中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2012年
9期
729-732
,共4页
朱琳%潘学谊%关则兵%郭煜%李铭杰%曾文彬%黄方
硃琳%潘學誼%關則兵%郭煜%李銘傑%曾文彬%黃方
주림%반학의%관칙병%곽욱%리명걸%증문빈%황방
脑梗死%脑信号蛋白%sSema4D
腦梗死%腦信號蛋白%sSema4D
뇌경사%뇌신호단백%sSema4D
Cerebral infarction%Semaphorins%sSema 4D
目的 探讨脑梗死患者外周血淋巴细胞脑信号蛋白4D(Semaphorin4D,Sema4D)mRNA、血小板表面Sema4D的表达和血浆可溶性Sema4D(sSema4D)水平及其临床意义.方法 选取脑梗死急性期患者299例,以195名健康体检者为对照组.用Real-time PCR法测定外周血淋巴细胞Sema4DmRNA表达,用流式细胞术检测血小板表面Sema4D表达,用ELISA法测定血浆sSemMD浓度.其中195例急性期患者常规治疗2周后,测定血小板表面Sema4D表达及血浆sSema4D浓度.结果 脑梗死急性期患者Sema4D mRNA拷贝数[(2.23,2.66)× 104IU/m]]及血浆sSema4D浓度[(1.34±0.56)μg/L]明显高于正常对照组[(0.49,0.53)×103Iu/ml、(0.61±0.31)μg/L],血小板表面Sema4D表达(191.62±46.56)较正常对照组(303.33±112.66)明显下调,差异有统计学意义(P<0.01).脑梗死急性期患者血小板表面Sema4D表达与血浆sSema4D水平呈负相关(r=-0.328,P<0.01).治疗2周后脑梗死患者的血小板表面Sema4D水平较急性期回升,血浆sSema4D浓度较急性期下降.均接近正常水平.结论 脑梗死急性期患者淋巴细胞Sema4D mRNA表达及血浆sSema4D水平升高、血小板表面Sema4D表达降低;治疗2周后血小板Sema4D表达及血浆sSema4D水平恢复正常.Sema4D可能是一种反映脑梗死发生的急性时相蛋白.
目的 探討腦梗死患者外週血淋巴細胞腦信號蛋白4D(Semaphorin4D,Sema4D)mRNA、血小闆錶麵Sema4D的錶達和血漿可溶性Sema4D(sSema4D)水平及其臨床意義.方法 選取腦梗死急性期患者299例,以195名健康體檢者為對照組.用Real-time PCR法測定外週血淋巴細胞Sema4DmRNA錶達,用流式細胞術檢測血小闆錶麵Sema4D錶達,用ELISA法測定血漿sSemMD濃度.其中195例急性期患者常規治療2週後,測定血小闆錶麵Sema4D錶達及血漿sSema4D濃度.結果 腦梗死急性期患者Sema4D mRNA拷貝數[(2.23,2.66)× 104IU/m]]及血漿sSema4D濃度[(1.34±0.56)μg/L]明顯高于正常對照組[(0.49,0.53)×103Iu/ml、(0.61±0.31)μg/L],血小闆錶麵Sema4D錶達(191.62±46.56)較正常對照組(303.33±112.66)明顯下調,差異有統計學意義(P<0.01).腦梗死急性期患者血小闆錶麵Sema4D錶達與血漿sSema4D水平呈負相關(r=-0.328,P<0.01).治療2週後腦梗死患者的血小闆錶麵Sema4D水平較急性期迴升,血漿sSema4D濃度較急性期下降.均接近正常水平.結論 腦梗死急性期患者淋巴細胞Sema4D mRNA錶達及血漿sSema4D水平升高、血小闆錶麵Sema4D錶達降低;治療2週後血小闆Sema4D錶達及血漿sSema4D水平恢複正常.Sema4D可能是一種反映腦梗死髮生的急性時相蛋白.
목적 탐토뇌경사환자외주혈림파세포뇌신호단백4D(Semaphorin4D,Sema4D)mRNA、혈소판표면Sema4D적표체화혈장가용성Sema4D(sSema4D)수평급기림상의의.방법 선취뇌경사급성기환자299례,이195명건강체검자위대조조.용Real-time PCR법측정외주혈림파세포Sema4DmRNA표체,용류식세포술검측혈소판표면Sema4D표체,용ELISA법측정혈장sSemMD농도.기중195례급성기환자상규치료2주후,측정혈소판표면Sema4D표체급혈장sSema4D농도.결과 뇌경사급성기환자Sema4D mRNA고패수[(2.23,2.66)× 104IU/m]]급혈장sSema4D농도[(1.34±0.56)μg/L]명현고우정상대조조[(0.49,0.53)×103Iu/ml、(0.61±0.31)μg/L],혈소판표면Sema4D표체(191.62±46.56)교정상대조조(303.33±112.66)명현하조,차이유통계학의의(P<0.01).뇌경사급성기환자혈소판표면Sema4D표체여혈장sSema4D수평정부상관(r=-0.328,P<0.01).치료2주후뇌경사환자적혈소판표면Sema4D수평교급성기회승,혈장sSema4D농도교급성기하강.균접근정상수평.결론 뇌경사급성기환자림파세포Sema4D mRNA표체급혈장sSema4D수평승고、혈소판표면Sema4D표체강저;치료2주후혈소판Sema4D표체급혈장sSema4D수평회복정상.Sema4D가능시일충반영뇌경사발생적급성시상단백.
Objective To explore the expression and clinical significance of Semaphorin4D (Sema4D)mRNA in peripheral blood lymphocyte.Sema4D on platelet surface,soluble SemMD(sSema4D) in plasma in patients with cerebral infarction.Methotis Taking 299 patients with cerebral infarction as the case group while l 95 healthy adults as the control group.The mRNA expression of Sema4D was detected by Real-time PCR,and SemacD expression on platelet by flow cytometry,sSema4D by ELISA.Then,the expression of Sema4D on platelet surface and the concentration of sSema4D iIi plasma of the 195 selected patients following 2 weeks'treatment were tested.Results The expression of Sema4D mRNA significantly increased in the case group[(2.23,2.66)×107IU/ml]than in the control group[(0.49,0.53)× 104IU/ml](P<0.01).The level of Sema4D on platelet surface in the case group(191.62±46.56)significantly decreased than in the control group(303.33 ±112.66)(P<0.01).But the concentration of sSema4D in plasma in the case group[(1.34±0.56)μg/L] was obviously higher than in the control group[(0.61±0.31)μg/L](P<0.01).The expression of Sema4D Oil platelet was obviously relevant with the concentration of sSema4D in plasma in the case group with the correlation coefficient as 0.328(P<0.01).The expression of Sema4D on platelet obviously peaked up following 2 weeks'routine therapy in the case group,which was close to that in the control group.Meanwhile the concentration of sSema4D in plasma Was downward corrected to the normal in the case group.Conclusion The iflcreased expressions and plasma levels.and redueed expressions on platelet of Sema4D in acute period.which returned to norlnal 2 weeks after treatment in the case group may be related to the ocenrrence of acute cerebral infarction,reflecting the development process of cerebral infarction.
