CAJ | 학술논문

目的:探讨腺样囊性癌肿瘤抗原负载的树突状细胞通过淋巴细胞介导的免疫反应,体外杀伤腺样囊性癌细胞的细胞毒性效应.方法:外周血单核细胞在GM-CSF + IL-4 的诱导下体外培养,用肿瘤细胞抗原冲击后,流式细胞仪检测树突状细胞抗原负载前后CD1a、CD83表达量的变化.MTT比色法检测同种异体的混合淋巴细胞反应和诱导细胞毒淋巴细胞CTL杀伤肿瘤细胞.结果:凋亡肿瘤抗原刺激后,CD83 表达增加﹙P<0.01﹚,而CD1a表达量下调﹙P<0.05).负荷肿瘤抗原树突状细胞体外诱导出明显的细胞不良反应,并刺激同种T淋巴细胞增殖.结论:GM-CSF + IL-4 诱导的单核细胞来源的树突状细胞,能在体外摄取肿瘤抗原而进一步成熟,通过激活淋巴细胞杀伤癌细胞.
목적:탐토선양낭성암종류항원부재적수돌상세포통과림파세포개도적면역반응,체외살상선양낭성암세포적세포독성효응.방법:외주혈단핵세포재GM-CSF + IL-4 적유도하체외배양,용종류세포항원충격후,류식세포의검측수돌상세포항원부재전후CD1a、CD83표체량적변화.MTT비색법검측동충이체적혼합림파세포반응화유도세포독림파세포CTL살상종류세포.결과:조망종류항원자격후,CD83 표체증가﹙P<0.01﹚,이CD1a표체량하조﹙P<0.05).부하종류항원수돌상세포체외유도출명현적세포불량반응,병자격동충T림파세포증식.결론:GM-CSF + IL-4 유도적단핵세포래원적수돌상세포,능재체외섭취종류항원이진일보성숙,통과격활림파세포살상암세포.
Objective:To investigate the effect of T lymphocytes activated by dendritic cells loaded with salivary adenoid cystic carcinoma antigen on salivary adenoid cystic carcinoma (SACC)cells. Methods: Human peripheral blood monocots were cultured in RPMI 1640 medium containing 10 % FBS with GM-CSF + IL-4,and continuously incubated with SACC cell lysate made by loaded with heat-shocked antigen and freeze-thaw antigen on the day of DC culture.The expression of DC phenotypes CD1a and CD83 were evaluated by flow cytometry before and after the pulse.DC function was evaluated by their ability to induce proliferation of allogeneic T cells,the cytotoxicity of cytotoxic T lymphocyte( CTL ) activated by DCs loaded with heat-shocked antigen and freeze-thaw antigen evaluated by MTT method. Results: The expression of CD83 increased after tumor antigen stimulation and CD1a was down-regulated.The cytotoxicity against SACC cells was significantly induced by DC loaded with tumour antigen,and lymphocytes proliferation stimulated. Conclusion:DC generated from monocytes in the presence of GM-CSF and IL-4 can develop to become mature after tumor antigen pulse.CTL activated by DC loaded with freeze-thaw antigen has effective cytotoxicity against SACC cells in vitro.