中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
4期
447-450
,共4页
刘春安%张新东%杨艳%马凯%白景芝%王祥阁%王静%柴同海
劉春安%張新東%楊豔%馬凱%白景芝%王祥閣%王靜%柴同海
류춘안%장신동%양염%마개%백경지%왕상각%왕정%시동해
碘化合物/诊断应用%染色与标记%基因%p53%微管相关蛋白质类%端粒%末端转移酶%食管肿瘤/诊断
碘化閤物/診斷應用%染色與標記%基因%p53%微管相關蛋白質類%耑粒%末耑轉移酶%食管腫瘤/診斷
전화합물/진단응용%염색여표기%기인%p53%미관상관단백질류%단립%말단전이매%식관종류/진단
Iodine componnds/DU%Staining and labeling%Genes,p53%Microtubule-associated proteins%Telomerase%Esophageal neoplasms/DI
目的 探讨内镜下碘染色联合组织p53、Survivin及端粒酶检测对早期食管癌及癌前病变的诊断价值.方法 本院因上消化道症状接受胃镜检查且食管黏膜有可疑病变的门诊及住院患者300例进行卢戈碘液染色.对直径≥5 mm的不染区或淡柒区取活检送病理.用免疫组化方法 对病理组织学检查证实为黏膜不典型增生、早期食管癌及30例正常对照者分别检测p53、Sur-vivin及端粒酶表达情况.结果 碘染色对食管黏膜不典型增生轻、中、重度及早期鳞状细胞癌检出率分别为16.3%、9.7%、3.7%和4.3%.不典型增生及早癌组织中p53蛋白、Survivin及端粒酶表达相关性有统计学意义,并且碘不染色与p53、Survivin及端粒酶阳性表达相关性也有统计学意义(P均<0.01);碘染色联合p53、Survlvin及端粒酶检测对重度不典型增生和早期食管癌诊断的敏感性、特异性和准确率分别达100%、92.3%和91.8%.结论 碘染色联合p53、Survivin及端粒酶检测对诊断早期食管癌及其癌前病变具有重要价值.
目的 探討內鏡下碘染色聯閤組織p53、Survivin及耑粒酶檢測對早期食管癌及癌前病變的診斷價值.方法 本院因上消化道癥狀接受胃鏡檢查且食管黏膜有可疑病變的門診及住院患者300例進行盧戈碘液染色.對直徑≥5 mm的不染區或淡柒區取活檢送病理.用免疫組化方法 對病理組織學檢查證實為黏膜不典型增生、早期食管癌及30例正常對照者分彆檢測p53、Sur-vivin及耑粒酶錶達情況.結果 碘染色對食管黏膜不典型增生輕、中、重度及早期鱗狀細胞癌檢齣率分彆為16.3%、9.7%、3.7%和4.3%.不典型增生及早癌組織中p53蛋白、Survivin及耑粒酶錶達相關性有統計學意義,併且碘不染色與p53、Survivin及耑粒酶暘性錶達相關性也有統計學意義(P均<0.01);碘染色聯閤p53、Survlvin及耑粒酶檢測對重度不典型增生和早期食管癌診斷的敏感性、特異性和準確率分彆達100%、92.3%和91.8%.結論 碘染色聯閤p53、Survivin及耑粒酶檢測對診斷早期食管癌及其癌前病變具有重要價值.
목적 탐토내경하전염색연합조직p53、Survivin급단립매검측대조기식관암급암전병변적진단개치.방법 본원인상소화도증상접수위경검사차식관점막유가의병변적문진급주원환자300례진행로과전액염색.대직경≥5 mm적불염구혹담칠구취활검송병리.용면역조화방법 대병리조직학검사증실위점막불전형증생、조기식관암급30례정상대조자분별검측p53、Sur-vivin급단립매표체정황.결과 전염색대식관점막불전형증생경、중、중도급조기린상세포암검출솔분별위16.3%、9.7%、3.7%화4.3%.불전형증생급조암조직중p53단백、Survivin급단립매표체상관성유통계학의의,병차전불염색여p53、Survivin급단립매양성표체상관성야유통계학의의(P균<0.01);전염색연합p53、Survlvin급단립매검측대중도불전형증생화조기식관암진단적민감성、특이성화준학솔분별체100%、92.3%화91.8%.결론 전염색연합p53、Survivin급단립매검측대진단조기식관암급기암전병변구유중요개치.
Objective To study the value of using endoscopy iodine staining combined detecting the expression of p53, survivin and telomerase in the diagnosis of early esophageal carcinoma and precancerous lesions. Methods Three hundred patients who received routine gastrescopy and had high risk of esophageal carcinoma were examined by the iodine dyeing on their esophageal mucosa with 2. 5% Lugol solu-tion. Multiple biopsies were taken from the unstained or light-stained areas when their diameters were over 5mm for pathology diagnosis. The expression of p53, survivin and telomerase were detected by immunohistochemical staining. Results With the Lugol staining method, the incidences of light, moderate and high grade dysplasia and early esophageal carcinoma were 16. 3% ,9. 7% ,3.7% and 4. 3% respectively. The expression of p53, survivin and telomerase were related and the unstained were related to the expression of p53, survivin and telomerase in dysplasia and early esophageal carcinoma. Using endoscopy iodine staining combined testing p53, survivin and telomerase showed sensitiv-ity of 100%, specificity of 92. 3% and accuracy of 91.8% to diagnose of early esophageal carcinoma and high grade dysplasia. Conclusion Lugol solution chromoendscopy combined assay of p53, survivin and telomersse might be useful in the diagnosing early esophageal carcino-ma and precancerous lesion.
