CAJ | 학술논문

目的探讨单纯疱疹病毒胸苷激酶(HSV-TK)基因联合羟基无环鸟苷(GCV)对膀胱癌细胞的旁观杀伤作用及其机制。方法将转染HSV-TK基因的膀胱癌细胞(BIU87-TK)分别与人膀胱癌细胞(BIU87、T24)和鼠膀胱癌细胞(T739)混合培养。在GCV作用下，观测细胞的存活情况及BIU87-TK条件培养液和细胞死亡裂解对共培养细胞的影响。结果当BIU87-TK细胞占50%时，共培养细胞对GCV的敏感性与100%BIU87-TK细胞一致。表明每个表达HSV-TK基因的细胞能杀死至少一个不含HSV-TK基因的膀胱癌细胞。BIU87-TK细胞的条件培养液对BIU87亲本细胞的生长无影响。G418诱导的细胞裂解对与其共培养的BIU87-TK细胞无旁观毒性作用。结论 HSV-TK/GCV对膀胱癌细胞产生的“旁观者”效应是该系统特异性的毒性作用。“旁观者”效应不仅可在人膀胱癌不同细胞株之间产生，而且不局限于某一种属的膀胱癌细胞之间。
목적 탐토단순포진병독흉감격매(HSV-TK)기인연합간기무배조감(GCV)대방광암세포적방관살상작용급기궤제。 방법 장전염HSV-TK기인적방광암세포(BIU87-TK)분별여인방광암세포(BIU87、T24)화서방광암세포(T739)혼합배양。재GCV작용하，관측세포적존활정황급BIU87-TK조건배양액화세포사망렬해대공배양세포적영향。 결과 당BIU87-TK세포점50%시，공배양세포대GCV적민감성여100%BIU87-TK세포일치。표명매개표체HSV-TK기인적세포능살사지소일개불함HSV-TK기인적방광암세포。BIU87-TK세포적조건배양액대BIU87친본세포적생장무영향。G418유도적세포렬해대여기공배양적BIU87-TK세포무방관독성작용。 결론 HSV-TK/GCV대방광암세포산생적“방관자”효응시해계통특이성적독성작용。“방관자”효응불부가재인방광암불동세포주지간산생，이차불국한우모일충속적방광암세포지간。
Objective To investigate the bystander tumoricidal effectmediated by HSV-TK/GCV system on the bladder cancer cells and the possible mechanisms of bystander effects. Methods HSV-TK gene-transduced BIU 87 cells (BIU 87-TK) were cocultured with the human bladder cancer cells (BIU 87,T 24)and a mouse bladder carcinoma cell line (T 739).In the presence of ganciclovir,the cell survival rate was monitoried.In addition the influence of the conditioned media from cultures of BIU 87-TK and G 418-induced cell lysis on cocultured cells was studied. Results When the proportion of BIU 87-TK cells is about 50%,the cocultures that contained 50% BIU 87-TK cells were as sensitive to GCV as cultures containing 100% BIU 87-TK cells.This indicated that one cell expressing HSV-TK gene was capable of me-diating death to at least one tumor cell carrying no HSV-TK genes.The conditioned media from cultures of BIU 87-TK cells in the presence of GCV exhibited no effects on survival of the BIU 87 parental cells.G 418-induced cell lysis did not mediate bystander cytocidal effect on the co-cultured BIU 87-TK cells. Conclusions The bystander effect among the bladder carcinoma lines in this study,which is not mediated by the cytotoxic effects of cell lysis or by a soluble factor(r) by culturing HSV-TK cells,is specific for cytotoxicity induced by HSV-TK/GCV system.In addition,the bystander effect can function between cells of diffe-rent human bladder tumors,and is not restricted to bladder cancer cell of a particular specie.