CAJ | 학술논문

目的探讨不同游离脂肪酸(FFAs)对MDA-MB-231人乳腺癌细胞凋亡和迁移的影响.方法用50、200、500 umol/L三种不同浓度的油酸(0A)和棕榈酸(PA)分别刺激MDA-MB-231细胞,采用实时定量PCR分析细胞中PTEN的mRNA含量,蛋白免疫印迹法检测PTEN和Bcl-2的蛋白表达,原位末端标记法(TUNEL)观察细胞的凋亡情况,小室迁移实验测定细胞的迁移能力.结果OA使MDA-MB-231细胞中PTEN的mRNA和蛋白表达水平降低,Bcl-2蛋白表达升高(P均＜0.05)；PA则使PTEN的mRNA和蛋白表达水平上升,Bcl-2蛋白表达下降(P均＜0.05).显微镜下观察到PA刺激后的MDA-MB-231细胞凋亡增加.迁移实验显示OA和PA都能使乳腺癌细胞侵袭能力增强.结论PA可能通过上调PTEN的表达促进MDA-MB-231人乳腺癌细胞凋亡,但同时也增强细胞的侵袭能力.OA也可增强癌细胞侵袭能力.
목적탐토불동유리지방산(FFAs)대MDA-MB-231인유선암세포조망화천이적영향.방법용50、200、500 umol/L삼충불동농도적유산(0A)화종려산(PA)분별자격MDA-MB-231세포,채용실시정량PCR분석세포중PTEN적mRNA함량,단백면역인적법검측PTEN화Bcl-2적단백표체,원위말단표기법(TUNEL)관찰세포적조망정황,소실천이실험측정세포적천이능력.결과OA사MDA-MB-231세포중PTEN적mRNA화단백표체수평강저,Bcl-2단백표체승고(P균＜0.05)；PA칙사PTEN적mRNA화단백표체수평상승,Bcl-2단백표체하강(P균＜0.05).현미경하관찰도PA자격후적MDA-MB-231세포조망증가.천이실험현시OA화PA도능사유선암세포침습능력증강.결론PA가능통과상조PTEN적표체촉진MDA-MB-231인유선암세포조망,단동시야증강세포적침습능력.OA야가증강암세포침습능력.
Objective To observe the effects of free fatty aids (FFAs) on the apoptosis and migration of human breast cancer cells ( MDA-MB-231 ).Methods Breast cancer cells were exposed to oleic acid (OA) and palmitic acid (PA) with different concentrations (50,200,and 500 umol/L).Quantity RT-PCR was conducted to detect mRNA expression of PTEN,and Western blot to detect protein expression of PTEN and Bcl-2.Cell apoptosis was measured with TUNEL method,and migration measured with transwell method.Results The mRNA and protein expressions of PTEN in MDA-MB-231 cells were down-regulated after OA stimulation,but up-regulated after PA stimulation ( all P ＜ 0.05 ).The protein expression of Bcl-2 increased after OA stimulation and decreased after PA stimulation ( both P ＜ 0.05 ).PA increased the apoptosis,and both OA and PA enhanced the invasion of MDA-MB-231 cells.Conclusions PA may promote apoptosis of MDA-MB-231 cells through changing the expression of the tumor suppressor PTEN,but also increases the invasion of the cancer cells.The invasion of cancer cells could be enhanced by OA stimulation as well.