中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
5期
570-572
,共3页
张成森%刘翠薇%潘旭东%马爱军%邢岩
張成森%劉翠薇%潘旭東%馬愛軍%邢巖
장성삼%류취미%반욱동%마애군%형암
脑梗死%亚甲基四氢叶酸还原酶%多态性
腦梗死%亞甲基四氫葉痠還原酶%多態性
뇌경사%아갑기사경협산환원매%다태성
Cerebral infarction%Methylenetetrahydrofolate reductase%Polymorphism
目的 研究N5,N10 -亚甲基四氢叶酸还原酶(MTHFR)基因多态性与青年脑梗死的关系.方法 将67例青年脑梗死患者作为病例组,同期71例健康体检者作为对照组,用聚合酶链反应-限制性内切酶片段长度多态性分析(PCR-RFLP)的方法来观察MTHFR 677和1298位点基因型.结果 MTHFR 677位点基因型病例组中TT型为20例,占29.8％；CT型32例,占47.8％；CC型15例,占22.4％；T等位基因频率为53.7％.对照组中TT型30例,占42.2％；CT型34例,占47.9％；CC型7例,占9.9％；T等位基因频率为66.2％.对照组中TT型及T等位基因频率明显高于病例组,2组差异有统计学意义(P＜0.05).MTHFR1298位点基因型病例组中AC型29例,占43.3％；AA型38例,占56.7％；C等位基因频率为21.6％.对照组中AC型14例,占19.7％,AA型57例,占80.3％,C等位基因为9.9％,病例组中AC型及C等位基因频率明显高于对照组,2组差异有统计学意义(P＜0.05).结论 本组人群中MTHFR基因677及1298位点多态性与青年脑梗死都有相关性.
目的 研究N5,N10 -亞甲基四氫葉痠還原酶(MTHFR)基因多態性與青年腦梗死的關繫.方法 將67例青年腦梗死患者作為病例組,同期71例健康體檢者作為對照組,用聚閤酶鏈反應-限製性內切酶片段長度多態性分析(PCR-RFLP)的方法來觀察MTHFR 677和1298位點基因型.結果 MTHFR 677位點基因型病例組中TT型為20例,佔29.8％；CT型32例,佔47.8％；CC型15例,佔22.4％；T等位基因頻率為53.7％.對照組中TT型30例,佔42.2％；CT型34例,佔47.9％；CC型7例,佔9.9％；T等位基因頻率為66.2％.對照組中TT型及T等位基因頻率明顯高于病例組,2組差異有統計學意義(P＜0.05).MTHFR1298位點基因型病例組中AC型29例,佔43.3％；AA型38例,佔56.7％；C等位基因頻率為21.6％.對照組中AC型14例,佔19.7％,AA型57例,佔80.3％,C等位基因為9.9％,病例組中AC型及C等位基因頻率明顯高于對照組,2組差異有統計學意義(P＜0.05).結論 本組人群中MTHFR基因677及1298位點多態性與青年腦梗死都有相關性.
목적 연구N5,N10 -아갑기사경협산환원매(MTHFR)기인다태성여청년뇌경사적관계.방법 장67례청년뇌경사환자작위병례조,동기71례건강체검자작위대조조,용취합매련반응-한제성내절매편단장도다태성분석(PCR-RFLP)적방법래관찰MTHFR 677화1298위점기인형.결과 MTHFR 677위점기인형병례조중TT형위20례,점29.8％；CT형32례,점47.8％；CC형15례,점22.4％；T등위기인빈솔위53.7％.대조조중TT형30례,점42.2％；CT형34례,점47.9％；CC형7례,점9.9％；T등위기인빈솔위66.2％.대조조중TT형급T등위기인빈솔명현고우병례조,2조차이유통계학의의(P＜0.05).MTHFR1298위점기인형병례조중AC형29례,점43.3％；AA형38례,점56.7％；C등위기인빈솔위21.6％.대조조중AC형14례,점19.7％,AA형57례,점80.3％,C등위기인위9.9％,병례조중AC형급C등위기인빈솔명현고우대조조,2조차이유통계학의의(P＜0.05).결론 본조인군중MTHFR기인677급1298위점다태성여청년뇌경사도유상관성.
Objective To investigate the relation between polymorphisms of N5,N10-methylenetetrahydrofolate reductase(MTHFR) gene and cerebral infarction in young adults.Methods Sixty-seven consecutive young adult cerebral infarction and 71 healthy subjects were enrolled.The polymorphisms of MTHFR gene were analyzed using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results The frequencies of the MTHFR 677genotype were as follows:TT 20 cases(29.8％ ) ;CT 32 cases(47.8％ ) ; CC 15 cases(22.4％ ) in stroke patients and TT 30 cases(42.2％ ) ;CT 24 cases(47.9％ ) ;CC 7 cases(9.9％ ) in normal subjects,respectively.The frequency of T alleles was significantly higher in normal controls than that in stroke patients. The frequencies of the MTHFR 1298 genotype were as follows:AC,43.3％; AA,56.7％ in stroke patients and AC,19.7％ ; AA,80.3％ among normal subjects,respectively.The frequency of C alleles was significantly higher among stroke patients than that among normal controls.Conclusion MTHFR 677 and 1298 polymorphisms are positively associated with risk of cerebral infarction in young adults.
