中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2011年
5期
481-484
,共4页
王鹏%赵飞%郭英%苏鹏%韦蝶心%张建中%董兴齐
王鵬%趙飛%郭英%囌鵬%韋蝶心%張建中%董興齊
왕붕%조비%곽영%소붕%위접심%장건중%동흥제
鼠疫菌苗%pYC质粒%蛋白质组%电泳,凝胶,双向
鼠疫菌苗%pYC質粒%蛋白質組%電泳,凝膠,雙嚮
서역균묘%pYC질립%단백질조%전영,응효,쌍향
Yersinia pestis%Plasmid pYC%Proteome%Electrophoresis,gel,two-dimensional
目的 了解鼠疫菌pYC质粒对菌体蛋白质组的作用。方法 将含有pYC质粒的鼠疫菌与不含该质粒的鼠疫菌通过双向电泳的方法进行蛋白质组比对，并将差异蛋白进行质谱鉴定。结果 含有pYC质粒的鼠疫菌与不含该质粒的鼠疫菌的蛋白图谱可识别的蛋白点均在500个以上，两者总体上相似性较高;前者较后者在大小约70×103、等电点5.0左右处，明显多一个蛋白点簇，该蛋白簇经质谱鉴定均为伴侣蛋白GroEL，但该蛋白并非是pYC质粒编码蛋白。结论 pYC质粒对菌体蛋白质组有影响，其编码蛋白pYC_p10与pYC_p11，可能调控GroEL高表达。
目的 瞭解鼠疫菌pYC質粒對菌體蛋白質組的作用。方法 將含有pYC質粒的鼠疫菌與不含該質粒的鼠疫菌通過雙嚮電泳的方法進行蛋白質組比對，併將差異蛋白進行質譜鑒定。結果 含有pYC質粒的鼠疫菌與不含該質粒的鼠疫菌的蛋白圖譜可識彆的蛋白點均在500箇以上，兩者總體上相似性較高;前者較後者在大小約70×103、等電點5.0左右處，明顯多一箇蛋白點簇，該蛋白簇經質譜鑒定均為伴侶蛋白GroEL，但該蛋白併非是pYC質粒編碼蛋白。結論 pYC質粒對菌體蛋白質組有影響，其編碼蛋白pYC_p10與pYC_p11，可能調控GroEL高錶達。
목적 료해서역균pYC질립대균체단백질조적작용。방법 장함유pYC질립적서역균여불함해질립적서역균통과쌍향전영적방법진행단백질조비대，병장차이단백진행질보감정。결과 함유pYC질립적서역균여불함해질립적서역균적단백도보가식별적단백점균재500개이상，량자총체상상사성교고;전자교후자재대소약70×103、등전점5.0좌우처，명현다일개단백점족，해단백족경질보감정균위반려단백GroEL，단해단백병비시pYC질립편마단백。결론 pYC질립대균체단백질조유영향，기편마단백pYC_p10여pYC_p11，가능조공GroEL고표체。
Objective To investigate the role of plasmid pYC on proteome of Yersinia pestis. Methods Two dimensional electrophoresis was performed to strains of Yersinia pestis with and without the pYC plasmid, and differential proteins were identified by mass spectrometry. Results More than 500 protein spots of Yersinia pestis with and without plasmid pYC were recognized, and their protein profiles were generally similar. The chaperone GroEL was highly expressed in strains with plasmid pYC, whereas the protein GroEL was not encoded by plasmid pYC. Conclusions Plasmid pYC has an impact on proteome of Yersiniapestis. The proteins of pYC-p10 and pYC-p11 encoded by plasmid pYC may regulate the expression of GroEL.
