CAJ | 학술논문

目的探讨实时荧光定量PCR检测不同血清标志物模式的乙型肝炎患者的血清HBV DNA含量的特点.方法应用ELISA对512例病人血清中乙型肝炎血清标志物进行检测,同时用实时荧光定量PCR检测标本中HBV DNA的含量,分别统计每组的HBV DNA阳性例数及其阳性率,用u检验进行统计学处理.结果 HBsAg(+)/HBeAg(+)/抗HBc(+)组168例,HBV DNA阳性161例,占95.8%;HBsAg(+)/抗HBe(+)/抗HBc(+)组163例,HBVDNA阳性51例,占31.3%;HBsAg(+)/抗HBc(+)组181例,HBVDNA阳性94例占51.9%;三组之间互相比较差异均有显著性(P<0.001).结论 HBsAg(+)/HBeAg(+)/抗HBc(+)组中HBV复制最活跃,实时荧光定量PCR检测HBV DNA比血清标志更能反映乙型肝炎病毒存在与复制情况.
목적 탐토실시형광정량PCR검측불동혈청표지물모식적을형간염환자적혈청HBV DNA함량적특점.방법 응용ELISA대512례병인혈청중을형간염혈청표지물진행검측,동시용실시형광정량PCR검측표본중HBV DNA적함량,분별통계매조적HBV DNA양성례수급기양성솔,용u검험진행통계학처리.결과 HBsAg(+)/HBeAg(+)/항HBc(+)조168례,HBV DNA양성161례,점95.8%;HBsAg(+)/항HBe(+)/항HBc(+)조163례,HBVDNA양성51례,점31.3%;HBsAg(+)/항HBc(+)조181례,HBVDNA양성94례점51.9%;삼조지간호상비교차이균유현저성(P<0.001).결론 HBsAg(+)/HBeAg(+)/항HBc(+)조중HBV복제최활약,실시형광정량PCR검측HBV DNA비혈청표지경능반영을형간염병독존재여복제정황.
Objective To investigate the characteristics of serum HBV DNA content through detecting the diffent serum markers of hepatitis B using the method of the real-time fluorescence quantitative PCR.Methods 512 cases of hepatitis B serum markers were detected with ELISA, and simultaneously use the real-time fluorescence quantitative PCR to detect HBV DNA content in the serum. Statistics for each group of HBV DNA-positive cases and its positive rate (%) were separately done.u test were used for the statistical treannent.Results HBsAg (+) / HBeAg (+) / anti-HBc (+) group has 168 cases, HBV DNA positive has 61 cases (95.8%); HBsAg (+) /anti-HBe (+) /anti-HBc (+) group has 163 cases,HBV DNA positive has 51 cases (31.3%); HBsAg (+) / anti-HBc (+) group has 181 cases,HBV DNA positive has 94 cases (51.9%). Comparisons among three.groups were significantly different (P<0.001).Conclusions The reproduction of HBV in the HBsAg (+)/ HBeAg (+)/ anti-HBc (+) group were the most active.Real-time fluorescence quantitative PCR detection of HBV DNA is the better method to reflect the existence and the reproduction of hepatitis B virus than Serological markers.