中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2007年
30期
6103-6105
,共3页
胡电%古航%洪新如%金志军%梁永%熊英
鬍電%古航%洪新如%金誌軍%樑永%熊英
호전%고항%홍신여%금지군%량영%웅영
抑郁症,产后%脑啡肽类%生物胺神经递质类
抑鬱癥,產後%腦啡肽類%生物胺神經遞質類
억욱증,산후%뇌배태류%생물알신경체질류
背景:孤啡肽是近年来才发现的一种重要的生物活性多肽,其在中枢及外周的广泛分布提示它可能有较为广泛的调节整体行为的功能.目的:测定产后抑郁症妇女血孤啡肽及单胺类递质5-羟色胺、多巴胺的含量,探讨产后抑郁症时孤啡肽含量的变化及意义.设计:以诊断为依据的病例对照实验.单位:解放军第二军医大学长征及长海医院妇产科.对象:2002-02/2004-10在解放军第二军医大学长征,长海医院妇产科门诊产后复诊的健康产妇25例(对照组)及发生产后抑郁症的产妇17例(抑郁组).均为自愿参加者,年龄为21～43岁,文化程度相当.抑郁组均为首次发病,发病时间为产后15～25 d,爱丁堡产后抑郁量表总分≥13分.试验对象对本试验的内容知情同意.方法:测定25例健康产妇(对照组)及17例产后抑郁症妇女(抑郁组)静脉血中孤啡肽及单胺类递质含量.①标本采集:抑郁组于发病2周后取血,对照组于产后35 d抽血.②测定方法:采用放射免疫测定法测定孤啡肽含量,药盒由美国phoenix公司提供,操作程序严格按药盒说明进行;5-羟色胺及多巴胺测定采用高压液相色谱/电化学检测法.主要观察指标:①抑郁组及对照组血孤啡肽含量及5-羟色胺、多巴胺含量的变化.②抑郁组爱丁保产后抑郁量表总分与孤啡肽含量的相关性.结果:对照组25例健康产妇及抑郁组17例发生产后抑郁症的产妇均纳入结果分析.①与对照组相比,抑郁组孤啡肽含量升高,而5-羟色胺及多巴胺含量则下降(抑郁组及对照组血孤啡肽:(27.39±6.04),(10.37±3.65)ng/L;抑郁组及对照组血 5-羟色胺:(0.93±0.21),(1.43±0.36)μmol/L;抑郁组及对照组血多巴胺:(2.15±0.41),(3.64±0.72)μmol/L,P＜0.01或0.05).②抑郁组孤啡肽与5-羟色胺及多巴胺含量呈负相关(r为-0.601及-0.593,P＜0.05).③抑郁组爱丁堡产后抑郁量表总分与孤啡肽含量呈正相关(r=0.512,P＜0.05),与5-羟色胺、多巴胺含量呈负相关(r=-0.571,-0.526,P＜0.05).结论:产后抑郁患者血孤啡肽含量增加并与5-羟色胺及多巴胺的含量呈负向相关,与爱丁堡产后抑郁量表评分呈正向相关.
揹景:孤啡肽是近年來纔髮現的一種重要的生物活性多肽,其在中樞及外週的廣汎分佈提示它可能有較為廣汎的調節整體行為的功能.目的:測定產後抑鬱癥婦女血孤啡肽及單胺類遞質5-羥色胺、多巴胺的含量,探討產後抑鬱癥時孤啡肽含量的變化及意義.設計:以診斷為依據的病例對照實驗.單位:解放軍第二軍醫大學長徵及長海醫院婦產科.對象:2002-02/2004-10在解放軍第二軍醫大學長徵,長海醫院婦產科門診產後複診的健康產婦25例(對照組)及髮生產後抑鬱癥的產婦17例(抑鬱組).均為自願參加者,年齡為21～43歲,文化程度相噹.抑鬱組均為首次髮病,髮病時間為產後15～25 d,愛丁堡產後抑鬱量錶總分≥13分.試驗對象對本試驗的內容知情同意.方法:測定25例健康產婦(對照組)及17例產後抑鬱癥婦女(抑鬱組)靜脈血中孤啡肽及單胺類遞質含量.①標本採集:抑鬱組于髮病2週後取血,對照組于產後35 d抽血.②測定方法:採用放射免疫測定法測定孤啡肽含量,藥盒由美國phoenix公司提供,操作程序嚴格按藥盒說明進行;5-羥色胺及多巴胺測定採用高壓液相色譜/電化學檢測法.主要觀察指標:①抑鬱組及對照組血孤啡肽含量及5-羥色胺、多巴胺含量的變化.②抑鬱組愛丁保產後抑鬱量錶總分與孤啡肽含量的相關性.結果:對照組25例健康產婦及抑鬱組17例髮生產後抑鬱癥的產婦均納入結果分析.①與對照組相比,抑鬱組孤啡肽含量升高,而5-羥色胺及多巴胺含量則下降(抑鬱組及對照組血孤啡肽:(27.39±6.04),(10.37±3.65)ng/L;抑鬱組及對照組血 5-羥色胺:(0.93±0.21),(1.43±0.36)μmol/L;抑鬱組及對照組血多巴胺:(2.15±0.41),(3.64±0.72)μmol/L,P＜0.01或0.05).②抑鬱組孤啡肽與5-羥色胺及多巴胺含量呈負相關(r為-0.601及-0.593,P＜0.05).③抑鬱組愛丁堡產後抑鬱量錶總分與孤啡肽含量呈正相關(r=0.512,P＜0.05),與5-羥色胺、多巴胺含量呈負相關(r=-0.571,-0.526,P＜0.05).結論:產後抑鬱患者血孤啡肽含量增加併與5-羥色胺及多巴胺的含量呈負嚮相關,與愛丁堡產後抑鬱量錶評分呈正嚮相關.
