CAJ | 학술논문

为评估小鼠巨噬细胞吞噬死亡细胞时胞质内游离钙离子的变化.实验使用Fluo-3标记巨噬细胞内钙离子和碘化丙碇对死亡细胞核染色,观察吞噬过程中细胞内钙离子的变化和显示巨噬细胞的吞噬功能,检测含死亡细胞的巨噬细胞内荧光密度图像.利用激光扫描共聚焦显微镜检测钙离子的释放.在缺钙的溶液中,可见巨噬细胞接触死细胞时细胞内钙离子快速地聚集和增高.在吞噬体形成时,巨噬细胞内钙离子上升到较高的水平.快速上升后,当吞噬小泡消化时,细胞内游离钙下降,随后钙离子恢复到低水平.研究显示伴随着吞噬小泡中红色荧光的死细胞的出现和消失,巨噬细胞内出现一系列钙离子变化的图像.提示巨噬细胞内钙离子改变在细胞吞噬作用中具有一定的作用.
위평고소서거서세포탄서사망세포시포질내유리개리자적변화.실험사용Fluo-3표기거서세포내개리자화전화병정대사망세포핵염색,관찰탄서과정중세포내개리자적변화화현시거서세포적탄서공능,검측함사망세포적거서세포내형광밀도도상.이용격광소묘공취초현미경검측개리자적석방.재결개적용액중,가견거서세포접촉사세포시세포내개리자쾌속지취집화증고.재탄서체형성시,거서세포내개리자상승도교고적수평.쾌속상승후,당탄서소포소화시,세포내유리개하강,수후개리자회복도저수평.연구현시반수착탄서소포중홍색형광적사세포적출현화소실,거서세포내출현일계렬개리자변화적도상.제시거서세포내개리자개변재세포탄서작용중구유일정적작용.
Cytosolic free calciu, m ( [ Ca2 + ] i ) change was assessed in single adherent mouse macrophages during phagocytosis for dead cells. To determine visualizing Ca2+ change during phagocytosis and indirectly show phagocytic function of macrophages to dead cells, intracellular [ Ca2 + ] i of macrophages were loaded with the fluorescent dye Fluo3/AM and dead cells were labeled with Propidium Iodide ( PI ) , we examined images pattern of the fluorescence intensity in macrophages of containing dead cells. A discharge of calcium was verified by confocal scanning laser microscopy ( CSLM ). In lacking Ca2 + medium, upon contact with dead cells, rapid assemble and increase of[ Ca2 + ] i was observed in macrophage. Intracellular [ Ca2 + ] i rose to a higher level during initial phagosome formation. After a rapid rise, free Ca2 + was decreased in digestion of phagocytotic vesicle; there-after the [ Ca2 + ] i returned to the baseline. Our study directly indicates that accompany presenting and disappearing of dead cell of red fluorescence in the phagosomal vesicle, a series of images pattern of [ Ca2 + ] i change can be really exhibited in macrophages. It is suggested that[ Ca2 + ] i fluctuation has a certainty in macrophages during the phagocytosis