时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
11期
2773-2774
,共2页
李妍%宋舒暖%李留法%王中彦%莫凤奎
李妍%宋舒暖%李留法%王中彥%莫鳳奎
리연%송서난%리류법%왕중언%막봉규
高效液相色谱法%清新灵微丸%盐酸小檗碱%黄芩苷
高效液相色譜法%清新靈微汍%鹽痠小檗堿%黃芩苷
고효액상색보법%청신령미환%염산소벽감%황금감
HPLC%Qingxinling pellets%Berberine hydrochloride%Baicalin
目的 建立高效液相色谱法同时测定清新灵微丸中黄芩苷和盐酸小檗碱含量的方法 .方法 采用ODS C_(18)(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇∶水=54∶46(磷酸调pH2.5),检测波长为263 nm,柱温为25 ℃, 流速1.0 ml·min~(-1).结果 盐酸小檗碱和黄芩苷分别在10.0～60.0 μg·ml~(-1)(r=0.999 2)和25.0～150.0 μg·ml~(-1)(r=0.999 1)内线性关系良好,平均加样回收率(n=9)分别为99.24 %和98.66 %.结论 该方法 简便、准确、重复性好,可同时测定清新灵微丸中盐酸小檗碱和黄芩苷的含量.
目的 建立高效液相色譜法同時測定清新靈微汍中黃芩苷和鹽痠小檗堿含量的方法 .方法 採用ODS C_(18)(250 mm×4.6 mm,5μm)色譜柱,流動相為甲醇∶水=54∶46(燐痠調pH2.5),檢測波長為263 nm,柱溫為25 ℃, 流速1.0 ml·min~(-1).結果 鹽痠小檗堿和黃芩苷分彆在10.0～60.0 μg·ml~(-1)(r=0.999 2)和25.0～150.0 μg·ml~(-1)(r=0.999 1)內線性關繫良好,平均加樣迴收率(n=9)分彆為99.24 %和98.66 %.結論 該方法 簡便、準確、重複性好,可同時測定清新靈微汍中鹽痠小檗堿和黃芩苷的含量.
목적 건립고효액상색보법동시측정청신령미환중황금감화염산소벽감함량적방법 .방법 채용ODS C_(18)(250 mm×4.6 mm,5μm)색보주,류동상위갑순∶수=54∶46(린산조pH2.5),검측파장위263 nm,주온위25 ℃, 류속1.0 ml·min~(-1).결과 염산소벽감화황금감분별재10.0～60.0 μg·ml~(-1)(r=0.999 2)화25.0～150.0 μg·ml~(-1)(r=0.999 1)내선성관계량호,평균가양회수솔(n=9)분별위99.24 %화98.66 %.결론 해방법 간편、준학、중복성호,가동시측정청신령미환중염산소벽감화황금감적함량.
Objective To establish the HPLC quantitative method for determination of berberine hydrochloride and baicalin in Qingxinling pellets simutaneously. Methods The analysis was performed on the HPLC Diamonsil - C_(18) column (250 mm x4.6 mm, 5 um) with temperature at 25 C , as mobile phase consisted of methanol - water ( V/V 54:46, pH=2.5) with flow rate of 1.0 ml·min~(-1) and the UV detective wavelength was 263 nm. Results The linearity for berberine hydrochloride and baicalin were 10.0～60.0 ug·ml~(-1) (r =0.999 2) and 25.0～150.0 ug·ml~(-1) (r =0.999 1) respectively. The average recoveries( n = 9) were 99.24% and 98.66% respectively. Conclusion The method is simple, accurate and reproducible, which can be used for the content determination of bererine hydrochloride and baicalin in Qingxinling pellets by HPLC simutaneously.
