解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2002年
1期
11-15
,共5页
三叉神经本体觉%5-羟色胺%逆行束路追踪%免疫细胞化学%大鼠
三扠神經本體覺%5-羥色胺%逆行束路追蹤%免疫細胞化學%大鼠
삼차신경본체각%5-간색알%역행속로추종%면역세포화학%대서
Trigeminal proprioception%Serotonin%Retrograde-tra ct tracing%Immunocytochemistry%Rat
目的研究5-羟色胺(5-HT)样免疫反应纤维终末与大鼠三叉神经本体觉中枢通路二级神经元之间是否存在突触联系. 方法逆行束路追踪与免疫组织化学相结合的电镜双重标记技术. 结果将麦芽凝集素结合的辣根过氧化物酶(WGA-HRP)注入大鼠三叉神经感觉主核背内侧部(Vpdm)并进行5-HT免疫染色后,在三叉神经脊束核吻侧亚核背内侧部及其邻接的外侧网状结构(Vodm-LRF)中可见WGA-HRP逆行标记的神经元和5-HT样阳性轴突终末.电镜下观察到5-HT样阳性轴突终末与WGA-HRP标记的神经元之间有轴-体、轴-树突触联系,这些突触属对称或非对称型,但以对称型为主. 结论本研究为5-HT能终末可能对三叉神经本体觉信息的传递具有一定的调控作用提供了形态学依据.
目的研究5-羥色胺(5-HT)樣免疫反應纖維終末與大鼠三扠神經本體覺中樞通路二級神經元之間是否存在突觸聯繫. 方法逆行束路追蹤與免疫組織化學相結閤的電鏡雙重標記技術. 結果將麥芽凝集素結閤的辣根過氧化物酶(WGA-HRP)註入大鼠三扠神經感覺主覈揹內側部(Vpdm)併進行5-HT免疫染色後,在三扠神經脊束覈吻側亞覈揹內側部及其鄰接的外側網狀結構(Vodm-LRF)中可見WGA-HRP逆行標記的神經元和5-HT樣暘性軸突終末.電鏡下觀察到5-HT樣暘性軸突終末與WGA-HRP標記的神經元之間有軸-體、軸-樹突觸聯繫,這些突觸屬對稱或非對稱型,但以對稱型為主. 結論本研究為5-HT能終末可能對三扠神經本體覺信息的傳遞具有一定的調控作用提供瞭形態學依據.
목적연구5-간색알(5-HT)양면역반응섬유종말여대서삼차신경본체각중추통로이급신경원지간시부존재돌촉련계. 방법역행속로추종여면역조직화학상결합적전경쌍중표기기술. 결과장맥아응집소결합적랄근과양화물매(WGA-HRP)주입대서삼차신경감각주핵배내측부(Vpdm)병진행5-HT면역염색후,재삼차신경척속핵문측아핵배내측부급기린접적외측망상결구(Vodm-LRF)중가견WGA-HRP역행표기적신경원화5-HT양양성축돌종말.전경하관찰도5-HT양양성축돌종말여WGA-HRP표기적신경원지간유축-체、축-수돌촉련계,저사돌촉속대칭혹비대칭형,단이대칭형위주. 결론본연구위5-HT능종말가능대삼차신경본체각신식적전체구유일정적조공작용제공료형태학의거.
Objective To investigate whether the serotonin(5-HT)-like immunoreactive axons synapse upon the second-order neurons on the central path way of the trigeminal proprioception in the rat. Methods Electron microscopic double-labeling of retrograde tract-tracing technique combined with immunohistochemistry. Results Following the injection of wheat germ agglutinin-horsera dish peroxidase (WGA-HRP) into the dorsomedial part of the principal sensory tr igeminal nucleus(Vpdm) of the rat and the immunostaining for 5-HT, there were n eurons retrogradely labeled by WGA-HRP and axon terminals with 5-HT-like immu noreactivity(5-HT-LI) in the dorsomedial part of the subnucleus oralis of the spinal trigeminal nucleus and its adjacent lateral reticular formation(Vodm-LRF ).At the electron microscopic level,the 5-HT-immunostained axonal profiles wer e observed to make synaptic contacts with the WGA-HRP-labeled neurons.Both the axon-somatic and axon-dendritic synapses were found.These synapses were mainly of symmetric type although the asymmetric ones were also seen.Conclusion The present results provided a morphologic basis for the serotoninergic terminals which might be involved in the regulatory effect for t he trigeminal proprioceptive information transmission.