CAJ | 학술논문

目的探讨脑缺血大鼠皮质抗氧化蛋白Ⅵ(peroxiredoxin Ⅵ)的表达以及"醒脑开窍"针刺法的干预作用.方法采用大鼠大脑中动脉阻塞(MCAO)模型,通过western blotting方法 ,检测醒脑开窍针刺组、对照组(传统针刺组)、模型组,假手术组Peroxiredoxin Ⅵ的表达.结果 6h时,醒脑开窍组与模型组比较表达明显升高(P<0.05);24h时,醒脑开窍组和对照组与模型组比较都有显著的升高(P<0.05).结论结果提示"醒脑开窍"针刺法能够提高大鼠脑缺血后Peroxiredoxin Ⅵ的表达,在缺血早期效果优于传统针刺法,对提高大鼠脑缺血后的抗氧化能力有积极意义.
목적 탐토뇌결혈대서피질항양화단백Ⅵ(peroxiredoxin Ⅵ)적표체이급"성뇌개규"침자법적간예작용.방법 채용대서대뇌중동맥조새(MCAO)모형,통과western blotting방법 ,검측성뇌개규침자조、대조조(전통침자조)、모형조,가수술조Peroxiredoxin Ⅵ적표체.결과 6h시,성뇌개규조여모형조비교표체명현승고(P<0.05);24h시,성뇌개규조화대조조여모형조비교도유현저적승고(P<0.05).결론 결과 제시"성뇌개규"침자법능구제고대서뇌결혈후Peroxiredoxin Ⅵ적표체,재결혈조기효과우우전통침자법,대제고대서뇌결혈후적항양화능력유적겁의의.
Objective To investigate and observe the expression of Peroxiredoxin Ⅵ in iechemia cortex of rats and the intervention effect of Xingnao Kaiqiao Acupuncture(XKA) therapy. Methods Through western blotting,we detected the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group , comparison group, model group and sham operation group in middle cerebral artery occlusion (MCAO)model of rats. Results At 6h, the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group , comparison group and sham operation group was increased than that in moddle group,of which Xingnao Kaiqiao group increased obviously(P < 0.05). At 24h, as compared with modlegroup, the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group and comparison group increased significantly (P < 0.05). Conclusion Xingnao Kaiqiao Acupuncture therapy could raise the expression of Peroxiredoxin Ⅵ in focal cerebral ischemia of rats, and the effect is better that of traditional acupuncture therapy in the earlier period.It is benefit to elevating the ability of antioxygen of focal cerebral ischemia of rats.