中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
20期
1382-1386
,共5页
高勇%刘扬清%曹维克%陈小飞%万一元%衡春%徐丽娟
高勇%劉颺清%曹維剋%陳小飛%萬一元%衡春%徐麗娟
고용%류양청%조유극%진소비%만일원%형춘%서려연
结肠肿瘤%大蒜%细胞增殖%细胞黏附%肿瘤转移
結腸腫瘤%大蒜%細胞增殖%細胞黏附%腫瘤轉移
결장종류%대산%세포증식%세포점부%종류전이
Colonic neoplasms%Garlic%Cell proliferation%Cell adhesion%Neoplasm metastasis志谢江苏省淮安市科技支撑计划(HAS08009)支持
目的 探讨大蒜素对结肠癌LoVo细胞增殖、侵袭和转移能力的影响,及其抗结肠癌侵袭转移作用的机制.方法 采用MTT法检测大蒜素对LoVo细胞增殖抑制能力;肿瘤细胞体外运动实验、黏附实验、迁移实验和Transwell体外侵袭实验检测大蒜素对LoVo细胞运动、黏附、侵袭转移的抑制作用;实时荧光定量RT-PCR法检测大蒜素对LoVo细胞侵袭转移相关基因基质金属蛋白酶2(MMP-2)、基质金属蛋白酶抑制剂2(TIMP-2)、 CD147、血管内皮生长因子(VEGF)、转移抑制基因(nm23-H1)、乙酰肝素酶(HPA)、尿激酶纤维蛋白溶酶原激活剂受体(uPAR)mRNA表达的影响.结果 大蒜素明显抑制人结肠癌LoVo细胞增殖,具有时间剂量依赖性;"无毒"浓度(3和6 μg/ml)大蒜素能够抑制LoVo细胞的体外运动、黏附、侵袭转移能力,作用24 h后对LoVo细胞趋化运动抑制率分别为24%、50%(t=4.543、12.348, P=0.010、0.001),黏附抑制率分别为19%、28% ( t=6.145、 6.355, P=0.004、0.003),迁徙抑制率分别为28%、46%(t=8.065、28.435,均P<0.01),侵袭抑制率分别为44%、65% ( t=21.274、26.288, 均P<0.01);3和6 μg/ml浓度大蒜素能够下调VEGF、uPAR及HPA mRNA的表达(t=7.129、6.764、8.497, P=0.002、0.002、0.001),对TIMP-2, CD147, nm23-H1 mRNA表达无明显影响(t=0.341、1.889、0.914, P=0.059、 0.132、 0.412);LoVo细胞不表达MMP-2 mRNA.结论 "无毒"浓度(3和6 μg/ml)大蒜素对LoVo细胞的迁移运动、黏附、侵袭转移能力有明显抑制作用,该作用可能与抑制VEGF、uPAR及HPA mRNA的表达有关.
目的 探討大蒜素對結腸癌LoVo細胞增殖、侵襲和轉移能力的影響,及其抗結腸癌侵襲轉移作用的機製.方法 採用MTT法檢測大蒜素對LoVo細胞增殖抑製能力;腫瘤細胞體外運動實驗、黏附實驗、遷移實驗和Transwell體外侵襲實驗檢測大蒜素對LoVo細胞運動、黏附、侵襲轉移的抑製作用;實時熒光定量RT-PCR法檢測大蒜素對LoVo細胞侵襲轉移相關基因基質金屬蛋白酶2(MMP-2)、基質金屬蛋白酶抑製劑2(TIMP-2)、 CD147、血管內皮生長因子(VEGF)、轉移抑製基因(nm23-H1)、乙酰肝素酶(HPA)、尿激酶纖維蛋白溶酶原激活劑受體(uPAR)mRNA錶達的影響.結果 大蒜素明顯抑製人結腸癌LoVo細胞增殖,具有時間劑量依賴性;"無毒"濃度(3和6 μg/ml)大蒜素能夠抑製LoVo細胞的體外運動、黏附、侵襲轉移能力,作用24 h後對LoVo細胞趨化運動抑製率分彆為24%、50%(t=4.543、12.348, P=0.010、0.001),黏附抑製率分彆為19%、28% ( t=6.145、 6.355, P=0.004、0.003),遷徙抑製率分彆為28%、46%(t=8.065、28.435,均P<0.01),侵襲抑製率分彆為44%、65% ( t=21.274、26.288, 均P<0.01);3和6 μg/ml濃度大蒜素能夠下調VEGF、uPAR及HPA mRNA的錶達(t=7.129、6.764、8.497, P=0.002、0.002、0.001),對TIMP-2, CD147, nm23-H1 mRNA錶達無明顯影響(t=0.341、1.889、0.914, P=0.059、 0.132、 0.412);LoVo細胞不錶達MMP-2 mRNA.結論 "無毒"濃度(3和6 μg/ml)大蒜素對LoVo細胞的遷移運動、黏附、侵襲轉移能力有明顯抑製作用,該作用可能與抑製VEGF、uPAR及HPA mRNA的錶達有關.
