CAJ | 학술논문

目的评价乳腺癌七皮间质转化(EMT)与基底细胞样型乳腺癌(BLBC)的关系.方法依据免疫表型从458例浸润性乳腺癌中筛选出基底细胞样型、管腔A型、管腔B型和HER2过表达型共382例.免疫组织化学EnVision二步法检测FOXC-2、波形蛋白、Syndecan-1和E-cadherin在乳腺癌中表达,并分析其与BLBC的关系.结果 41例BLBC中癌细胞FOXC-2、波形蛋白和Syndecan-1的阳性率以及E-cadherin表达减弱率分别为34.1%(14/41)、43.9%(18/41)、87.7%(36/41)和63.4%(26/41).BLBC的FOXC-2和波形蛋白阳性率均高于其他亚型乳腺癌,其E-cadherin表达较其他亚型乳腺癌下降(P<0.01).BLBC肿瘤间质细胞Syndecan-1阳性率为41.5%(17/41),高于其他亚型乳腺癌(P=0.007).癌细胞FOXC-2表达与波形蛋白表达呈正相关(r=0.607,P<0.01).FOXC-2和波形蛋白阳性组腋窝淋巴结转移率分别为71.4%和66.7%,分别高于阴性组(P=0.002和P=0.001).结论本结果显示乳腺癌中EMT与BLBC关系最密切.EMT可能是造成BLBC与其他亚型乳腺癌生物学行为差异的蕈要原因.
목적 평개유선암칠피간질전화(EMT)여기저세포양형유선암(BLBC)적관계.방법 의거면역표형종458례침윤성유선암중사선출기저세포양형、관강A형、관강B형화HER2과표체형공382례.면역조직화학EnVision이보법검측FOXC-2、파형단백、Syndecan-1화E-cadherin재유선암중표체,병분석기여BLBC적관계.결과 41례BLBC중암세포FOXC-2、파형단백화Syndecan-1적양성솔이급E-cadherin표체감약솔분별위34.1%(14/41)、43.9%(18/41)、87.7%(36/41)화63.4%(26/41).BLBC적FOXC-2화파형단백양성솔균고우기타아형유선암,기E-cadherin표체교기타아형유선암하강(P<0.01).BLBC종류간질세포Syndecan-1양성솔위41.5%(17/41),고우기타아형유선암(P=0.007).암세포FOXC-2표체여파형단백표체정정상관(r=0.607,P<0.01).FOXC-2화파형단백양성조액와림파결전이솔분별위71.4%화66.7%,분별고우음성조(P=0.002화P=0.001).결론 본결과현시유선암중EMT여BLBC관계최밀절.EMT가능시조성BLBC여기타아형유선암생물학행위차이적심요원인.
Objectives To evaluate the relationship between epithelial- mesenchymal transition and basal cell-like phenotype breast cancer(BLBC). Methods Three hundred and eighty two cases of breast cancers including basal cell-like, luminal A, luminal B and Her-2 subtypes were collected from 458 cases of invasivc breast cancers based on their immunopbenotypos. They were then stained immunohistochemicallywith FOXC-2,vimentin,Syndecan-1 and E-cadherin. The relationship of these markers with the basal cell-like phenotype of breast cancer was studied. Results Of the 41 BLBC cases, FOXC-2, vimentin and Syndecan-1 were positive in 14 cases (34. 1% ), 18 cases (43.9%) and 36 cases (87.7%) respectively;E-cadherin expression was reduced in 26 cases (63.4%). The positive rates of FOXC-2 and vimentin were higher in BLBC than in other subtypes of breast cancer (P < 0. 01 ). The expression of E-cadherin was the lowest among the 4 subtypes of breast cancers ( P < 0. 01 ). Syndecan-1 was positive in the stromal cells adjacent to cancer cells in 17 cases (41.5%) BLBC and the expression was higher than that in other subtypes (P = 0. 007 ). There existed a correlation between FOXC-2 and vimenfin expression in BLBC (r =0. 607, P < 0. 01 ). The rates of positive lymph nodes in FOXC-2 and vimentin positive BLBC cases were 71.4% and 66. 7% respectively, and beth were higher than those of FOXC-2 and vimenfin negative BLBC cases ( P = 0. 002 and P = 0. 001 ). Conclusion Epithelial-mesenchymal transition is probably related to the basal cell-like phenotype of breast cancers, and this may be one of the reasons accounting for the different biological behavior of BLBC from other subtypes of breast cancer.