CAJ | 학술논문

目的研究特发性血小板减少性紫癜(ITP)患者外周血CD4+CD25+调节性T细胞(Tr细胞)的变化,探讨其在ITP发病机制中的作用.方法分别收集35例ITP患者及健康对照组外周抗凝静脉血,分离纯化T淋巴细胞.应用藻红蛋白(PE)标记的抗CD4单抗及异硫氰酸荧光素(FITC)标记的抗CD25单抗,作双色流式细胞术,分析ITP患者外周血CD4+CD25+调节性T细胞百分率.结果 ITP组外周血中Tr细胞明显低于健康对照组(P<0.05),Tr细胞占CD4+T细胞的比例也低于健康对照组(P<0.05).结论 ITP患者外周血存在细胞免疫功能失调,Tr细胞数量减少可能与ITP的发病机制有关.
목적 연구특발성혈소판감소성자전(ITP)환자외주혈CD4+CD25+조절성T세포(Tr세포)적변화,탐토기재ITP발병궤제중적작용.방법 분별수집35례ITP환자급건강대조조외주항응정맥혈,분리순화T림파세포.응용조홍단백(PE)표기적항CD4단항급이류청산형광소(FITC)표기적항CD25단항,작쌍색류식세포술,분석ITP환자외주혈CD4+CD25+조절성T세포백분솔.결과 ITP조외주혈중Tr세포명현저우건강대조조(P<0.05),Tr세포점CD4+T세포적비례야저우건강대조조(P<0.05).결론 ITP환자외주혈존재세포면역공능실조,Tr세포수량감소가능여ITP적발병궤제유관.
Objective To investigate the change of peripheral blood CD4+CD25+ regulatory T cells (Tr) in patients with idiopathic thrombocytopenic purpura (ITP), and to analyze its role in the pathogenesis of ITP.Methods Anticoagulated venous blood was collected from ITP patients (ITP group,n=35) and healthy controls (healthy control group,n=35). T lymphocytes were isolated and purified with human CD3+ T cell enrichment columm. The percentage of peripheral blood CD4+CD25+ regulatory T cells was detected by immunofluorescence staining (PE-anti-CD4 monoclone antibody and FITC-CD25 monoclone antibody) and bicolor flow cytometry by CD25/CD4 gating.Results The number and constituent ratio of CD4+CD25+T cell were significantly lower in ITP group than those of healthy control group (P<0.05).Conclusion There is peripheral blood celluar immunological function disorder in ITP patients, and decrease of CD4+CD25+ T cell population may be involved in the pathogenesis of ITP.