목적 탐토대산소대결장암LoVo세포증식、침습화전이능력적영향,급기항결장암침습전이작용적궤제.방법 채용MTT법검측대산소대LoVo세포증식억제능력;종류세포체외운동실험、점부실험、천이실험화Transwell체외침습실험검측대산소대LoVo세포운동、점부、침습전이적억제작용;실시형광정량RT-PCR법검측대산소대LoVo세포침습전이상관기인기질금속단백매2(MMP-2)、기질금속단백매억제제2(TIMP-2)、 CD147、혈관내피생장인자(VEGF)、전이억제기인(nm23-H1)、을선간소매(HPA)、뇨격매섬유단백용매원격활제수체(uPAR)mRNA표체적영향.결과 대산소명현억제인결장암LoVo세포증식,구유시간제량의뢰성;"무독"농도(3화6 μg/ml)대산소능구억제LoVo세포적체외운동、점부、침습전이능력,작용24 h후대LoVo세포추화운동억제솔분별위24%、50%(t=4.543、12.348, P=0.010、0.001),점부억제솔분별위19%、28% ( t=6.145、 6.355, P=0.004、0.003),천사억제솔분별위28%、46%(t=8.065、28.435,균P<0.01),침습억제솔분별위44%、65% ( t=21.274、26.288, 균P<0.01);3화6 μg/ml농도대산소능구하조VEGF、uPAR급HPA mRNA적표체(t=7.129、6.764、8.497, P=0.002、0.002、0.001),대TIMP-2, CD147, nm23-H1 mRNA표체무명현영향(t=0.341、1.889、0.914, P=0.059、 0.132、 0.412);LoVo세포불표체MMP-2 mRNA.결론 "무독"농도(3화6 μg/ml)대산소대LoVo세포적천이운동、점부、침습전이능력유명현억제작용,해작용가능여억제VEGF、uPAR급HPA mRNA적표체유관.
Objective To study the effect of allicin on invasion and metastasis of human colon cancer cell line LoVo in vitro and furthermore elucidate its anticancer mechanisms. Methods MTT assay was used to test dynamically the effect of cell growth inhibition.The inhibitory effects of allicin on movement, adhesiveness and invasiveness of LoVo cells were evaluated by the migratory test, adhesion test and Transwell chamber experiment.Quantitative real-time reverse transcription PCR (real-time RT-PCR) was performed to quantify the mRNA expression of MMP-2, TIMP-2, CD147, VEGF, nm23-H1, HPA and uPAR. Results Allicin had inhibitive effects on growth of LoVo cells in a dose and time-dependent manner.Allicin at non-cytotoxic concentration (3 and 6μg/ml) could obviously suppress the movement, adhesion and invasive capability of LoVo cells.In the allicin-treated group(3 and 6 μg/ml), after 24 hours, the inhibition rates of migratory time were 24% and 50%(t=4.543, 12.348, P=0.010, 0.001), the inhibition rates of adhesion were 19% and 28%(t=6.145, 6.355, P=0.004, 0.003), the inhibition rates of migration were 28% and 46%(t=8.065, 28.435,both P<0.01), and the inhibition rates of invasion were 44% and 65% respectively (t=21.274, 26.288, both P<0.01).Allicin at non-cytotoxic concentration could down-regulate the mRNA levels of VEGF, uPAR and HPA in a dose-dependent manner in LoVo cells (t=7.129, 6.764, 8.497, P=0.002, 0.002, 0.001) while the mRNA levels of TIMP-2, CD147 and nm23-H1 remained basically unchanged with the same treatment (t=0.341, 1.889, 0.914, P=0.059, 0.132, 0.412).The expression of MMP-2 had not been detected in LoVo cells. Conclusion Allicin in vitro inhibits invasion and metastasis of human colon carcinoma cell LoVo at non-cytotoxic concentration through down-regulating the expression of VEGF, uPAR and HPA mRNA.
