CAJ | 학술논문

目的以老年性痴呆(AD)大鼠为研究对象,应用蛋白质组学方法在血清中筛选与AD密切相关的蛋白质,为研究AD的发病机制提供理论支持.方法通过Morris水迷宫记忆行为学筛选符合痴呆标准的AD大鼠模型,收集AD组大鼠及假手术组大鼠的血清后,去除血清中的高丰度蛋白质并进行蛋白质浓度的测定,之后进行双向凝胶电泳,挑选差异最为明显的蛋白质点进行肽质量指纹图谱分析,经数据库查询.鉴定差异蛋白质.结果经观察二维凝胶电泳图谱后,发现AD组大鼠与假手术组大鼠的血清中共有10个差异明显的蛋白质点,选取了2个最为明显的蛋白质点进行鉴定,分别为:血浆视黄醇结合蛋白前体,补体成分4,基因2(C4b).结论 C4b是新发现的与AD相关的蛋白质,有可能是潜在的AD标志物,有助于更深刻地理解AD的发病机制.
목적 이노년성치태(AD)대서위연구대상,응용단백질조학방법재혈청중사선여AD밀절상관적단백질,위연구AD적발병궤제제공이론지지.방법 통과Morris수미궁기억행위학사선부합치태표준적AD대서모형,수집AD조대서급가수술조대서적혈청후,거제혈청중적고봉도단백질병진행단백질농도적측정,지후진행쌍향응효전영,도선차이최위명현적단백질점진행태질량지문도보분석,경수거고사순.감정차이단백질.결과 경관찰이유응효전영도보후,발현AD조대서여가수술조대서적혈청중공유10개차이명현적단백질점,선취료2개최위명현적단백질점진행감정,분별위:혈장시황순결합단백전체,보체성분4,기인2(C4b).결론 C4b시신발현적여AD상관적단백질,유가능시잠재적AD표지물,유조우경심각지리해AD적발병궤제.
Objective Alzheimer's disease(AD) is a common and devastating disease and there is no readily available biomarker to aid diagnosis or to monitor disease progression. This study is to further understand the pathogenesis of Alzheimer's disease and seek new biomarkers for AD. Methods AD rats made by Aβ1-40 were assessed using the Morris water maze method. Sera of two groups were collected and albumin, IgG and sodium chloride were removed from the serum before concentration. Two-dimensional electrophoresis(2-DE) was performed on serum protein. Altered proteins were identified by matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Results Compared with untreated samples, 10 altered proteins were found through 2-DE and all of them were down-regulated of which 2 were identified by MALDI-TOF-MS to be plasma retinol-binding protein precursor and complement component 4, gene 2 (C4b). Conclusion C4b may be the biomarker of AD and it may be helpful to understand the pathogenesis of AD.