CAJ | 학술논문

目的观察不同剂量中药单体复方(由白藜芦醇、枸杞多糖和槲皮素3种中药单体及L-肉碱组成)促进~(60)Co-γ致小鼠睾丸生精功能障碍的恢复作用,并探讨其最佳剂量.方法 72只雄性昆明小鼠随机分为5组,A组(n=12)正常喂饲,B、C、D、E组(各15只)小鼠均采用~(60)Co-γ 60y全身均匀照射I次,构造小鼠睾丸生精障碍模型,1周后C、D、E组分别用40、80、160mg/kg·d~(-1)浓度的中药单体复方混悬液灌胃35d,A组和B组小鼠用等量生理盐水灌胃.记录小鼠的一般状况、体征及体重变化,于灌胃结束24h后处死,测量小鼠体重、睾丸重量及睾丸指数;酶联免疫法测定血清FSH、LH、T和E_2水平并计算T/E_2比值;并对睾丸组织进行组织学观察.结果 D组(80mg/kg·d~(-1))小鼠体重、睾丸重量及睾丸指数均较B组有明显改善(p<0.05),接近A组(p>0.05),C组和E组虽有所改善但仍低于A组(P<0.05).C、D、E组小鼠血清生殖激素水平均有一定程度的恢复,但D组各生殖激素指标均接近A组(P>0.05).组织学观察见各给药组睾丸生精小管生精细胞层数、精子数目及c.kit阳性表达均高于B组,尤以D组(80mg/kg·d~(-1))恢复最为明显.结论由白藜芦醇、枸杞多糖、槲皮素和L.肉碱组成的中药单体复方制剂能够促进~(60)Co-γ致小鼠睾丸生精功能障碍后生精功能的恢复,且最佳剂量为80mg/kg·d~(-1) .
목적 관찰불동제량중약단체복방(유백려호순、구기다당화곡피소3충중약단체급L-육감조성)촉진~(60)Co-γ치소서고환생정공능장애적회복작용,병탐토기최가제량.방법 72지웅성곤명소서수궤분위5조,A조(n=12)정상위사,B、C、D、E조(각15지)소서균채용~(60)Co-γ 60y전신균균조사I차,구조소서고환생정장애모형,1주후C、D、E조분별용40、80、160mg/kg·d~(-1)농도적중약단체복방혼현액관위35d,A조화B조소서용등량생리염수관위.기록소서적일반상황、체정급체중변화,우관위결속24h후처사,측량소서체중、고환중량급고환지수;매련면역법측정혈청FSH、LH、T화E_2수평병계산T/E_2비치;병대고환조직진행조직학관찰.결과 D조(80mg/kg·d~(-1))소서체중、고환중량급고환지수균교B조유명현개선(p<0.05),접근A조(p>0.05),C조화E조수유소개선단잉저우A조(P<0.05).C、D、E조소서혈청생식격소수평균유일정정도적회복,단D조각생식격소지표균접근A조(P>0.05).조직학관찰견각급약조고환생정소관생정세포층수、정자수목급c.kit양성표체균고우B조,우이D조(80mg/kg·d~(-1))회복최위명현.결론 유백려호순、구기다당、곡피소화L.육감조성적중약단체복방제제능구촉진~(60)Co-γ치소서고환생정공능장애후생정공능적회복,차최가제량위80mg/kg·d~(-1) .
Objective To study the effect of Compound Chinese Herbal Monomer Recipe which were made of resveratrol,lycium barbarum polysaccharide(LBP),quercetin and L-camitine on spermatogenic disturbance in mice induced by~(60)Co.γ,and to explore the best effective dose.Methods Seventy-two male Kunming mice were randomly divided into five groups including Group A(normal,n=1 2),Group B,C,D and E with spermatogenic dysfunction estabilished by the irradiation of the~(60)Co-γ with 6 Gy(n+15 each).After a week,Group C,D and E received intragastric administration of Compound Chinese Herbal Monomer Recipe of different concentrations(40,80 and 160 mg/kg·d~(-1),respectively)for 35 days,and mice in other two groups were treated with physiological saline.Then the general condition,physical sign and body weight of mice were recorded, and 24 hours after finishing gavage,the weight of testes were measured and the testicular organ index were calculated;the levels of FSH,LH,T and E_2 determined by enzyme-linked immunosorbent assay and the ratio of T/E_2 were calculated;Histological observation of the testis tissues were done under the microscope.Results Compared with those of mice in Group B,the body weight,the testis weight and the testis index of mice in Group D(80 mg/kg·d~(-1))were improved obviously(P<0.05),similar with mice in Group A(P>0.05);Though the body weight、the testis weight and the testis index of mice in Group C and E were also improved.they were still lower than those of mice in Group A(P<0.05).The levels of the serum reproductive hormone of mice in Group C,D and E had some degree of recovery,but those of mice in Group D were close to that of normal(P>O.05).The layers of spermatogenic cells,the number of sperm and the positive expression of c-Kit in spermatogenic tubules of mice in Group C.D and E were superior to those of mice in Group B,especially in Group D(80 mg/kg·d~(-1).Conclusion Compound Chinese Herbal Monomer Recipe could improve effectively the spermatogenic dysfunction in mice induced by~(60)Co-γ.and the best dose of Compound Chinese Herbal Monomer Recipe was 80mg/kg·d~(-1).