CAJ | 학술논문

目的探讨博尔纳病毒(BDV)在遵义市及周边地区流行状况.方法采用荧光定量巢式反转录酶聚合酶链反应(FQ-nRT-PCR)检测43例病毒性脑炎(VE)患者、9例多发性硬化(MS)患者、7例急性吉兰-巴雷综合征(GBS)患者、5例帕金森病(PD)患者、98名健康人外周血液单个核细胞(PBMC)、300只山羊PBMC中BDV p24基因片段.对阳性产物进行基因序列测定,氨基酸顺序及同源性分析,并分析BDV分子流行病学特点.结果 BDV p24基肉片段阳性率VE患者为13.95%,MS患者为22.22%,而GBS和PD患者及健康人中未检出.VE和MS患者检出率明显高于健康人(0%,P<0.05),山羊BDV p24基冈片段阳性率为0.67%,与健康人对比差异尢统计学意义(P>0.05);VE和MS患者测序结果相同与GenBank提供的strain V毒株比较同源性为96.51%,有3个位点出现一致性沉寂突变,BDV/MDCK毒株比较同源性为97.67%,有2个位点出现一致性沉寂突变,与C6BV毒株比较有2个位点出现一致性沉寂突变,其山羊中BDV p24基因片段测序结果与GenBank提供的strain V毒株比较同源性为96.51%,有3个位点出现一致性沉寂突变,与BDV/MDCK毒株比较同源性为96.51%,有3个位点出现一致性沉寂突变,与C6BV毒株比较同源性为96.51%,有3个位点出现一致性沉寂突变,但所编码的氨基酸没有改变.结论遵义市及周边部分地区VE、MS可能与BDV感染有关,人类感染BDV可能存在动物源性.
목적 탐토박이납병독(BDV)재준의시급주변지구류행상황.방법 채용형광정량소식반전록매취합매련반응(FQ-nRT-PCR)검측43례병독성뇌염(VE)환자、9례다발성경화(MS)환자、7례급성길란-파뢰종합정(GBS)환자、5례파금삼병(PD)환자、98명건강인외주혈액단개핵세포(PBMC)、300지산양PBMC중BDV p24기인편단.대양성산물진행기인서렬측정,안기산순서급동원성분석,병분석BDV분자류행병학특점.결과 BDV p24기육편단양성솔VE환자위13.95%,MS환자위22.22%,이GBS화PD환자급건강인중미검출.VE화MS환자검출솔명현고우건강인(0%,P<0.05),산양BDV p24기강편단양성솔위0.67%,여건강인대비차이왕통계학의의(P>0.05);VE화MS환자측서결과 상동여GenBank제공적strain V독주비교동원성위96.51%,유3개위점출현일치성침적돌변,BDV/MDCK독주비교동원성위97.67%,유2개위점출현일치성침적돌변,여C6BV독주비교유2개위점출현일치성침적돌변,기산양중BDV p24기인편단측서결과 여GenBank제공적strain V독주비교동원성위96.51%,유3개위점출현일치성침적돌변,여BDV/MDCK독주비교동원성위96.51%,유3개위점출현일치성침적돌변,여C6BV독주비교동원성위96.51%,유3개위점출현일치성침적돌변,단소편마적안기산몰유개변.결론 준의시급주변부분지구VE、MS가능여BDV감염유관,인류감염BDV가능존재동물원성.
Objective In order to study the epidemiology of Borna disease virus (BDV) in Zunyi region and its surrounding regions in Guizhou province. Methods p24 fragment of BDV fragments in peripheral blood mononuclear cells (PBMC) from 43 patients with viral encephalitis (VE), 9 cases with multiple sclerosis (MS), 7 cases with Guillain-Barre syndrome (GBS), 5 cases with Parkinson disease (PD), 98 healthy donors and 300 goats were examined by quantitative fluorescence nested reverse transcriptase polymerase chain reaction (PCR). Gene sequence and amino acid sequence were analyzed for positive products. Results The positive rate of BDV p24 fragment in PBMC from VE (13.95 %) and MS (22.22 %) were significantly higher than in healthy donors (0 %, P<0.05). The positive rate of BDV p24 fragment in PBMC from goats was 0.67 %, without statistical difference when compared with healthy donors (P>0.05). Guillain-Barre syndrome and Parkinson disease( PD)were tested negative. The sequence of the BDV p24 fragment from the patients with VE was in conformity with that of the MS. Results presented that 3 situs consistency silent mutation when compared with strain V and its homogeneity was 96.51%. 2 situs consistency silent mutation compared with BDV/MDCK and its homogeneity was 97.67%. 2 situs consistency silent mutation when compared with C6BV and its homogeneity was 97.67 %. Sequences of the BDV p24 fragment from the goats presented 3 situs consistency silent mutation when compared with strain V and its homogeneity was 96.51%. 3 situs consistency silent mutation when compared with BDV/MDCK and its homogeneity was 96.51%. 3 situs consistency silent mutation when compared with C6BV and its homogeneity was 96.51%. However, there were no changes of encoding amino acids in all BDV p24 fragments from neuropsychiatric disorders. Conclusion Our data indicated that the BDV infection was presented in patients with VE, MS and goats from Zunyi region and its surrounding regions of Guizhou province. BDV might play a potential role in the development of VE, MS as well as having correlations with animals.