国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
7期
617-620
,共4页
王丽华%于俊生%孙云松%刘先英%陈芊
王麗華%于俊生%孫雲鬆%劉先英%陳芊
왕려화%우준생%손운송%류선영%진천
糖尿病肾病%祛痰通络汤%转化生长因子-β1
糖尿病腎病%祛痰通絡湯%轉化生長因子-β1
당뇨병신병%거담통락탕%전화생장인자-β1
Diabetic nephropathies%Qutantongluo decoction%TGF-β1
目的 观察祛痰通络汤对糖尿病肾病(diabetic nephropathies,DN)模型大鼠肾组织转化 生长因子-β1 (TGF-β1)表达的影响.方法 制作糖尿病肾病大鼠模型.按随机数字表法将大鼠随机分为6组:正常对照组、模型对照组、贝那普利组(1 mg/kg·d-1)、祛痰通络汤低剂量组(3g/kg·d-1)、祛痰通络汤中剂量组(4.5 g/kg·d-1)、祛痰通络汤高剂量组(6g/kg·d-1),正常对照组和模型对照组每日均在同一时间予同体积蒸馏水灌胃.各组分别干预8周,采用免疫组化、RT-PCR方法分别检测各组肾组织TGF-β1蛋白和基因水平.结果 肾组织TGF-β1蛋白表达分别为:贝那普利组(1.55±0.12)、祛痰通络汤低剂量组( 1.54±0.16)、祛痰通络汤中剂量组(1.49±0.17)、祛痰通络汤高剂量组(1.39±0.25),均低于模型对照组(2.79±0.22),(P均＜0.05)；肾组织TGF-β1 mRNA的表达分别为贝那普利组(0.35±0.07)、祛痰通络汤低剂量组(0.39±0.03)、祛痰通络汤中剂量组(0.35±0.06)、祛痰通络汤高剂量组(0.32±0.07),均低于模型对照组(0.65±0.09),(P均＜0.05).结论 祛痰通络汤下调TGF-β1在肾组织的表达,可能是祛痰通络汤治疗DN的作用机制之一.
目的 觀察祛痰通絡湯對糖尿病腎病(diabetic nephropathies,DN)模型大鼠腎組織轉化 生長因子-β1 (TGF-β1)錶達的影響.方法 製作糖尿病腎病大鼠模型.按隨機數字錶法將大鼠隨機分為6組:正常對照組、模型對照組、貝那普利組(1 mg/kg·d-1)、祛痰通絡湯低劑量組(3g/kg·d-1)、祛痰通絡湯中劑量組(4.5 g/kg·d-1)、祛痰通絡湯高劑量組(6g/kg·d-1),正常對照組和模型對照組每日均在同一時間予同體積蒸餾水灌胃.各組分彆榦預8週,採用免疫組化、RT-PCR方法分彆檢測各組腎組織TGF-β1蛋白和基因水平.結果 腎組織TGF-β1蛋白錶達分彆為:貝那普利組(1.55±0.12)、祛痰通絡湯低劑量組( 1.54±0.16)、祛痰通絡湯中劑量組(1.49±0.17)、祛痰通絡湯高劑量組(1.39±0.25),均低于模型對照組(2.79±0.22),(P均＜0.05)；腎組織TGF-β1 mRNA的錶達分彆為貝那普利組(0.35±0.07)、祛痰通絡湯低劑量組(0.39±0.03)、祛痰通絡湯中劑量組(0.35±0.06)、祛痰通絡湯高劑量組(0.32±0.07),均低于模型對照組(0.65±0.09),(P均＜0.05).結論 祛痰通絡湯下調TGF-β1在腎組織的錶達,可能是祛痰通絡湯治療DN的作用機製之一.
목적 관찰거담통락탕대당뇨병신병(diabetic nephropathies,DN)모형대서신조직전화 생장인자-β1 (TGF-β1)표체적영향.방법 제작당뇨병신병대서모형.안수궤수자표법장대서수궤분위6조:정상대조조、모형대조조、패나보리조(1 mg/kg·d-1)、거담통락탕저제량조(3g/kg·d-1)、거담통락탕중제량조(4.5 g/kg·d-1)、거담통락탕고제량조(6g/kg·d-1),정상대조조화모형대조조매일균재동일시간여동체적증류수관위.각조분별간예8주,채용면역조화、RT-PCR방법분별검측각조신조직TGF-β1단백화기인수평.결과 신조직TGF-β1단백표체분별위:패나보리조(1.55±0.12)、거담통락탕저제량조( 1.54±0.16)、거담통락탕중제량조(1.49±0.17)、거담통락탕고제량조(1.39±0.25),균저우모형대조조(2.79±0.22),(P균＜0.05)；신조직TGF-β1 mRNA적표체분별위패나보리조(0.35±0.07)、거담통락탕저제량조(0.39±0.03)、거담통락탕중제량조(0.35±0.06)、거담통락탕고제량조(0.32±0.07),균저우모형대조조(0.65±0.09),(P균＜0.05).결론 거담통락탕하조TGF-β1재신조직적표체,가능시거담통락탕치료DN적작용궤제지일.
Objective To observe the effects of Qutantongluo decoction on the expression of TGF-β1 in renal tissue of diabetic nephropathy(DN) rats,which can help to understand the mechanism of action.Methods The rats models of diabetic nephropathy were established by injecting with streptozotocin intraperi-toneally.All Wistar rats were randomly divided into 6 groups:the normal control group,the model control group,Benazepril Hydrochloridec group ( 1 mg/kg ·d-1 ),Qutantongluo decoction low dose group (3 g/kg ·d-1 ),Qutantongluo decoction median dose group (4.5 g/kg · d-1 ),and Qutantongluo decoction high dose group (6 g/kg · d-1 ).Rats in the normal control group and the model control group were given celiac perfusion of distilled water once at the same time and dose.All the rats were gavaged once daily for 8 weeks.The expression of TGF-β1 at the level of gene and protein in renal tissue were tested by immunohistochemistry and RT-PCR methods.Results Compared with the model control group(2.79±0.22),the expression of TGF-β1 at the level of protein in renal tissue of Benazepril Hydrochloridec group (1.55 ±0.12)and the Qutantongluo decoction low dose group ( 1.54± 0.16),Qutantongluo decoction median dose group (1.49 ± 0.17),Qutantongluo decoction high dose group(l.39±0.25) decreased significantly (P＜0.05).Compared with the model control group (0.35±0.07),the expression of TGF-β1 mRNA in renal tissue of Benazepril Hydrochloridec group(0.35± 0.07)and the Qutantongluo decoction low dose group(0.39±0.03),Qutantongluo decoction median dose group (0.35± 0.06),Qutantongluo decoction high dose group (0.32± 0.07) decreased significantly (P＜0.05).Conclusion Qutantongluo decoction can down regulate the expression of TGF-β1 in kidney,which may be one of the mechanisms of Qutantongluo decoction effectiveness in clinical treatment of diabetic nephropathies.
