CAJ | 학술논문

目的探讨中国北方地区人群人类白细胞抗原(HLA)-DRB1基因启动子区序列多态性与肺结核的相关性.方法北方汉族肺结核患者97例为肺结核组,北方汉族健康人62例为对照组,采用PCR-SSP法对2组的HLA-DRB1基因启动子区进行PCR扩增,启动子区扩增产物直接测序.结果肺结核组和对照组的HLA-DRB1基因启动子区序列完全一致;对照组启动子区序列与数据库检索的序列比较有3个突变点:-26位的T被C替代,-49位的C被G替代,-78位的C被G替代.结论中国北方地区人群HLA-DRB1基因启动子区序列具有多态性,但此多态性与肺结核的发病没有相关性.
목적 탐토중국북방지구인군인류백세포항원(HLA)-DRB1기인계동자구서렬다태성여폐결핵적상관성.방법 북방한족폐결핵환자97례위폐결핵조,북방한족건강인62례위대조조,채용PCR-SSP법대2조적HLA-DRB1기인계동자구진행PCR확증,계동자구확증산물직접측서.결과 폐결핵조화대조조적HLA-DRB1기인계동자구서렬완전일치;대조조계동자구서렬여수거고검색적서렬비교유3개돌변점:-26위적T피C체대,-49위적C피G체대,-78위적C피G체대.결론 중국북방지구인군HLA-DRB1기인계동자구서렬구유다태성,단차다태성여폐결핵적발병몰유상관성.
Objective To analyze the association between gene sequences of HLA-DRB1 promoter region and pulmonary tuberculosis among Chinese people.Methods PCR/SSP Was performed to amplify the promoter region of pulmonary tuberculosis group and control group,and the PCR production were used for PCR direct sequencing.Results The pulmonary tuberculosis group had the same HLA-DRB1 promoter sequences as control group.Compared with those published in nueleotide database in network,the promoter sequences of control group had three mutation points:T in position -26 was replaced by C,C in position -49 was replaced by G,C in position -78 was replaced by G.Conclusion The HLA-DRB1 promoter sequences of Chinese people had polymorphism,but the polymorphism had no association with the development of pulmonary tuberculosis.