CAJ | 학술논문

目的考察银杏内酯B(ginkgolide B,BN52021)对血小板活化因子(PAF)引起的小鼠腹腔巨噬细胞趋化反应和丝状肌动蛋白(F-actin)聚合作用的影响.方法Boyden小室法检测化合物对巨噬细胞趋化的影响;流式细胞术检测特异性标记的巨噬细胞中F-actin的变化.结果PAF可显著刺激小鼠腹腔巨噬细胞产生趋化效应,PAF受体拮抗剂BN52021(0.01 nml·L-1～0.1μmol·L-1)可明显抑制该作用.此外,在含钙缓冲液中,BN52021可显著抑制PAF引起的丝状肌动蛋白聚合.结论BN52021可能通过抑制丝状肌动蛋白的聚合作用,从而抑制PAF引起的巨噬细胞趋化,并且这种作用是钙依赖性的.表明BN52021的抗炎作用途径之一是抑制PAF诱导的趋化作用.
목적고찰은행내지B(ginkgolide B,BN52021)대혈소판활화인자(PAF)인기적소서복강거서세포추화반응화사상기동단백(F-actin)취합작용적영향.방법Boyden소실법검측화합물대거서세포추화적영향;류식세포술검측특이성표기적거서세포중F-actin적변화.결과PAF가현저자격소서복강거서세포산생추화효응,PAF수체길항제BN52021(0.01 nml·L-1～0.1μmol·L-1)가명현억제해작용.차외,재함개완충액중,BN52021가현저억제PAF인기적사상기동단백취합.결론BN52021가능통과억제사상기동단백적취합작용,종이억제PAF인기적거서세포추화,병차저충작용시개의뢰성적.표명BN52021적항염작용도경지일시억제PAF유도적추화작용.
Aim To study the inhibitory effect of ginkgolide B (BN52021) on the PAF induced changes of chemotaxis of murine peritoneal macrophages and the related polymerization of F-actin.Methods Chemotaxis assays were performed using a modified 48-well Boyden chamber. Actin polymerization of murine peritoneal macrophages was analyzed by flow cytometry using a specific fluorescent stain. Results Peritoneal macrophages significantly migrated toward platelet-activating factor(PAF) through a micropore filter; however, in the presence of PAF receptor antagonist BN52021 (0. 01the actin polymerization of murine peritoneal macrophages induced by PAF in the presence of Ca2+ , but not in Ca2+ -free medium. Conclusion The results suggested that preventing polymerization of F-actin may be a pathway by BN52021 to inhibit the chemotaxis of macrophages, and this effect seems to be Ca2+dependent. The data further indicated that inhibition of PAF induced macrophage chemotaxis is an important mechanism underlying the anti-inflammatory action of BN52021.