CAJ | 학술논문

边合成边测序的高通量基因测序技术存在一个相位问题,即碱基合成的先后顺序会出现"超前"和"延迟".针对此问题,结合Markov过程的方法提出一种回归分析,在建立有关相位"超前"与"延迟"问题的荧光强度数据基础上,构造出相位校正矩阵,解决高通量基因测序中的相位问题.实验结果表明,采用提出的相位校正方法,达到对碱基合成时出现的相位"超前"和"延迟"问题的校正.
변합성변측서적고통량기인측서기술존재일개상위문제,즉감기합성적선후순서회출현"초전"화"연지".침대차문제,결합Markov과정적방법제출일충회귀분석,재건립유관상위"초전"여"연지"문제적형광강도수거기출상,구조출상위교정구진,해결고통량기인측서중적상위문제.실험결과표명,채용제출적상위교정방법,체도대감기합성시출현적상위"초전"화"연지"문제적교정.
The high-throughput genome sequencing has a phase problem, i.e."exceeding" or "delaying", which appears in the order of bases synthesizing. Combined with Markov process, a method of regression analysis was proposed. Based on the constructed data of fluorescence intensity, being correlated with phase " exceeding" and " delaying" questions, a phase emendation matrix was set up to resolve the phase problem. The experimental result shows the method can eliminate the phase "exceeding" or "delaying".