中国医科大学学报
中國醫科大學學報
중국의과대학학보
JOURNAL OF CHINA MEDICAL UNIVERSITY
2010年
3期
209-211
,共3页
大肠癌%表面增强激光解吸电离%飞行时间%质谱技术%弱阳离子蛋白质芯片%标志物
大腸癌%錶麵增彊激光解吸電離%飛行時間%質譜技術%弱暘離子蛋白質芯片%標誌物
대장암%표면증강격광해흡전리%비행시간%질보기술%약양리자단백질심편%표지물
colorectal cancer%surface-enhanced laser desorption/ionization%time of flight%mass spectrometry%weak cation exchanger protein chip%marker
目的 采用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术检测大肠癌患者与健康对照者血清中蛋白的差异,为大肠癌的早期诊断提供理论依据.方法 应用SELDI-TOF-MS技术及弱阳离子蛋白质芯片(WCX2)检测36例健康成人(对照组)和36例大肠癌患者(大肠组)血清中蛋白含量.结果 在质荷比为2 000Da～30 000Da的范围内,两组样本检测到了对照组82个蛋白峰.其中3 604.240 Da蛋白峰大肠癌组明显高于对照组(P＜0.05),而12 861.368 Da的蛋白峰对照组明显高于大肠癌组(P＜0.05).利用这两个蛋白筛查大肠癌的特异度为86.11%(31/36),敏感度为77.78%(28,36),阳性预测值为87.5%.结论 质荷比为3 604.240 Da与12 861.368 Da的两个蛋白峰可以作为大肠癌早期诊断的备选标志物.
目的 採用錶麵增彊激光解吸電離飛行時間質譜(SELDI-TOF-MS)技術檢測大腸癌患者與健康對照者血清中蛋白的差異,為大腸癌的早期診斷提供理論依據.方法 應用SELDI-TOF-MS技術及弱暘離子蛋白質芯片(WCX2)檢測36例健康成人(對照組)和36例大腸癌患者(大腸組)血清中蛋白含量.結果 在質荷比為2 000Da～30 000Da的範圍內,兩組樣本檢測到瞭對照組82箇蛋白峰.其中3 604.240 Da蛋白峰大腸癌組明顯高于對照組(P＜0.05),而12 861.368 Da的蛋白峰對照組明顯高于大腸癌組(P＜0.05).利用這兩箇蛋白篩查大腸癌的特異度為86.11%(31/36),敏感度為77.78%(28,36),暘性預測值為87.5%.結論 質荷比為3 604.240 Da與12 861.368 Da的兩箇蛋白峰可以作為大腸癌早期診斷的備選標誌物.
목적 채용표면증강격광해흡전리비행시간질보(SELDI-TOF-MS)기술검측대장암환자여건강대조자혈청중단백적차이,위대장암적조기진단제공이론의거.방법 응용SELDI-TOF-MS기술급약양리자단백질심편(WCX2)검측36례건강성인(대조조)화36례대장암환자(대장조)혈청중단백함량.결과 재질하비위2 000Da～30 000Da적범위내,량조양본검측도료대조조82개단백봉.기중3 604.240 Da단백봉대장암조명현고우대조조(P＜0.05),이12 861.368 Da적단백봉대조조명현고우대장암조(P＜0.05).이용저량개단백사사대장암적특이도위86.11%(31/36),민감도위77.78%(28,36),양성예측치위87.5%.결론 질하비위3 604.240 Da여12 861.368 Da적량개단백봉가이작위대장암조기진단적비선표지물.
Objective To detect the specific serum protein of colorectal cancer by surface-enhanced laser desorption/ionization tune of flight mass spectrometry(SELDI-TOF-MS)and provide the foundation for early detection of colorectal cancer.Methods The levels of serum protein of 36 patients with colorectal cancer and 36 healthy control subjects were detected by weak cation exchanger protein chip(WCX2)chip and SELDI-TOF-MS.Results At the M/Z value range from 2 000 Da to 30 000 Da,82 protein peaks were found.The level of 3 604.240 Da protein was significantly higher in patients with colorectal cancer than in healthy control subjects,while the level of 12 861.368 Da protein was significantly higher in healthy control subjects than in patients with colorectal cancer(P＜0.05).The specificity,sensitivity,and positive predictive value of the detection of colorectal cancer using these 2 protien were 86.11%,77.78%,and 87.5%.respectively.Conclusion The 3 604.240 Da and 12 861.368 Da proteins can be used as the serum marker for early detection of colorectal cancer.
