjacent non-cancerous epithelium (NCE).Results In NCE,there was no promoter methylation of XAF1 gene while the rate was 35.7％(25/70) in PTC (x2 =27.206,P ＜ 0.01 ).And it was correlated with tumor TNM stage,pathological grade and lymph node metastasis ( P ＜ 0.05 ).The positive rates of XAF1 protein expression in NCE and PTC were 100％ (70/70) and 55.7％ (39/70) respectively.And there was significant difference(x2 =36.458,P ＜ 0.01 ).In PTC,the positive rates of XAF1 protein expression in Grades Ⅰ and Ⅱ were 67.5％(27/40) and40.0％ (12/30) respectively.And they were 35.7％ (10/28) and 69.0％ (29/42) in the lymph node metastasis and non-metastasis groups respectively.And there were significant differences between two groups (P ＜ 0.05 ).Futhermore,there was distinct correlation between methylation of XAF1 gene promoter and its protein expression ( x2 =8.864,P ＜ 0.01 ).Conclusion Methylation of promoter may be one of the important inactivating factors of XAF1 gene.And it plays an important role in the carcinogenesis and progression of PTC.
