国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2010年
15期
1883-1885
,共3页
汤南%徐招玉%梁伟亮%吴劲东
湯南%徐招玉%樑偉亮%吳勁東
탕남%서초옥%량위량%오경동
布洛芬%固体分散体%反相高效液相色谱法%含量测定
佈洛芬%固體分散體%反相高效液相色譜法%含量測定
포락분%고체분산체%반상고효액상색보법%함량측정
Ibuprofen%Solid dispersion%RP-HPLC%Assay
目的 建立反相高效液相色谱法测定布洛芬-聚乙二醇6000固体分散体中布洛芬的含量.方法 色谱柱为Shimadzu C18柱,流动相为0.02 mol·L-1醋酸钠(冰醋酸调节pH至3.0±0.1)-乙腈(40:60),流速1.0ml·min-1,检测波长为264nm,进样量为20μl.结果 布洛芬质量浓度在0.051～1.02 mg·ml-1范围内与相应峰面积呈良好线性关系(r=0.999 9,n=6),平均加样回收率为98.40%,RSD为0.92%(n=9).结论 所用方法准确、简便、快速,适用于布洛芬-聚乙二醇6000固体分散体中布洛芬的含量测定.
目的 建立反相高效液相色譜法測定佈洛芬-聚乙二醇6000固體分散體中佈洛芬的含量.方法 色譜柱為Shimadzu C18柱,流動相為0.02 mol·L-1醋痠鈉(冰醋痠調節pH至3.0±0.1)-乙腈(40:60),流速1.0ml·min-1,檢測波長為264nm,進樣量為20μl.結果 佈洛芬質量濃度在0.051～1.02 mg·ml-1範圍內與相應峰麵積呈良好線性關繫(r=0.999 9,n=6),平均加樣迴收率為98.40%,RSD為0.92%(n=9).結論 所用方法準確、簡便、快速,適用于佈洛芬-聚乙二醇6000固體分散體中佈洛芬的含量測定.
목적 건립반상고효액상색보법측정포락분-취을이순6000고체분산체중포락분적함량.방법 색보주위Shimadzu C18주,류동상위0.02 mol·L-1작산납(빙작산조절pH지3.0±0.1)-을정(40:60),류속1.0ml·min-1,검측파장위264nm,진양량위20μl.결과 포락분질량농도재0.051～1.02 mg·ml-1범위내여상응봉면적정량호선성관계(r=0.999 9,n=6),평균가양회수솔위98.40%,RSD위0.92%(n=9).결론 소용방법준학、간편、쾌속,괄용우포락분-취을이순6000고체분산체중포락분적함량측정.
Objective To apply RP-HPLC to determine the contents of ibuprofen in ibuprofen-PEG6000 solid dispersion. Methods The separation was performed on a Shimadzu C18 column with UV detection at 264 nm, and the mobile phase consisted of 0.02 moLL-1 sodium acetate (adjusted to pH 3.0 ± 0.1 with acetic acid) -acetonitrile (40:60). The flow rate was 1.0 ml.min-1. The sample was injected at 20 μl.Results The calibration curve was linear (r = 0.999 9) within the range of 0.051 mg.ml-1 to 1.02 mg.ml-1 for ibuprofen. The average rate of retrieval was 98.40%, RSD was 0.92% (n = 9). Conclusions This method is accurate, simple, quick, and suitable for the quality control of Ibuprofen-PEG6000 solid dispersion.
