CAJ | 학술논문

目的确定富含亮氨酸重复序列免疫球蛋白2(LRIG2)是胶质瘤细胞系GL15中EGFR信号通路新的调控靶点.方法培养胶质瘤细胞系GL15细胞,经表皮生长因子(EGF)100μg/L,AG1478 10 μmol/L,放线菌酮(CHX)10 mg/L体外干预后,逆转录-聚合酶链反应(RT-PCR)和Western blot测定LRIG2 mRNA和蛋白表达的时间效应变化.结果随着EGF作用时间的延长,LRIG2 mRNA先上升后恢复至正常水平.EGF作用后,表皮生长因子受体(EGFR),磷酸化EG-FR(pEGFR)均先上升后下降,LRIG2蛋白先下降,在30min时上升,120min下降至原水平的50%.CHX作用1.5 h后,再加入EGF刺激,可见LRIG2蛋白60 min降至原水平的50%.而AG1478作用1 h后,再用EGF刺激,EGFR未受明显影响,pEGFR被明显抑制,LRIG2蛋白水平变化不明显.结论 EGFR活化后可导致LRIG2 mRNA表达上升,LRIG2蛋白合成增加.
목적 학정부함량안산중복서렬면역구단백2(LRIG2)시효질류세포계GL15중EGFR신호통로신적조공파점.방법 배양효질류세포계GL15세포,경표피생장인자(EGF)100μg/L,AG1478 10 μmol/L,방선균동(CHX)10 mg/L체외간예후,역전록-취합매련반응(RT-PCR)화Western blot측정LRIG2 mRNA화단백표체적시간효응변화.결과 수착EGF작용시간적연장,LRIG2 mRNA선상승후회복지정상수평.EGF작용후,표피생장인자수체(EGFR),린산화EG-FR(pEGFR)균선상승후하강,LRIG2단백선하강,재30min시상승,120min하강지원수평적50%.CHX작용1.5 h후,재가입EGF자격,가견LRIG2단백60 min강지원수평적50%.이AG1478작용1 h후,재용EGF자격,EGFR미수명현영향,pEGFR피명현억제,LRIG2단백수평변화불명현.결론 EGFR활화후가도치LRIG2 mRNA표체상승,LRIG2단백합성증가.
Objective To confirm that LRIG2 is the new regulatory target of epidermal growth factor receptor (EGFR) signaling network in human glioma cell line GL15. Methods After the human glioma cell line GL15 was exposed to designed concentrations of EGF 100 μg/L,AG1478 10 μmol/L and Cycloheximide 10 mg/L in vitro, changes of mRNA and protein levels were measured by reverse transcriptional-polymerase chain reaction (RT-PCR) and Western blotting. Results The levels of LRIG2 mRNA and protein in GL15 cells were increased after EGF stimulation for 15 min and 30 min respectively. After Cycloheximide and AG1478 were added into GL15 before the exposure of EGF at different times,the protein levels of LRIG2 had no significant change. Conclusion The activation of EGFR can result in the expression of LRIG2 mRNA and protein.