CAJ | 학술논문

目的探讨七叶皂苷钠对油酸诱导大鼠急性肺损伤模型的干预作用.方法 ♂ SD大鼠54只随机分为5组,分别为正常对照组、注射七叶(非油酸造模)组、油酸造模组、甲基强的松龙组和七叶皂苷钠组.采用鼠尾静脉缓慢注射油酸(OA,0.1 ml·kg~(-1))复制大鼠急性肺损伤模型,模型复制成功后按相应措施处理观察并取血取材检测相关指标.观察检测指标有肺组织形态学、肺湿/干重(W/D)、光镜下半定量肺损伤评分(IQA)、血气分析动脉血氧分压(PaO_2)、血浆及肺组织匀浆超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量.结果 ①肺组织形态学:七叶皂苷钠组及甲基强的松龙组肺表面充血程度较油酸组明显减轻,气管内粉红色分泌物溢出减少;光镜下,七叶皂苷钠组及甲基强的松龙组较油酸造模组肺泡腔内炎症细胞渗出减轻; ②肺湿/干重(W/D):油酸造模组W/D较空白对照组明显升高,七叶皂苷钠组及甲基强的松龙组W/D较油酸造模组明显降低;③光镜下半定量肺损伤评分(IQA):油酸造模组IQA较空白对照组明显升高,七叶皂苷钠组及甲基强的松龙组IQA较油酸造模组明显降低;④血气分析动脉血氧分压(PaO_2):油酸造模组PaO_2较空白对照组明显降低,七叶皂苷钠组及甲基强的松龙组PaO_2较油酸造模组升高;⑤血浆及肺组织匀浆超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量:油酸造模组血浆及肺组织SOD活性较空白对照组明显降低,七叶皂苷钠组及甲基强的松龙组血浆和肺组织SOD活性较油酸造模组明显升高;油酸造模组血浆及肺组织MDA含量较空白对照组明显升高,七叶皂苷钠组及甲基强的松龙组血浆和肺组织MDA含量较油酸造模组明显降低.结论七叶皂苷钠通过对急性肺损伤大鼠氧化应急的调节作用,以改善W/D、IQA、PaO_2,从而为临床治疗急性肺损伤提供一个可能的途径.
목적 탐토칠협조감납대유산유도대서급성폐손상모형적간예작용.방법 ♂ SD대서54지수궤분위5조,분별위정상대조조、주사칠협(비유산조모)조、유산조모조、갑기강적송룡조화칠협조감납조.채용서미정맥완만주사유산(OA,0.1 ml·kg~(-1))복제대서급성폐손상모형,모형복제성공후안상응조시처리관찰병취혈취재검측상관지표.관찰검측지표유폐조직형태학、폐습/간중(W/D)、광경하반정량폐손상평분(IQA)、혈기분석동맥혈양분압(PaO_2)、혈장급폐조직균장초양화물기화매(SOD)활성급병이철(MDA)함량.결과 ①폐조직형태학:칠협조감납조급갑기강적송룡조폐표면충혈정도교유산조명현감경,기관내분홍색분비물일출감소;광경하,칠협조감납조급갑기강적송룡조교유산조모조폐포강내염증세포삼출감경; ②폐습/간중(W/D):유산조모조W/D교공백대조조명현승고,칠협조감납조급갑기강적송룡조W/D교유산조모조명현강저;③광경하반정량폐손상평분(IQA):유산조모조IQA교공백대조조명현승고,칠협조감납조급갑기강적송룡조IQA교유산조모조명현강저;④혈기분석동맥혈양분압(PaO_2):유산조모조PaO_2교공백대조조명현강저,칠협조감납조급갑기강적송룡조PaO_2교유산조모조승고;⑤혈장급폐조직균장초양화물기화매(SOD)활성급병이철(MDA)함량:유산조모조혈장급폐조직SOD활성교공백대조조명현강저,칠협조감납조급갑기강적송룡조혈장화폐조직SOD활성교유산조모조명현승고;유산조모조혈장급폐조직MDA함량교공백대조조명현승고,칠협조감납조급갑기강적송룡조혈장화폐조직MDA함량교유산조모조명현강저.결론 칠협조감납통과대급성폐손상대서양화응급적조절작용,이개선W/D、IQA、PaO_2,종이위림상치료급성폐손상제공일개가능적도경.
Aim To observe the intervention effects of sodium aescinate on acute lung injury model of rats induced by oleate. Methods Fifty four male SD rats were randomly divided into five groups: normal control group, sodium aescinate control group (without oleate) , oleate model control group,medrol interventional group and sodium aescinate interventional group. Acute lung injury models of rats were made by injecting oleate (OA, 0. 1 ml · kg~(-1) ) through caudal veins, and then rats were observed and killed to detect correlated in-dice. The observation indice were the histomorphology of lung, the wet and dry weights of lung ( W/D), score of injury of lung under light microscope (IQA ) , partial pressure of oxygen in artery ( PaO_2) , the levels of SOD and MDA in blood plasma and lung tissue. Results ① Histomorphology of lung: Lung surface hyperemia relieved obviously and pink secretion from trachea of rats in sodium aescinate interventional group and medrol interventioal group decreased significantly compared with oleate model control group. Under light microscope , compared with oleate model control group, effusion of inflammatory cells in alveolar space of rats in sodium aescinate interventional group and medrol interventional group decreased. ② The wet and dry weights of lung ( W/D ) ; W/D of rats in oleate control model group increased obviously compared with those in normal control group, W/D of rats in sodium aescinate interventional group and medrol interventional group decreased obviously compared with those in oleate model control group. ③ Score of injury of lungs under light microscope (IQA) ; IQA of rats in oleate model control group advanced obviously compared with that in normal control group. IQA of rats in sodium aescinate interventional group and medrol interventional group lowered significantly compared with that in oleate model control group.④ Partial pressure of oxygen in artery (PaO_2) : PaO_2 of rats in oleate model control group lowered significantly compared with that in normal control group. PaO_2 of rats in sodium aescinate interventional group and medrol interventional group improved significantly compared with that in oleate model control group. ⑤ The levels of SOD and MDA in blood plasma and lung tissue:The levels of SOD in plasma and lung tissue of rats in oleate model control group lowered significantly compared with those in normal control group. SOD in plasma and lung tissue of rats in sodium aescinate in-terventional group and medrol interventional group increased significantly compared with that in oleate model control group. The levels of MDA in plasma and lung tissue of rats in oleate model control group lowered significantly compared with those in normal control group. MDA in plasma and lung tissue of rats in sodium aescinate interventional group and medrol interventional group increased significantly compared with that in oleate model control group. Conclusion Sodium aescinate can improve W/D, IQA and PaO_2 by adjusting oxidization of the acute lung injury model of rats, which may provide a possible path for treating acute lung injury in clinical practice.