中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2008年
10期
780-784
,共5页
荣书玲%王庸晋%王晓林%王凤芝%杨刚%王裕勤%常超%曹恒%高焱章%卢永昕
榮書玲%王庸晉%王曉林%王鳳芝%楊剛%王裕勤%常超%曹恆%高焱章%盧永昕
영서령%왕용진%왕효림%왕봉지%양강%왕유근%상초%조항%고염장%로영흔
生长激素%心肌梗死%细胞因子类
生長激素%心肌梗死%細胞因子類
생장격소%심기경사%세포인자류
Growth hormone%Myocardial infarction%Cytokines
目的 观察重组人生长激素(rhGH)对大鼠急性心肌梗死后心功能及心肌炎性因子表达的影响. 方法 结扎大鼠左冠状动脉前降支建立心肌梗死模型,将术后24 h存活的大鼠随机分为对照组和rhGH组,另设假手术组.rhGH组给予rhGH 2 mg.kg-1.d-1皮下注射,对照组和假手术组给予等体积的生理盐水皮下注射,4周后观察rhGH对大鼠心功能及心肌细胞肿瘤坏死因子-α(TNF-α)、白介素-1p(IL-1p)、白介素-6(IL-6)、白介素-10(IL-10)mRNA表达的影响. 结果 与假手术组比较,对照组心肌组织中促炎性细胞因子TNF-α、IL-1β、IL-β和IL-10 mRNA表达(假手术组分别为0.10±0.02、0.08±0.01、0.18±0.01和0.14±0.05;梗死区分别为0.77±0.15、0.93±0.17、1.10±0.14和0.73±0.11;非梗死区分别为0.88±0.14、0.95±0.17、1.18±0.11和0.83±0.16)明显升高.与对照组比较,rhGH组心肌组织中TNF-α、IL-1β、IL-6 mRNA表达(梗死区分别为0.35±0.10、0.36±0.10、0.43±0.11;非梗死区分别为0.51±0.10、0.42±0.11、0.51±0.12)明显下降,IL-10 mRNA表达(梗死区为1.18±0.18;非梗死区为1.21±0.22)升高,P＜0.05.心脏超声显示rhGH组左室功能明显改善. 结论 早期rhGH治疗改善了心肌梗死大鼠心肌炎性因子表达和心脏功能.rhGH有效改善心功能与其降低心肌细胞促炎细胞因子和升高抗炎因子的作用有关.
目的 觀察重組人生長激素(rhGH)對大鼠急性心肌梗死後心功能及心肌炎性因子錶達的影響. 方法 結扎大鼠左冠狀動脈前降支建立心肌梗死模型,將術後24 h存活的大鼠隨機分為對照組和rhGH組,另設假手術組.rhGH組給予rhGH 2 mg.kg-1.d-1皮下註射,對照組和假手術組給予等體積的生理鹽水皮下註射,4週後觀察rhGH對大鼠心功能及心肌細胞腫瘤壞死因子-α(TNF-α)、白介素-1p(IL-1p)、白介素-6(IL-6)、白介素-10(IL-10)mRNA錶達的影響. 結果 與假手術組比較,對照組心肌組織中促炎性細胞因子TNF-α、IL-1β、IL-β和IL-10 mRNA錶達(假手術組分彆為0.10±0.02、0.08±0.01、0.18±0.01和0.14±0.05;梗死區分彆為0.77±0.15、0.93±0.17、1.10±0.14和0.73±0.11;非梗死區分彆為0.88±0.14、0.95±0.17、1.18±0.11和0.83±0.16)明顯升高.與對照組比較,rhGH組心肌組織中TNF-α、IL-1β、IL-6 mRNA錶達(梗死區分彆為0.35±0.10、0.36±0.10、0.43±0.11;非梗死區分彆為0.51±0.10、0.42±0.11、0.51±0.12)明顯下降,IL-10 mRNA錶達(梗死區為1.18±0.18;非梗死區為1.21±0.22)升高,P＜0.05.心髒超聲顯示rhGH組左室功能明顯改善. 結論 早期rhGH治療改善瞭心肌梗死大鼠心肌炎性因子錶達和心髒功能.rhGH有效改善心功能與其降低心肌細胞促炎細胞因子和升高抗炎因子的作用有關.
목적 관찰중조인생장격소(rhGH)대대서급성심기경사후심공능급심기염성인자표체적영향. 방법 결찰대서좌관상동맥전강지건립심기경사모형,장술후24 h존활적대서수궤분위대조조화rhGH조,령설가수술조.rhGH조급여rhGH 2 mg.kg-1.d-1피하주사,대조조화가수술조급여등체적적생리염수피하주사,4주후관찰rhGH대대서심공능급심기세포종류배사인자-α(TNF-α)、백개소-1p(IL-1p)、백개소-6(IL-6)、백개소-10(IL-10)mRNA표체적영향. 결과 여가수술조비교,대조조심기조직중촉염성세포인자TNF-α、IL-1β、IL-β화IL-10 mRNA표체(가수술조분별위0.10±0.02、0.08±0.01、0.18±0.01화0.14±0.05;경사구분별위0.77±0.15、0.93±0.17、1.10±0.14화0.73±0.11;비경사구분별위0.88±0.14、0.95±0.17、1.18±0.11화0.83±0.16)명현승고.여대조조비교,rhGH조심기조직중TNF-α、IL-1β、IL-6 mRNA표체(경사구분별위0.35±0.10、0.36±0.10、0.43±0.11;비경사구분별위0.51±0.10、0.42±0.11、0.51±0.12)명현하강,IL-10 mRNA표체(경사구위1.18±0.18;비경사구위1.21±0.22)승고,P＜0.05.심장초성현시rhGH조좌실공능명현개선. 결론 조기rhGH치료개선료심기경사대서심기염성인자표체화심장공능.rhGH유효개선심공능여기강저심기세포촉염세포인자화승고항염인자적작용유관.
Objective To explore the effects of recombinant human growth hormone(rhGH)on myocardial inflammatory cytokine expression and heart function in rats with acute myocardial infarction (AMI). Methods Rats with AMI induced by left anterior descending coronary branch ligation were randomized to rhGH and control groups compared with sham-operated group. The effects of 4 weeks of therapy with GH starting 24 hours after myocardial infarction on myocardial cytokines expression and heart function were studied. Myocardial inflammation was examined by analyzing the myocardial cytokine production including the pro-inflammatory cytokines: interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α and the anti-inflammatory cytokine: IL-10. Echocardiography was used to evaluate heart function. Results The levels of TNF-α, IL-1β, IL-6 and IL-10 in the infarcted and non-infarcted region of control group were markedly elevated compared to sham-operated group (all P＜0.05). After 4 weeks therapy, rhGH reduced the expression of TNF -α, IL-1β, IL-6 and increased IL-10 expression in the infarcted and non-infarcted region of rhGH group compared to control group (all P＜0. 05 ). Echocardiography showed that rhGH markedly improved left heart function (P＜0. 05 ). Conclusions Early rhGH treatment can improve heart function and myocardial inflammatory cytokine expression after AMI. One of immunopharmacologic mechanisms underlying the beneficial effects of rhGH on heart function improvement may involve the attenuation of pro-inflammatory cytokines and the increase of anti-inflammatory cytokine levels in cardiac myocytes.
