CAJ | 학술논문

从全国13个省市自治区的70份土样中分离、采集到了非共生固氮微生物资源181份.从形态特征、生理生化特征和16S rDNA序列分析表明.采集到的菌种资源在科学分类上属于24属66种,大约占到已报道非共生固氮微生物属的一半,具备一定的多样性和代表性.资源在分类学上的特点是分类地位相对集中,有65株菌属于类芽孢杆菌属,占总量的36%;52株菌属于芽孢杆菌属,占总量的29%;19株菌属于节杆菌属,占总量的11%;这3个属菌株合计占采集资源总量的76%.随地域和作物种类分布的特点是芽孢杆菌和类芽孢杆菌两个属的菌种资源具有很强的地域广泛性和作物广泛性,即从采自全同符地、各种作物的土壤样品几乎都可以分离到这两类菌种.这个研究结果对微生物肥料菌种选育和生产应用具有指导意义.
종전국13개성시자치구적70빈토양중분리、채집도료비공생고담미생물자원181빈.종형태특정、생리생화특정화16S rDNA서렬분석표명.채집도적균충자원재과학분류상속우24속66충,대약점도이보도비공생고담미생물속적일반,구비일정적다양성화대표성.자원재분류학상적특점시분류지위상대집중,유65주균속우류아포간균속,점총량적36%;52주균속우아포간균속,점총량적29%;19주균속우절간균속,점총량적11%;저3개속균주합계점채집자원총량적76%.수지역화작물충류분포적특점시아포간균화류아포간균량개속적균충자원구유흔강적지역엄범성화작물엄범성,즉종채자전동부지、각충작물적토양양품궤호도가이분리도저량류균충.저개연구결과대미생물비료균충선육화생산응용구유지도의의.
One hundred and eighty one asymbiotic nitrogen-fixing bacterial strains were isolated from 70 soil samples collected from 13 provinces over China. Results based on the determination of morphology, physiology and chemical proper-ties, together with 16S rDNA sequencing, indicated that these strains belong to 66 species of 24 genera, which account for about a half of currently reported genera of asymbiotic nitrogen-fixing bacteria. Taxonomic distribution of these strains was relative converging, 65 strains belongs to Paenibacillus, 52 strains belongs to Bacillus and 19 strains belongs to Arthrobacter, which accounts for 36%, 29% and 11% of gross resource, respectively. Strains of these 3 groups make up 76% of gross esource. Strains of Paenibacillus and Bacillus showed significant diversity on the distribution regarding to geography and crops. Namely strains of these 2 groups could be isolated from almost every sample collected from root area of crops all over China.