林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2010年
1期
142-146
,共5页
宿红艳%王磊%王仲礼%冯培勇%张晔
宿紅豔%王磊%王仲禮%馮培勇%張曄
숙홍염%왕뢰%왕중례%풍배용%장엽
杨树%MYB基因%克隆%表达分析%雄花序
楊樹%MYB基因%剋隆%錶達分析%雄花序
양수%MYB기인%극륭%표체분석%웅화서
poplar%MYB gene%cloning%expression analysis%male inflorescence
An R2R3 MYB gene,PeMYBL1,was isolated from male inflorescence of Populus×euramericana by homologous cloning combined with in silico cloning techniques.The full length of PeMYBL1 cDNA was 1 094 bp encoding 276 amino acids.The deduced amino acid sequence contained two conserved MYB domains near the N-terminus,a conserved E1 motif and an acidic Ser/Thr rich region toward its C terminus.Phylogenetic analysis revealed that PeMYBL1 was clustered with AtMYB85 from Arabidopsis thaliana,ZmMYBL1 from Zea mays,OsMYB15 from Oryza sativa and ODORANT1 from Petunia hybrida.Furthermore,expression analysis by RT-PCR showed that PeMYBL1 was expressed in root,stem,leaf,male and female infloresences,and abundantly accumulated in male inflorescences.The expression level of PeMYBL1 increased with the development of male inflorescences,indicating that PeMYBL1 is closely related to male flower development.
