生命科学研究
生命科學研究
생명과학연구
LIFE SCIENCE RESEARCH
2007年
2期
105-109
,共5页
喜温硫杆菌%16S rRNA%分离%特性%重金属
喜溫硫桿菌%16S rRNA%分離%特性%重金屬
희온류간균%16S rRNA%분리%특성%중금속
A cidithiobacillus caldus%16S rRNA%isolation%characterization%heavy metals
从江西德兴分离得到一株嗜酸硫氧化杆菌DX-2,采用双层固体培养基进行分离纯化,对分离菌株进行了形态、生理生化特性研究及16S rRNA序列分析.该菌株为革兰氏阴性细菌,短杆状,菌体大小(0.4～0.5)μm×(1～2)μm,化能自养,可利用硫磺和硫代硫酸盐为能源生长,不能利用亚铁进行生长.以16S rRNA序列同源性为基础构建了相关种属在内的系统发育树,结果表明,DX-2与喜温硫杆菌(Acidithiobacillus caldus)处于同一进化树分支中,相似性达99%以上.考察了不同重金属离子对DX-2的生长的影响.
從江西德興分離得到一株嗜痠硫氧化桿菌DX-2,採用雙層固體培養基進行分離純化,對分離菌株進行瞭形態、生理生化特性研究及16S rRNA序列分析.該菌株為革蘭氏陰性細菌,短桿狀,菌體大小(0.4～0.5)μm×(1～2)μm,化能自養,可利用硫磺和硫代硫痠鹽為能源生長,不能利用亞鐵進行生長.以16S rRNA序列同源性為基礎構建瞭相關種屬在內的繫統髮育樹,結果錶明,DX-2與喜溫硫桿菌(Acidithiobacillus caldus)處于同一進化樹分支中,相似性達99%以上.攷察瞭不同重金屬離子對DX-2的生長的影響.
종강서덕흥분리득도일주기산류양화간균DX-2,채용쌍층고체배양기진행분리순화,대분리균주진행료형태、생리생화특성연구급16S rRNA서렬분석.해균주위혁란씨음성세균,단간상,균체대소(0.4～0.5)μm×(1～2)μm,화능자양,가이용류광화류대류산염위능원생장,불능이용아철진행생장.이16S rRNA서렬동원성위기출구건료상관충속재내적계통발육수,결과표명,DX-2여희온류간균(Acidithiobacillus caldus)처우동일진화수분지중,상사성체99%이상.고찰료불동중금속리자대DX-2적생장적영향.
An acidophilic sulfur-oxidizing bacterium DX-2 was isolated from Dexing area, Jiangxi province in China using enrichment medium and then purified on overlaid solid medium. The morphological, biochemical and physiological characters of DX-2 were studied. The cells of the strain DX-2 were Gram negative and rod-shaped (0.4～0.5) μm by (1～2) μm. The strain was capable of growth by using elemental sulfur or sodium thiosulfate as the sole energy sources. The strain was unable to oxidize ferrous iron. A phylogenetic tree was constructed by comparing published 16S rRNA sequences of the relative bacteria species. In the phylogenetic tree, DX-2 was the closest relative to Acidithiobacillus caldus with more than 99% sequence similarity. The effect of different heavy metals (Cu2+, Cr3+, Cd2+, Sn2+, Pb2+) on growth inhibition of DX-2was investigated.
