CAJ | 학술논문

背景先前的系列研究表明,姜黄素可以诱导体外培养的兔视网膜色素上皮(RPE)细胞凋亡,抑制其RPE细胞的增生,且在玻璃体内应用后不良反应较小,具有防治增生性玻璃体视网膜病变(PVR)的潜在价值.目的探讨姜黄素玻璃体内注射对RPE细胞诱导的兔眼PVR模型的防治效果.方法新西兰白兔20只40只眼,所有兔眼玻璃体注射前先抽取0.2ml玻璃体液,然后在兔眼玻璃体内注射0.1ml(2×10 6)同种RPE细胞,每只兔随机选取1只眼立即注入1mg/L的姜黄素0.1ml作为姜黄素组(20只眼),对侧眼注入等量的含质量分数0.5‰DMSO的生理盐水作为对照组(20只眼).注药后1、3、7、14、21、28 d裂隙灯显微镜下观察角膜、房水、晶状体的透明度及眼前节炎症反应情况;使用间接检眼镜、眼底彩色照相和B型超声检查玻璃体视网膜情况.以视网膜脱离发生眼数作为检测指标,评价姜黄素对PVR的防治效果.结果玻璃体注药后1d、3d所有兔眼发生眼前节炎症反应,玻璃体轻中度混浊,但未见增生条带及视网膜脱离.玻璃体注药后7d,所有兔眼前节炎症反应基本消退,对照组14只眼(75%)玻璃体出现增生条带,姜黄素组2只眼(10%)玻璃体内出现增生条带,差异有统计学意义(P<0.01),但2组均未见视网膜脱离.注药后14d,对照组11只眼(55%)出现视网膜脱离,姜黄素组2只眼(10%)出现视网膜脱离,差异有统计学意义(P<0.01);注药后21d,对照组16只眼(80%)出现视网膜脱离,姜黄素组3只眼(15%)出现视网膜脱离;注药后28d,对照组19只眼(95%)出现视网膜脱离,姜黄索组3只眼(15%)出现视网膜脱离,差异有统计学意义(P<0.01).结论姜黄素玻璃体腔内注射可以有效预防RPE细胞诱导的兔眼实验性PVR的发生发展.
배경 선전적계렬연구표명,강황소가이유도체외배양적토시망막색소상피(RPE)세포조망,억제기RPE세포적증생,차재파리체내응용후불량반응교소,구유방치증생성파리체시망막병변(PVR)적잠재개치.목적 탐토강황소파리체내주사대RPE세포유도적토안PVR모형적방치효과.방법신서란백토20지40지안,소유토안파리체주사전선추취0.2ml파리체액,연후재토안파리체내주사0.1ml(2×10 6)동충RPE세포,매지토수궤선취1지안립즉주입1mg/L적강황소0.1ml작위강황소조(20지안),대측안주입등량적함질량분수0.5‰DMSO적생리염수작위대조조(20지안).주약후1、3、7、14、21、28 d렬극등현미경하관찰각막、방수、정상체적투명도급안전절염증반응정황;사용간접검안경、안저채색조상화B형초성검사파리체시망막정황.이시망막탈리발생안수작위검측지표,평개강황소대PVR적방치효과.결과 파리체주약후1d、3d소유토안발생안전절염증반응,파리체경중도혼탁,단미견증생조대급시망막탈리.파리체주약후7d,소유토안전절염증반응기본소퇴,대조조14지안(75%)파리체출현증생조대,강황소조2지안(10%)파리체내출현증생조대,차이유통계학의의(P<0.01),단2조균미견시망막탈리.주약후14d,대조조11지안(55%)출현시망막탈리,강황소조2지안(10%)출현시망막탈리,차이유통계학의의(P<0.01);주약후21d,대조조16지안(80%)출현시망막탈리,강황소조3지안(15%)출현시망막탈리;주약후28d,대조조19지안(95%)출현시망막탈리,강황색조3지안(15%)출현시망막탈리,차이유통계학의의(P<0.01).결론 강황소파리체강내주사가이유효예방RPE세포유도적토안실험성PVR적발생발전.
Background Our previous study demonstrated that curcumin can induce the apoptosis of retinal pigment epithelial (RPE) cells and herein inhibit the proliferation of RPE cells,and it is proved that the intravitreous injection of 0.1mg curcumin has less adverse effect to ocular tissue, inferring a good applicative prospect in clinic. Objective The goal of this experiment was to evaluate the effectiveness of curcumin on the prevention and treatment of experimental proliferative vitreoretinopathy (PVR). Methods PVR models were induced by injection of 0.1ml RPE cells (containing 2×106 cells) into vitreous cavity in 40 eyes of 20 healthy and mature New Zealand albino rabbits.0. 1ml curcumin(0. 1 mg) was then injected into lateral eye of each model rabbit immediately following the injection of RPE cells,and the equal volume of normal saline solution containing 0. 5‰ DMSO was injected into the fellow eye of each model rabbit as controls. On 1,3,7,14,21 and 28 days after injection, the changes of cornea, aqueous humor, lens, vitreous and fundus were examined and recorded by slit lamp biomicroscope, indirect ophthalmoscope,fundus color camera and B-type ultrasonograph to evaluate the inflammatory response. The incidence rate of retinal detachment was calculated and compared between curcumin group and control group. Results The inflammatory reaction in anterior chamber and misty opacity in vitreous were found from 1 day through 3 days after injection, but no obvious proliferative strap and retinal detachment in all of the experimental eyes. On the 7th day after injection, inflammatory reaction was extinct in the anterior chamber of rabbit eyes, and proliferative strap occurred in 14 eyes(75% ) in the control group but only 2 eyes (10% ) in curcumin group,showing significant difference between these two groups (P<0. 01). No retinal detachment was seen in both the two groups. On 14,21 and 28 days after injection, the incidence rate of retinal detachment was 55% ,80% ,95% respectively in control group and that of curcumin group was 10% ,15% ,15% respectively,presenting considerably differences between two groups (P<0. 01, P<0. 01 ,P<0. 01 ). Conclusion Injection of curcumin into vitreous cavity can effectively inhibit the occurrence and development of PVR in rabbit.