배경:고배태시근년래재발현적일충중요적생물활성다태,기재중추급외주적엄범분포제시타가능유교위엄범적조절정체행위적공능.목적:측정산후억욱증부녀혈고배태급단알류체질5-간색알、다파알적함량,탐토산후억욱증시고배태함량적변화급의의.설계:이진단위의거적병례대조실험.단위:해방군제이군의대학장정급장해의원부산과.대상:2002-02/2004-10재해방군제이군의대학장정,장해의원부산과문진산후복진적건강산부25례(대조조)급발생산후억욱증적산부17례(억욱조).균위자원삼가자,년령위21～43세,문화정도상당.억욱조균위수차발병,발병시간위산후15～25 d,애정보산후억욱량표총분≥13분.시험대상대본시험적내용지정동의.방법:측정25례건강산부(대조조)급17례산후억욱증부녀(억욱조)정맥혈중고배태급단알류체질함량.①표본채집:억욱조우발병2주후취혈,대조조우산후35 d추혈.②측정방법:채용방사면역측정법측정고배태함량,약합유미국phoenix공사제공,조작정서엄격안약합설명진행;5-간색알급다파알측정채용고압액상색보/전화학검측법.주요관찰지표:①억욱조급대조조혈고배태함량급5-간색알、다파알함량적변화.②억욱조애정보산후억욱량표총분여고배태함량적상관성.결과:대조조25례건강산부급억욱조17례발생산후억욱증적산부균납입결과분석.①여대조조상비,억욱조고배태함량승고,이5-간색알급다파알함량칙하강(억욱조급대조조혈고배태:(27.39±6.04),(10.37±3.65)ng/L;억욱조급대조조혈 5-간색알:(0.93±0.21),(1.43±0.36)μmol/L;억욱조급대조조혈다파알:(2.15±0.41),(3.64±0.72)μmol/L,P＜0.01혹0.05).②억욱조고배태여5-간색알급다파알함량정부상관(r위-0.601급-0.593,P＜0.05).③억욱조애정보산후억욱량표총분여고배태함량정정상관(r=0.512,P＜0.05),여5-간색알、다파알함량정부상관(r=-0.571,-0.526,P＜0.05).결론:산후억욱환자혈고배태함량증가병여5-간색알급다파알적함량정부향상관,여애정보산후억욱량표평분정정향상관.
BACKGROUND: Orphanin, which was found in recent years, is an important bioactive polypeptide. Its extensive distribution in the central nervous system and peripheral tissue suggests a function of adjusting global behaviors.OBJECTIVE: To explore the correlation of plasm orphanin level and monoamine neurotransmitters in patients with postpartum depression by determining the levels of orphanin and monoamine neurotransmitters such as 5-hydroxytryptamine (5-HT) and dopamine(DA) in patients.DESIGN: Case-control experiment taking diagnosis as evidence.SETTING: Departments of Obstetrics and Gynecology, Changzheng Hospital and Changhai Hospital, Second Military Medical University of Chinese PLA.PARTICIPANTS: Twenty-five healthy women and seventeen women patients with postpartum depression after delivery who came in the Department of Obstetrics and Gynecology, Changzheng Hospital and Changhai Hospital, Second Military Medical University of Chinese PLA for further consultation during February 2002 to October 2004 were enrolled in this study, and served as control group and postpartum depression group, respectively. All the enrolled women, aged 21 to 43 years, with fair education levels, and voluntarily participated in this study. Women patients of 15 to 25 days after delivery suffered from the incipient postpartum depression with total scores of Edingburgh Postnatal Depression Scale (EPDS) above 13. Informed consents were obtained from all the subjects.METHODS: The levels of orphanin and monoamine neurotransmitters in venous blood from 25 healthy women and 17 women patients with postpartum depression were determined. ① Sample collecting: Blood was taken from women in the postpartum depression group 2 weeks after onset, while from control group 35 days after delivery. ② Assay method:The level of orphanin was determined with radioimmunoassay (RIA). The reagent kit was provided by phoenix company (USA) and the procedure was strictly performed according to the instructions on the reagent kit. The levels of 5-HT and DA were measured with high-performance liquid chromatography or electrochemical detection method.MAIN OUTCOME MEASURES: ① Changes in the levels of orphanin as well as 5-HT and DA in postpartum depression group and control group. ② Correlation of the total scores of EPDS and the levels of orphanin in postpartum depression group.RESULTS: Twenty-five healthy women and seventeen women patients with postpartum depression after delivery all participated in the result analysis. ① The level of orphanin in the postpartum depression group[(27.39±6.04) ng/L] was significantly higher than that in the control group [(10.37±3.65) ng/L](P ＜ 0.01),the levels of 5-HT in the postpartum depression group [(0.93±0.21) μmol/L] was remarkably lower than that in the control group[(1.43±0.36) μmol/L](P ＜0.05) ,and the levels of DA in postpartum depression group[(2.15±0.41) μmol/L] was obviously lower than that in the control group [(3.64±0.72) μmol/L]( P ＜ 0.01). ②The level of orphanin was negatively correlated with those of 5-HT and DA in postpartum depression group(r = -0.601 , -0.593,P ＜ 0.05). ③ The total scores of EPDS were significantly positively correlated with the level of orphanin (r = 0.512, P ＜ 0.05), and negatively correlated with the levels of 5-HT and DA (r = -0.571,-0.526, P＜ 0.05).CONCLUSION: The level of orphanin in patients with postpartum depression is increased and is negatively correlated with the levels of 5-HT and DA, but positively correlated with the total scores of EPDS.
