中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2010年
2期
1-3
,共3页
肝细胞癌%HCCLM3%凝血酶敏感蛋白-1%凋亡%Caspase-3
肝細胞癌%HCCLM3%凝血酶敏感蛋白-1%凋亡%Caspase-3
간세포암%HCCLM3%응혈매민감단백-1%조망%Caspase-3
Hepatocellur carcinoma%Thrombospondin-1%HCCLM3%Apoptosis%Caspase-3
目的 研究凝血酶敏感蛋白-1(TSP-1)对人肝癌细胞株HCCLM3凋亡的体外诱导作用及机制.方法 采用流式细胞术检测TSP-1及其受体CD36、CD47诱导HCCLM3的细胞凋亡率,应用电镜分析作用后的形态变化,逆转录聚合酶链反应(RT-PCR)分析作用后HCCLM3细胞Caspase-3 mRNA表达的变化.结果 TSP-1组凋亡率[(12.44±0.72)%]显著高于对照组[(4.31±0.29)%]和CD47阻断组[(4.99±0.12)%],P<0.01.CD36阻断组[(9.99±0.57)%]高于对照组或CD47阻断组,低于TSP-1组(P<0.01).电镜观察:对照组和C1D47阻断组细胞生长旺盛.TSP-1组和CD36阻断组细胞凋亡率增加,细胞呈现各种凋亡的表现.TSP-1组Caspase-3 mRNA的表达TSP-1组(0.652±0.024)和CD36阻断组(0.615±0.020)显著高于对照组(0.398±0.033)和CD47阻断组(0.432±0.019),P<0.01.结论 TSP-1可诱导人肝癌细胞株HCCLM3的凋亡,TSP-1与受体CD47结合后上调Caspase-3的表达可能是作用途径之一.
目的 研究凝血酶敏感蛋白-1(TSP-1)對人肝癌細胞株HCCLM3凋亡的體外誘導作用及機製.方法 採用流式細胞術檢測TSP-1及其受體CD36、CD47誘導HCCLM3的細胞凋亡率,應用電鏡分析作用後的形態變化,逆轉錄聚閤酶鏈反應(RT-PCR)分析作用後HCCLM3細胞Caspase-3 mRNA錶達的變化.結果 TSP-1組凋亡率[(12.44±0.72)%]顯著高于對照組[(4.31±0.29)%]和CD47阻斷組[(4.99±0.12)%],P<0.01.CD36阻斷組[(9.99±0.57)%]高于對照組或CD47阻斷組,低于TSP-1組(P<0.01).電鏡觀察:對照組和C1D47阻斷組細胞生長旺盛.TSP-1組和CD36阻斷組細胞凋亡率增加,細胞呈現各種凋亡的錶現.TSP-1組Caspase-3 mRNA的錶達TSP-1組(0.652±0.024)和CD36阻斷組(0.615±0.020)顯著高于對照組(0.398±0.033)和CD47阻斷組(0.432±0.019),P<0.01.結論 TSP-1可誘導人肝癌細胞株HCCLM3的凋亡,TSP-1與受體CD47結閤後上調Caspase-3的錶達可能是作用途徑之一.
목적 연구응혈매민감단백-1(TSP-1)대인간암세포주HCCLM3조망적체외유도작용급궤제.방법 채용류식세포술검측TSP-1급기수체CD36、CD47유도HCCLM3적세포조망솔,응용전경분석작용후적형태변화,역전록취합매련반응(RT-PCR)분석작용후HCCLM3세포Caspase-3 mRNA표체적변화.결과 TSP-1조조망솔[(12.44±0.72)%]현저고우대조조[(4.31±0.29)%]화CD47조단조[(4.99±0.12)%],P<0.01.CD36조단조[(9.99±0.57)%]고우대조조혹CD47조단조,저우TSP-1조(P<0.01).전경관찰:대조조화C1D47조단조세포생장왕성.TSP-1조화CD36조단조세포조망솔증가,세포정현각충조망적표현.TSP-1조Caspase-3 mRNA적표체TSP-1조(0.652±0.024)화CD36조단조(0.615±0.020)현저고우대조조(0.398±0.033)화CD47조단조(0.432±0.019),P<0.01.결론 TSP-1가유도인간암세포주HCCLM3적조망,TSP-1여수체CD47결합후상조Caspase-3적표체가능시작용도경지일.
Objective To study the effect and mechanism of thrombospondin- 1 on the apoptosis of human hepatocarcinoma cell HCCLM3. Methods Detecting the role of TSP-1, CD36, CD47 on apoptosis rate of HCCLM3 by the flow cytometry and the change of cell ultractructure by transmission electron microscope,then detecting the influence of TSP-1, CD36, CD47 on the expression of Caspase-3 in HCCLM3. Results The apoptosis rate was respectively significantly higher in TSP-1 group [(12. 44 ±0. 72)%] than that in control group [(4. 31 ±0. 29)%] or CD47-blocked group[(4. 99 ±0. 12)%],P < 0. 01. The apoptosis rate was was respectively significantly higher in CD36 -blocked group [(9. 99 ±0. 57)%]than that in control group and CD47 -blocked group,was lower than in the TSP-1 group(P <0. 01). The morphological changes were: grow vigorously and many divided nuleus in control group and CD47- blocked group. Apoptosis cell increased in TSP- 1 group and CD36 -blocked group: less cellular module in cytoplasm, many nucleus pieces and concentrate. The expression of Caspase-3 were higher in TSP-1 group (0. 652 ± 0. 024) and CD36-blocked group (0. 615 ± 0. 020) than that in control group (0. 398 ±0. 033)and CD47-blocked group (0. 432 ± 0. 019) (P < 0. 01). Conclusions TSP-1 can induce apoptosis of HCCLM3 cell line through combined with CD47 which can increase the expression Caspase -3.
