CAJ | 학술논문

目的观察stathmin基因和蛋白质在喉鳞状细胞癌(简称鳞癌)中的表达情况,探讨stathmin基因与喉鳞癌生物学行为的关系及临床意义,进一步认识喉鳞癌发生和发展的分子机制.方法选取2005年3月至2006年4月的38例喉鳞癌患者,术中从肿瘤中心部位取癌组织(实验组),在肿瘤边缘外1.0cm部位取癌旁正常组织(对照组).应用半定量逆转录-聚合酶链反应(RT-PCR)分析38例患者的喉鳞癌组织和癌旁正常组织中stathmin基因mRNA的表达水平,冰冻切片免疫组织化学方法检测喉鳞癌组织和癌旁正常组织中stathmin蛋白的表达情况.结果 RT-PCR结果显示,stathmin基因mRNA在38例喉癌患者的喉癌组织和癌旁正常组织均呈阳性表达;经半定量分析,stathmin mRNA在喉癌组织中的表达水平明显高于在癌旁正常组织的表达水平(t=9.655,P＜0.05).免疫组织化学发现,stathmin蛋白在26例(26/38,68.4%)患者的喉癌组织呈阳性表达,在13例(13/38,34.2%)患者的癌旁正常组织呈弱阳性表达;stathmin蛋白在喉癌组织中的阳性表达率明显高于在癌旁正常组织的阳性表达率(x2=8.901,P＜0.05).同时发现,stathmin mRNA的表达水平和stathmin蛋白的阳性表达率在Ⅲ期和Ⅳ期喉癌组明显高于Ⅰ期和Ⅱ期病例组(t=6.284,x2=5.810,P＜0.05),有颈部淋巴转移组明显高于无转移组(t=9.350,x2=6.923,P＜0.05).结论 stathmin基因和蛋白在喉鳞癌中的表达明显高于癌旁正常组织,在中晚期喉鳞癌中的表达明显高于早期喉鳞癌,并且与颈淋巴转移有关.stathmin基因与喉鳞癌的发生和发展均有密切关系,并可能与喉癌的预后有关.
목적 관찰stathmin기인화단백질재후린상세포암(간칭린암)중적표체정황,탐토stathmin기인여후린암생물학행위적관계급림상의의,진일보인식후린암발생화발전적분자궤제.방법선취2005년3월지2006년4월적38례후린암환자,술중종종류중심부위취암조직(실험조),재종류변연외1.0cm부위취암방정상조직(대조조).응용반정량역전록-취합매련반응(RT-PCR)분석38례환자적후린암조직화암방정상조직중stathmin기인mRNA적표체수평,빙동절편면역조직화학방법검측후린암조직화암방정상조직중stathmin단백적표체정황.결과 RT-PCR결과현시,stathmin기인mRNA재38례후암환자적후암조직화암방정상조직균정양성표체;경반정량분석,stathmin mRNA재후암조직중적표체수평명현고우재암방정상조직적표체수평(t=9.655,P＜0.05).면역조직화학발현,stathmin단백재26례(26/38,68.4%)환자적후암조직정양성표체,재13례(13/38,34.2%)환자적암방정상조직정약양성표체;stathmin단백재후암조직중적양성표체솔명현고우재암방정상조직적양성표체솔(x2=8.901,P＜0.05).동시발현,stathmin mRNA적표체수평화stathmin단백적양성표체솔재Ⅲ기화Ⅳ기후암조명현고우Ⅰ기화Ⅱ기병례조(t=6.284,x2=5.810,P＜0.05),유경부림파전이조명현고우무전이조(t=9.350,x2=6.923,P＜0.05).결론 stathmin기인화단백재후린암중적표체명현고우암방정상조직,재중만기후린암중적표체명현고우조기후린암,병차여경림파전이유관.stathmin기인여후린암적발생화발전균유밀절관계,병가능여후암적예후유관.
Objective To investigate the expressions of stathmin gene and its coding protein in laryngeal squamous cell carcinoma,and to explore the relationship between stathmin gene and the biological behaviors of laryngeal squamous cell carcinoma for understanding the tumorigenicity and development of laryngeal squamous cell carcinoma.Methods Laryngeal carcinoma tissues (studying group) in the tumors'center and laryngeal normal tissues (control group) parted from 1.0 cm of the safe borderline of the tumors were took from 38 patients with laryngeal squamous cell carcinoma while they were in operation.Semi-quantitative method of reverse transcriptase polymerase chain reaction (RT-PCR) was used to analyze the expression level of stathmin mRNA,and immunohistochemical staining (frozen section) was used to detect the expressions of stathmin protein,in laryngeal carcinoma tissues and laryngeal normal tissues of 38 cases,respectively.Results mRNA of stathmin gene was all positively expressed in laryngeal carcinoma tissues and in laryngeal normal tissues of 38 cases by RT-PCR.However,stathmin mRNA was obviously over-expressed in laryngeal carcinoma tissues than that in laryngeal normal tissues (t=9.655,P＜0.05).Immunohistochemical staining showed stathmin protein was positively expressed in laryngeal carcinoma tissues of 26 cases (26/38,68.4%),and mild-positively expressed in laryngeal normal tissues in 13 cases(13/38,34.2%).There was significant difference between the expression rate of stathmin protein inlaryngeal carcinoma tissues and in laryngeal normal tissues (x2=8.901,P＜0.05).Meanwhile,theexpression level of stathmin mRNA and the positive-expressed rate of stathmin protein in laryngeal carcinoma tissues of the advanced stage patients group(Ⅰ stage and Ⅳ stage) were significantly higher than these in laryngeal carcinoma tissues of Ⅰ and Ⅱ stage patients group (t=6.284,x2=5.810,P＜0.05),and they were also significantly higher in laryngeal carcinoma tissues of the patients group with cervical lymph node metastasis than in laryngeal carcinoma tissues of the patients group without cervical lymph node metastasis (t=9.350,x2=6.923,P＜0.05).Conclusions The expression levels of stathmin gene and protein were significantly higher in laryngeal squamous cell carcinoma than these in laryngeal normal tissues,the levels are also significantly higher in advanced stage patients group (Ⅲ stage and Ⅳ stage) than in the early stage patients group (Ⅰ and Ⅱ),and they are also related to the cervical lymph node metastasis of carcinoma.Stathmin gene may play an important role in the pathogenisis and development of laryngeal carcinoma and may be related to its prognosis.