CAJ | 학술논문

[目的]了解在新疆泥火山群这一特殊生境条件下一株中度嗜盐菌种属关系.[方法]从新疆泥火山分离得到中度嗜盐菌WS1,测定其生理生化指标,并采用真细菌16S rDNA通用引物PCR扩增WS1菌株的16S rDNA,将得到的片段在NCBI Genbank数据库中通过 Blast软件对其进行同源性检索,使用PHYLIP3.65软件构建系统发育树.[结果]该菌能够在浓度0～15%NaCl的条件下生长;PCR扩增得到的片段长度为1 423 bp;同源性比对表明,该菌株与多种涅斯捷连科氏菌的16S rDNA序列同源性高达99%;系统发育树显示WS1菌株与涅斯捷连科氏菌的亲缘关系最近.[结论]结合生理生化指标,初步确定WS1菌株可能是耐盐涅斯捷连科氏菌属的一个亚种.
[목적]료해재신강니화산군저일특수생경조건하일주중도기염균충속관계.[방법]종신강니화산분리득도중도기염균WS1,측정기생리생화지표,병채용진세균16S rDNA통용인물PCR확증WS1균주적16S rDNA,장득도적편단재NCBI Genbank수거고중통과 Blast연건대기진행동원성검색,사용PHYLIP3.65연건구건계통발육수.[결과]해균능구재농도0～15%NaCl적조건하생장;PCR확증득도적편단장도위1 423 bp;동원성비대표명,해균주여다충열사첩련과씨균적16S rDNA서렬동원성고체99%;계통발육수현시WS1균주여열사첩련과씨균적친연관계최근.[결론]결합생리생화지표,초보학정WS1균주가능시내염열사첩련과씨균속적일개아충.
[Objective]The research aimed to study the relationship of a moderately halophilic bacteria in this special habitat condition.[Method] The moderately halophilic bacteria WS1 was isolated from mud volcano in Xinjiang. Physiological and biochemical indexes were measured. The universal primers for the 16S rDNA of eubacteria were used for polymerase chain reaction. Homology analysis was made by searching in the NCBI Genbank database with Blast software. The phylogenetic tree was constructed with PHYLIP3.65. [Result]Strain WS1 could grow on the media containing 0%-15% NaCl. The received fragment length was 1 423 bp. The homology analysis showed that 16S rDNA homologies was 99% between strain WS1 and many kinds of Nesterenkonia bacteriums. The phylogenetic tree showed that the relationship between strain WS1 and Nesterenkonia bacterium was the closest.[Conclusion]Combination analysis of physiological and biochemical, the strain WS1 was identified as a subspecies of salt-tolerant Nesterenkonia.