中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2010年
11期
848-852
,共5页
周涌%李富宇%苏兰%蒋力生%程南生%李宁%李全生%何生
週湧%李富宇%囌蘭%蔣力生%程南生%李寧%李全生%何生
주용%리부우%소란%장력생%정남생%리저%리전생%하생
胆结石%慢性增生性胆管炎%复发%胆道狭窄%PCNA
膽結石%慢性增生性膽管炎%複髮%膽道狹窄%PCNA
담결석%만성증생성담관염%복발%담도협착%PCNA
Cholelithiasis%Proliferative cholangitis%Recurrence%Biliary duct restenosis%PCNA
目的 近年的研究证实胆结石的术后高复发率和胆道再狭窄率均与术后遗留的慢性增生性胆管炎(PC)密切相关,鉴于PC是一种过度增殖疾病,本研究拟探讨应用PCNA shRNA抑制PC的过度增殖行为和成石潜力的可行性和疗效,以期为预防结石的术后复发和胆道再狭窄探讨新的治疗途径.方法 建立慢性增生性胆管炎的实验动物模型,胆总管内注入0.5 ml的PCNA shRNA,探讨其对PC的过度增殖行为和成石潜力的影响.结果 PCNA shRNA可通过抑制PCNA和Procollagen Ⅲ mRNA和蛋白的表达,有效抑制胆道黏膜上皮、黏膜下腺体和胶原纤维的过度增殖,有望达到控制或逆转慢性增生性胆管炎及其所继发的胆道狭窄的目的 .此外,该治疗还可抑制黏蛋白基因Mucin 5AC的激活和表达,有助于降低PC的成石潜力.结论 PCNA shRNA治疗可能在达到控制或逆转慢性增生性胆管炎及其所继发的胆道狭窄的目的 同时,亦有助于降低病变胆管的成石潜力.
目的 近年的研究證實膽結石的術後高複髮率和膽道再狹窄率均與術後遺留的慢性增生性膽管炎(PC)密切相關,鑒于PC是一種過度增殖疾病,本研究擬探討應用PCNA shRNA抑製PC的過度增殖行為和成石潛力的可行性和療效,以期為預防結石的術後複髮和膽道再狹窄探討新的治療途徑.方法 建立慢性增生性膽管炎的實驗動物模型,膽總管內註入0.5 ml的PCNA shRNA,探討其對PC的過度增殖行為和成石潛力的影響.結果 PCNA shRNA可通過抑製PCNA和Procollagen Ⅲ mRNA和蛋白的錶達,有效抑製膽道黏膜上皮、黏膜下腺體和膠原纖維的過度增殖,有望達到控製或逆轉慢性增生性膽管炎及其所繼髮的膽道狹窄的目的 .此外,該治療還可抑製黏蛋白基因Mucin 5AC的激活和錶達,有助于降低PC的成石潛力.結論 PCNA shRNA治療可能在達到控製或逆轉慢性增生性膽管炎及其所繼髮的膽道狹窄的目的 同時,亦有助于降低病變膽管的成石潛力.
목적 근년적연구증실담결석적술후고복발솔화담도재협착솔균여술후유류적만성증생성담관염(PC)밀절상관,감우PC시일충과도증식질병,본연구의탐토응용PCNA shRNA억제PC적과도증식행위화성석잠력적가행성화료효,이기위예방결석적술후복발화담도재협착탐토신적치료도경.방법 건립만성증생성담관염적실험동물모형,담총관내주입0.5 ml적PCNA shRNA,탐토기대PC적과도증식행위화성석잠력적영향.결과 PCNA shRNA가통과억제PCNA화Procollagen Ⅲ mRNA화단백적표체,유효억제담도점막상피、점막하선체화효원섬유적과도증식,유망체도공제혹역전만성증생성담관염급기소계발적담도협착적목적 .차외,해치료환가억제점단백기인Mucin 5AC적격활화표체,유조우강저PC적성석잠력.결론 PCNA shRNA치료가능재체도공제혹역전만성증생성담관염급기소계발적담도협착적목적 동시,역유조우강저병변담관적성석잠력.
Objective In recent years, high stone recurrence and biliary restenosis rates in hepatolithiasis patients have been confirmed to be closely related to chronic proliferative cholangitis(PC).Since PC is a kind of chronic proliferative disease, we designed this study to investigate the feasibility and effectiveness of applying PCNA shRNA to inhibit the hyperplasia and lithogenic potentiality of PC and to explore a new treatment approach for the prevention of stone recurrence and biliary restenosis.Methods The common bile duct of a PC animal model was given an intralumenal administration of 0.5 ml of PCNA shRNA to investigate its influence on PC.Results The PCNA shRNA could efficiently inhibit the hyperplasia of the biliary epithelium, submucosal gland, and collagen fiber by inhibiting mRNA or protein expressions of PCNA and Procollagen Ⅲ, thus showing promise to control or reverse PC and its secondary biliary stricture.In addition, it could inhibit the lithogenic potentiality of PC by inhibiting the expression of mucin 5AC.Conclusion PCNA shRNA treatment might achieve the effect of controlling or reversing the PC and its subsequent biliary stricture and concurrently assist in preventing the recurrence of intrahepatic stone.
