CAJ | 학술논문

目的通过分析急性肝损伤动物模型的31 P磁共振波谱学(MRS)能量代谢变化与肝损伤指数(LDS)之间的相关性,探讨31 P MRS对急性肝损伤的检测价值.方法将30只家兔随机分别一次性接受5～20 Gy不同剂量辐射,在辐射后24 h进行血液及31 P MRS检测.按照LDS不同积分将其分为轻(LDS≤3 U)、中(3 U＜LDS≤6 U)、重(LDS＞6 U)三组.另取10只健康家兔作为正常对照组.31P MRS检测采用1.5 T磁共振扫描仪的2维化学移动成像技术,检测内容包括磷酸单脂(PME)、无机磷(Pi)、磷酸二脂(PDE)、三磷酸腺苷(ATP)的相对量及其比值.结果 ①ATP相对量在正常对照组、轻度肝损伤组、中度肝损伤组和重度肝损伤组分别为1.83±0.33、1.55±0.24、1.27±0.07和0.98±0.18.各组间差异有统计学意义(P＜0.05),且随着肝损伤程度的加重,ATP相对量逐渐下降;重度肝损伤组的PME、Pi相对量减少,与正常对照组比较差异均有统计学意义(P＜0.05);PDE相对量各组之间差异均无统计学意义(P＞0.05).②仅中、重度肝损伤组的PDE/ATP比值升高,分别为1.94±0.50和1.96±0.72,与正常对照组(1.43±0.31)和轻度肝损伤组(1.40±0.38)间差异有统计学意义(P＜0.05);其他各化合物组间比值差异均无统计学意义(P＞0.05).结论 31P MRS是评价急性肝损伤的有效方法,ATP相对量水平与LDS之间具有明显相关性,它可准确反映家兔急性辐射性肝损伤及其严重程度.
목적 통과분석급성간손상동물모형적31 P자공진파보학(MRS)능량대사변화여간손상지수(LDS)지간적상관성,탐토31 P MRS대급성간손상적검측개치.방법 장30지가토수궤분별일차성접수5～20 Gy불동제량복사,재복사후24 h진행혈액급31 P MRS검측.안조LDS불동적분장기분위경(LDS≤3 U)、중(3 U＜LDS≤6 U)、중(LDS＞6 U)삼조.령취10지건강가토작위정상대조조.31P MRS검측채용1.5 T자공진소묘의적2유화학이동성상기술,검측내용포괄린산단지(PME)、무궤린(Pi)、린산이지(PDE)、삼린산선감(ATP)적상대량급기비치.결과 ①ATP상대량재정상대조조、경도간손상조、중도간손상조화중도간손상조분별위1.83±0.33、1.55±0.24、1.27±0.07화0.98±0.18.각조간차이유통계학의의(P＜0.05),차수착간손상정도적가중,ATP상대량축점하강;중도간손상조적PME、Pi상대량감소,여정상대조조비교차이균유통계학의의(P＜0.05);PDE상대량각조지간차이균무통계학의의(P＞0.05).②부중、중도간손상조적PDE/ATP비치승고,분별위1.94±0.50화1.96±0.72,여정상대조조(1.43±0.31)화경도간손상조(1.40±0.38)간차이유통계학의의(P＜0.05);기타각화합물조간비치차이균무통계학의의(P＞0.05).결론 31P MRS시평개급성간손상적유효방법,ATP상대량수평여LDS지간구유명현상관성,타가준학반영가토급성복사성간손상급기엄중정도.
Objective To study the relationship between energy metabolic changes tested by phosphorus-31 magnetic resonance(MR)spectroscopy(31P MRS)and the liver damage score(LDS)in rabbit models and investigate the diagnostic value of 31P MRS in acute hepatic injury.Methods A total of 30 rabbits were received different radiation dose(ranging from 5 Gy to 20 Gy)to establish acute hepatic injury models.31P MRS was than carried out 24 hrs after radiation.The rabbits were divided into mild(LDS≤3 U),moderate(LDS 3-6 U)and severe(LDS＞6 U)hepatie injury groups.Ten healthy rabbits were served as controls.MR examination was performed on a 1.5 T imager using a 1H/31P surface coil by the 2D chemical shift imaging technique.The relative quantification of phosphomonoesters(PME),phosphodiesters (PDE), inorganic phosphate (Pi) and adenosine severe hepatic injury groups were 1.83±0.33,1.55±0.24,1.27±0.07 and 0.98±0.18,respectively,with significant differences(P＜0.05).The ATP level was progressively decreased with the increase of severity Of hepatic injury.The relative quantification of PME and Pi were decreased in severe hepatic injury group compared to control group(P＜0.05).There was no significant difference higher in moderate(1.94±0.50)and severe(1.96±0.72)hepatic injury groups compared to control group(1.43±0.31)and mild hepatic injury group(1.40+0.38)(P＜0.05).No significant difference was found in ratio of relative quantification of other phosphorus metabolites.Conclusions 31P MRS is a useful method in evaluating acute hepatic injury.The relative quantification of hepatic ATP level,which can reflect the severity of acute hepatic injury,is correlated with LDS.