CAJ | 학술논문

用水培方法研究了弯囊苔草在不同Cu~(2+)浓度(1、5、25mg·L~(-1))胁迫下的生长特性和部分生理生化指标的变化.结果表明,不同Cu~(2+)度胁迫下,弯囊苔草正常生长,但其生长特性表现出高浓度抑制的相关性,高浓度下没有新生根.弯囊苔草叶片3种抗氧化酶活性都随胁迫时间的延长呈先上升再下降趋势.SOD在处理的第4 d达到峰值,且活性一直显著高于对照(P<0.05),3种Cu~(2+)浓度处理下的峰值大小顺序为5 mg·L~(-1)>25 mg·L~(-1)>1 mg·L~(-1).POD和CAT活性在处理第7d达到峰值,POD活性峰值大小顺序为5mg·L~(-1)>1 mg·L~(-1)>25 mg·L~(-1);CAT活性峰值大小顺序为25 mg·L~(-1)>5 mg·L~(-1)>1 mg·L~(-1).丙二醛(MDA)和可溶性糖含量都是随着胁迫时间的延长而上升,处理结束时的丙二醛(MDA)(r=988 0)和可溶性糖含量(r=0.951 2)与Cu~(2+)浓度表现出正相关性.脯氨酸(Pro)含量随胁迫时间的延长也呈先上升后下降趋势,1mg·L~(-1)和5 mg·L~(-1)C~(2+)度处理下脯氨酸(Pro)含量在处理第4d达到最大值,25 mg·L~(-1)高浓度处理下脯氨酸(Pro)含量在处理第7d达到最大值.叶绿素在5、25 mg·L~(-1)Cu~(2+)度处理下随胁迫时间的延长呈先上升后下降的趋势,1 mg·L~(-1)Cu~(2+)处理下的叶绿素含量不断上升,实验结束时各处理下叶片的叶绿素含量与处理浓度呈负相关.结果表明弯囊苔草能通过调节体内抗氧化酶活性来增强其抗氧化胁迫能力,这为其作为铜污染水体的修复植物提供了可能.
용수배방법연구료만낭태초재불동Cu~(2+)농도(1、5、25mg·L~(-1))협박하적생장특성화부분생리생화지표적변화.결과표명,불동Cu~(2+)도협박하,만낭태초정상생장,단기생장특성표현출고농도억제적상관성,고농도하몰유신생근.만낭태초협편3충항양화매활성도수협박시간적연장정선상승재하강추세.SOD재처리적제4 d체도봉치,차활성일직현저고우대조(P<0.05),3충Cu~(2+)농도처리하적봉치대소순서위5 mg·L~(-1)>25 mg·L~(-1)>1 mg·L~(-1).POD화CAT활성재처리제7d체도봉치,POD활성봉치대소순서위5mg·L~(-1)>1 mg·L~(-1)>25 mg·L~(-1);CAT활성봉치대소순서위25 mg·L~(-1)>5 mg·L~(-1)>1 mg·L~(-1).병이철(MDA)화가용성당함량도시수착협박시간적연장이상승,처리결속시적병이철(MDA)(r=988 0)화가용성당함량(r=0.951 2)여Cu~(2+)농도표현출정상관성.포안산(Pro)함량수협박시간적연장야정선상승후하강추세,1mg·L~(-1)화5 mg·L~(-1)C~(2+)도처리하포안산(Pro)함량재처리제4d체도최대치,25 mg·L~(-1)고농도처리하포안산(Pro)함량재처리제7d체도최대치.협록소재5、25 mg·L~(-1)Cu~(2+)도처리하수협박시간적연장정선상승후하강적추세,1 mg·L~(-1)Cu~(2+)처리하적협록소함량불단상승,실험결속시각처리하협편적협록소함량여처리농도정부상관.결과표명만낭태초능통과조절체내항양화매활성래증강기항양화협박능력,저위기작위동오염수체적수복식물제공료가능.
Effects of Cu~(2+)stress on the growth, activities of antioxidant enzymes, chlorophyU contents, membrane lipid peroxidation, soluble sugar and proline contents in the leaves of Carex dispalata were investigated experimentally with water culture method. The results showed that although Carex dispalata growed normally. the growth characteristics were correlated negatively with increasing Cu~(2+)concentrations, and roots did not regenerate under high Cu~(2+)concentrations. The activities of antioxidant enzymes increased in the beginning and then decreased with increasing stressing time. SOD activities of three treatments(1 mg·L~(-1), 5 mg·L~(-1) and 25 mg·L~(-1)) reached peak value in the 4th day and remained significantly higher than the control treatment(P<0.05) throughout the experiment, and the activities were the highest at 5 mg·L~(-1),followed by 25 mg·L~(-1) and 1 mg·L~(-1), respectively. Both POD and CAT activities reached peaks in the 7th day. The peak value was highest at 5 mg·L~(-1), followed by 1 mg·L~(-1) and 25 mg·L~(-1), respectively, for POD activities, whereas that of CAT was the highest at 25 mg·L~(-1), followed by 5 mg·L~(-1) and 1 mg·L~(-1), respectively. MDA and soluble sugar contents increased with increasing stressing time and both were positively correlated with increasing Cu~(2+)concentrations when the experiment ended r= 0.988 0 and 0.951 2 for MDA and soluble sugar, respectively. Proline contents also increased in the beginning and then decreased with increasing stressing time. Under 1 mg·L~(-1) and 5 mg·L~(-1) Cu~(2+)concentrations, proline contents reached peak value after four-day treatment, while under 25 mg·L~(-1)the peak value reached after seven-day treatment. Similarly, chlorophyU contents increased in the beginning and then decreased with increasing stressing time under 5 mg·L~(-1)and 25 mg·L~(-1) Cu~(2+)concentrations as well, whereas that under 1 mg·L~(-1) Cu~(2+)concentration increased continuously. The chlorophyU contents were all