CAJ | 학술논문

背景与目的:观察苯和甲醛对胚胎发育的联合毒性作用,并探讨联合作用类型.材料与方法:采用体外全胚胎培养模型,按照3× 4析因设计的要求共分12组.将8.5日龄昆明种小鼠胚胎与含不同浓度苯和甲醛的即刻离心血清共培养48 h,观察苯和甲醛对小鼠胚胎生长发育和组织器官形态分化的影响及其对胚胎卵黄囊结构的损伤作用,并通过直观分析和方差分析判断苯与甲醛联合作用的类型.结果:空白和溶剂对照组培养胚胎与体内同龄胚胎的发育基本一致(P>0.05);苯和甲醛对小鼠胚胎发育均具有抑制作用,生长发育和器官分化各指标均低于对照组(P<0.01),且呈剂量一效应关系,较高浓度时可诱发胚胎畸型,甚至死亡,其主要表现为头部异常、心脏发育异常、肢芽小、眼、耳、鳃弓和体位异常等;胚胎卵黄囊细胞结构紊乱、排列不规则,变性、肿胀,胞浆出现空泡.苯与甲醛对胚胎的毒作用表现为协同效应.结论:苯和甲醛对小鼠胚胎具有直接的胚胎发育毒性和致畸性,二者表现为协同毒性效应.
배경여목적:관찰분화갑철대배태발육적연합독성작용,병탐토연합작용류형.재료여방법:채용체외전배태배양모형,안조3× 4석인설계적요구공분12조.장8.5일령곤명충소서배태여함불동농도분화갑철적즉각리심혈청공배양48 h,관찰분화갑철대소서배태생장발육화조직기관형태분화적영향급기대배태란황낭결구적손상작용,병통과직관분석화방차분석판단분여갑철연합작용적류형.결과:공백화용제대조조배양배태여체내동령배태적발육기본일치(P>0.05);분화갑철대소서배태발육균구유억제작용,생장발육화기관분화각지표균저우대조조(P<0.01),차정제량일효응관계,교고농도시가유발배태기형,심지사망,기주요표현위두부이상、심장발육이상、지아소、안、이、새궁화체위이상등;배태란황낭세포결구문란、배렬불규칙,변성、종창,포장출현공포.분여갑철대배태적독작용표현위협동효응.결론:분화갑철대소서배태구유직접적배태발육독성화치기성,이자표현위협동독성효응.
BACKGROUND AND AIM: To explore the combined toxic effects of benzene and formaldehyde on mouse embryos in vitro. MATERIALS AND METHODS: Whole embryo culture system was adopted. According to 3 × 4 factorial design, 12 groups were set up. The 8.5-day isolated mouse embryos were cocultured for 48 hours in freshly centrifuged serum with different concentrations of benzene and formaldehyde. The growth, development and morphodifferentiation of tissues and organs of mouse embryos were studied. The type of combined toxic effects of benzene and formaldehyde was analyzed by ANOVA and direct-viewing analysis. RESULTS: There were no statistically significant differences in embryonic growth and development among control groups in vitro and in vivo group (P>0.05) . Benzene and formaldehyde had obvious toxic effect on growth and development in embryos in a dose-dependent manner every index of growth and development, morphodifferentiantion of tissues and organs demonstrated significant differences compared with the solvent control (P<0.05) . With the increasing concentrations of benzene and formaldehyde, the incidence of malformation and death of embryo increased. Structure, arrangement and cell polarity of VYS endodermis cells were influenced. When benzene was combined with formaldehyde, the severity of the toxic effects on the embryo was stronger than the addition of the two agents alone. CONCLUSION: Benzene and formaldehyde could cause synergistic developmental toxicity and teratogenicity to embryo in vitro.