CAJ | 학술논문

目的观察胶质瘤裸鼠移植瘤生长过程中的血管生成拟态现象(VM)以及与血管内皮依赖性血管之间的关系.方法采用人脑胶质瘤细胞株U87单细胞悬液(2×106个/0.1 ml)于4周龄裸鼠皮下注射建立移植瘤模型.应用血管内皮细胞标志物CD34和高碘酸雪夫(PAS)试剂双染的方法检测40例不同大小的皮下移植瘤中的VM和血管内皮依赖性血管.结果 VM和血管内皮依赖性血管在不同大小的裸鼠皮下U87移植瘤中均存在.微血管密度(MVD)和肿瘤的直径呈正相关(r=0.393,P=0.012),血管生成拟态密度(VMD)与MVD负相关(r=-0.404,P=0.010),而与肿瘤的直径没有显著的相关性(P =0.575).结论血管生成拟态与内皮依赖性血管是两种不同的肿瘤循环形式,在U87裸鼠皮下移植瘤的生长过程中持续存在并具有互补性,共同为肿瘤的生长提供营养支持.
목적 관찰효질류라서이식류생장과정중적혈관생성의태현상(VM)이급여혈관내피의뢰성혈관지간적관계.방법 채용인뇌효질류세포주U87단세포현액(2×106개/0.1 ml)우4주령라서피하주사건립이식류모형.응용혈관내피세포표지물CD34화고전산설부(PAS)시제쌍염적방법검측40례불동대소적피하이식류중적VM화혈관내피의뢰성혈관.결과 VM화혈관내피의뢰성혈관재불동대소적라서피하U87이식류중균존재.미혈관밀도(MVD)화종류적직경정정상관(r=0.393,P=0.012),혈관생성의태밀도(VMD)여MVD부상관(r=-0.404,P=0.010),이여종류적직경몰유현저적상관성(P =0.575).결론 혈관생성의태여내피의뢰성혈관시량충불동적종류순배형식,재U87라서피하이식류적생장과정중지속존재병구유호보성,공동위종류적생장제공영양지지.
Objective This study was designed to observe the vasculogenic mimicry (VM)and the relationship between VM and endothelial - dependent vessels during the growth of subcutaneous xenograft glioma in Balb/c mice.Methods Forty 4 - week - old male Balb/c mice were inoculated with a single cell suspension of U87 (2 × 106 tumor cells were iajected subcutaneously each mouse).The VM and endothelial - dependent vessels were detected by dual staining for endothelial marker CD34 and periodic acid - Schiff (PAS).The density of endothelium - dependent microvessels and VM was measured in tumor tissue sections by 5 randomly selected fields at 200 magnification and the average counts were then calculated.Results Endothelial - dependent vessels as well as VM in xenografts of U87 were persistent exist during the process of tumor growth.With the increase of tumor size,microvessel density (MVD) increased ( r=0.393,P =0.012),while vasculogenic mimicry density (VMD) was no significant change (P =0.575).The VMD were negative correlated with MVD ( r =-0.404,P =0.010).Conclusions There are two microcirculation patterns exist in xenografts of U87 in Balb/c mice during tumor growth,including VM and endothelial - dependent vessels.Both VM and endothelial - dependent vessels provide nutrition and oxygen for tumor development.