CAJ | 학술논문

采用生物测定的方法,以黄瓜枯萎病菌(Fusarium oxysporium Schl.)等蔬菜病原真菌为供试菌种,系统地研究了飞廉提取液对不同提取溶剂、不同提取方法、不同提取部位的蔬菜病原菌抑制作用.结果表明,不同提取溶剂的提取收率、抑菌效果存在明显差异.乙醇提取物抑菌活性最好,提取收率为5.83%.快速溶剂萃取法简单、快速、需要提取溶剂量少、提取收率大、抑菌效果好.飞廉各部位均有抑菌活性,根、茎叶、花EC50分别为3.65、1.45和2.03 mg·mL-1,具有开发研究价值.
채용생물측정적방법,이황과고위병균(Fusarium oxysporium Schl.)등소채병원진균위공시균충,계통지연구료비렴제취액대불동제취용제、불동제취방법、불동제취부위적소채병원균억제작용.결과표명,불동제취용제적제취수솔、억균효과존재명현차이.을순제취물억균활성최호,제취수솔위5.83%.쾌속용제췌취법간단、쾌속、수요제취용제량소、제취수솔대、억균효과호.비렴각부위균유억균활성,근、경협、화EC50분별위3.65、1.45화2.03 mg·mL-1,구유개발연구개치.
With bioassay, the research studied inhibition activities of the extract from Carduus crispus L. Of different solvents, extract methods and extract parts on Fusarium oxysporium Schl. And vegetable pathogen. The studies showed that it existed significant difference of extract yeild and inhibition activities. The best solvent was alcohol and its extract yeild was 5.83%. Accelerated solvent extraction was simple, fast, needing less solvent, extract yeild high and bactedostasis well. Extract from every part of Carduus crispus L. Had inhibition activities. The EC50 of root, stem and flower was 3.65, 1.45 and 2.03 mg·mL-1, so it was worth developing and studying.