动物学报
動物學報
동물학보
ACTA ZOOLOGICA SINICA
2004年
5期
770-777
,共8页
白斑迷蛱蝶 视觉系统 γ-氨基丁酸(GABA) 5羟色胺(5-HT) 免疫组织化学
白斑迷蛺蝶 視覺繫統 γ-氨基丁痠(GABA) 5羥色胺(5-HT) 免疫組織化學
백반미협접 시각계통 γ-안기정산(GABA) 5간색알(5-HT) 면역조직화학
Minathyma schrenckii%Visual system%γ-aminobutyric acid (GABA)%Serotonin (5-HT)%Immunohisto-chemistry
采用树脂石蜡(Colophony-Paraffin,CP)组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-peroxidase,SP)免疫组织化学方法,首次报道了GABA和5-HT两种神经递质在白斑迷蛱蝶视觉系统(复眼及视叶)中的分布.与以往所报道的昆虫不同,白斑迷蛱蝶复眼中部分光感细胞对GABA和5-HT抗血清产生免疫反应.每侧视叶中约有2 600多个GABA能阳性神经元,它们共分为6群.其中3群位于外髓附近(M1-3),另外三群位于内髓复合体边缘(LC1-3).GABA能神经元发出的轴突在整个视叶的3个神经纤维网中都有分布.相比之下,视叶对5-HT抗血清的反应较弱,视叶神经纤维网中不存在代表5-HT阳性反应的粗大静脉曲张状纤维,只有一些排列规则的细小纤维.每侧视叶只有位于外髓附近的25个神经元呈现阳性反应,它们的分布位置与部分M3群的GABA能样神经元相同.本文还探讨了5-HT和GABA在调节视觉信息时可能发挥的作用[动物学报50(5):770-777,2004].
採用樹脂石蠟(Colophony-Paraffin,CP)組織包埋切片技術和鏈黴菌抗生物素蛋白-過氧化物酶(Streptavidin-peroxidase,SP)免疫組織化學方法,首次報道瞭GABA和5-HT兩種神經遞質在白斑迷蛺蝶視覺繫統(複眼及視葉)中的分佈.與以往所報道的昆蟲不同,白斑迷蛺蝶複眼中部分光感細胞對GABA和5-HT抗血清產生免疫反應.每側視葉中約有2 600多箇GABA能暘性神經元,它們共分為6群.其中3群位于外髓附近(M1-3),另外三群位于內髓複閤體邊緣(LC1-3).GABA能神經元髮齣的軸突在整箇視葉的3箇神經纖維網中都有分佈.相比之下,視葉對5-HT抗血清的反應較弱,視葉神經纖維網中不存在代錶5-HT暘性反應的粗大靜脈麯張狀纖維,隻有一些排列規則的細小纖維.每側視葉隻有位于外髓附近的25箇神經元呈現暘性反應,它們的分佈位置與部分M3群的GABA能樣神經元相同.本文還探討瞭5-HT和GABA在調節視覺信息時可能髮揮的作用[動物學報50(5):770-777,2004].
채용수지석사(Colophony-Paraffin,CP)조직포매절편기술화련매균항생물소단백-과양화물매(Streptavidin-peroxidase,SP)면역조직화학방법,수차보도료GABA화5-HT량충신경체질재백반미협접시각계통(복안급시협)중적분포.여이왕소보도적곤충불동,백반미협접복안중부분광감세포대GABA화5-HT항혈청산생면역반응.매측시협중약유2 600다개GABA능양성신경원,타문공분위6군.기중3군위우외수부근(M1-3),령외삼군위우내수복합체변연(LC1-3).GABA능신경원발출적축돌재정개시협적3개신경섬유망중도유분포.상비지하,시협대5-HT항혈청적반응교약,시협신경섬유망중불존재대표5-HT양성반응적조대정맥곡장상섬유,지유일사배렬규칙적세소섬유.매측시협지유위우외수부근적25개신경원정현양성반응,타문적분포위치여부분M3군적GABA능양신경원상동.본문환탐토료5-HT화GABA재조절시각신식시가능발휘적작용[동물학보50(5):770-777,2004].
γ-aminobutyric acid (GABA) and serotonin (5-HT) immunoreactivity in the visual system (compound eyes and optic lobes) of the butterfly Minathyma schrenckii were studied with the CP (Colophony-paraffin) embedding serial section technique and the SP (Streptavidin-peroxidase) immunohistochemical method. Contrary to other insects reported previously, certain photoreceptors of this taxon react with GABA and 5-HT antiserum. In each optic lobe, about 2 600 GABA-like immunoreactive neurons cluster into six groups; three (M1 - 3) are located near the medulla, the other three(LC1 -3) are confined to the lobula complex. Processes from GABA-like neurons supply all the three neuropiles of optic lobes. However, weak 5-HT immunoreactivity in the optic lobes was detected compared to the strong labeling of GABA.The neuropiles are devoid of classical thick 5-HT immunoreactive varicose processes as reported in other insects, and only fine fibres with a regular pattern can be identified. There are only 25 5-HT positive neurons per hemisphere, which share a similar position with M3 GABA-like immunoreactive neurons. The possible involvement of 5-HT and GABA in control of neuronal activity in the visual system is discussed [ Acta Zoologica Sinica 50 (5): 770 - 777, 2004 ].
