CAJ | 학술논문

目的探讨人骨髓间充质干细胞(MSC)体外分化为角膜上皮细胞的可行性.方法实验研究.分别取第3代人MSC和自行培养的第3代人角膜基质细胞共同培养1周,实验组在Transwell共培养体系中培养,对照组不放置Transwell小室培养.1周后,观察实验组和对照组中人MSC光镜特征、间接免疫细胞化学染色和电镜结构,对被诱导分化的细胞进行综合鉴别.结果第3代人MSC和人角膜基质细胞在体外培养条件下均能够较快贴壁生长.两种细胞共同培养1周后,可见部分细胞形态上呈上皮细胞特征,单克隆抗体AE1染色呈阳性,电镜下可见微绒毛、桥粒和张力丝等典型上皮细胞结构特征.结论体外培养的人MSC在人角膜基质细胞的诱导下可能会分化为人角膜上皮样细胞.
목적 탐토인골수간충질간세포(MSC)체외분화위각막상피세포적가행성.방법 실험연구.분별취제3대인MSC화자행배양적제3대인각막기질세포공동배양1주,실험조재Transwell공배양체계중배양,대조조불방치Transwell소실배양.1주후,관찰실험조화대조조중인MSC광경특정、간접면역세포화학염색화전경결구,대피유도분화적세포진행종합감별.결과 제3대인MSC화인각막기질세포재체외배양조건하균능구교쾌첩벽생장.량충세포공동배양1주후,가견부분세포형태상정상피세포특정,단극륭항체AE1염색정양성,전경하가견미융모、교립화장력사등전형상피세포결구특정.결론 체외배양적인MSC재인각막기질세포적유도하가능회분화위인각막상피양세포.
Objective To explore the feasibility of the transdifferentiation of human mesenchymal stem cell (MSC) into corneal epithelial cells in vitro. Methods Experimental study.The third passage human MSC and the third passage corneal stromal fibroblasts were co-cultured in a Transwell culture system for 1 week. The human MSC were observed and identified by light microscopy, scanning and transmission electron microscopy, and comprehensive indirect immunocytochemistry staining in the induced and control groups. Results Both the third passage human MSC and the third passage corneal stromal fibroblast cells could adhere and grow rapidly.After co-culture with corneal stromal fibroblasts for 1 week, the morphology of some induced human MSC changed into epithelial-like cells. Some induced human MSC showed positive staining for monoclonal antibody AE1, which were typical epithelium cells with a large quantity of microvilli,desmosomes and tonofilaments that were observed under electron microcopy. Conclusion The data suggests that human MSC may have the potential to transdifferentiate into human corneal epithelial-like cells in vitro.