CAJ | 학술논문

目的探讨糖尿病大鼠颈动脉窦隔离灌注异丙酚对血浆内皮素(ET)及一氧化氮(NO)水平的影响.方法健康清洁级成年雄性Wistar大鼠,体重180 ～ 220 g,采用腹腔注射链脲佐菌素30mg/kg的方法制备糖尿病模型.取糖尿病模型制备成功的大鼠36只,采用随机数字表法,将其随机分为3组(n=12):对照组(DC组)、低浓度异丙酚组(DP1组)和高浓度异丙酚组(DP2组).另取非糖尿病大鼠36只,采用随机数字表法,将其随机分为3组(n=12):对照组(NC组)、低浓度异丙酚组(NP组)和高浓度异丙酚组(NP2组).颈动脉窦隔离灌流K-H液(对照组)、含50 μmol/L异丙酚的K-H液(低浓度异丙酚组)和含100 μmol/L异丙酚的K-H液(高浓度异丙酚组),30 min后取股动脉血样,测定血浆ET和NO浓度.结果与DC组比较,DP1组和DP2组血浆ET浓度降低,NO浓度升高(P＜0.05)；与DP1组比较,DP2组血浆ET浓度降低,NO浓度升高,NP1组血浆ET浓度升高,NO浓度降低(P＜0.05)；与DP2组比较,NP2组血浆ET浓度升高,NO浓度降低(P＜0.05).结论异丙酚可通过对颈动脉窦压力感受器的局部作用降低糖尿病和非糖尿病大鼠血浆ET水平,升高NO水平,且对糖尿病大鼠的效应更强.
목적 탐토당뇨병대서경동맥두격리관주이병분대혈장내피소(ET)급일양화담(NO)수평적영향.방법 건강청길급성년웅성Wistar대서,체중180 ～ 220 g,채용복강주사련뇨좌균소30mg/kg적방법제비당뇨병모형.취당뇨병모형제비성공적대서36지,채용수궤수자표법,장기수궤분위3조(n=12):대조조(DC조)、저농도이병분조(DP1조)화고농도이병분조(DP2조).령취비당뇨병대서36지,채용수궤수자표법,장기수궤분위3조(n=12):대조조(NC조)、저농도이병분조(NP조)화고농도이병분조(NP2조).경동맥두격리관류K-H액(대조조)、함50 μmol/L이병분적K-H액(저농도이병분조)화함100 μmol/L이병분적K-H액(고농도이병분조),30 min후취고동맥혈양,측정혈장ET화NO농도.결과 여DC조비교,DP1조화DP2조혈장ET농도강저,NO농도승고(P＜0.05)；여DP1조비교,DP2조혈장ET농도강저,NO농도승고,NP1조혈장ET농도승고,NO농도강저(P＜0.05)；여DP2조비교,NP2조혈장ET농도승고,NO농도강저(P＜0.05).결론 이병분가통과대경동맥두압력감수기적국부작용강저당뇨병화비당뇨병대서혈장ET수평,승고NO수평,차대당뇨병대서적효응경강.
Objective To investigate the effect of isolated carotid sinus perfusion with propofol on plasma endothelin and nitric oxide levels in diabetic rats.Methods Healthy adult Wistar rats weighing 180-220 g were used in this study.Diabetes mellitus was induced by intraperitoneal streptozotocin 30 mg/kg and confirmed by blood glucose ≥ 16.7 mmol/L.Thirty-six rats in which diabetes mellitus model was successfully established were randomly divided into 3 groups ( n =12):control group (group DC) ;low dose propofol group (group DP1 ) and high dose propofol group (group DP2 ).Another 36 non-diabetic rats were also randomly divided into 3 group ( n =12 each):control group (group NC) ; low dose propofol group group (group NP1 ) and high dose propofol (group NP2 ).After 30 min isolated carotic sinus perfusion with K-H solution (control groups),50 μmol/L propofol in K-H solution (low dose propofol groups) or 100 μmol/L propofol in K-H solution (high dose propofol groups),blood samples were taken for determination of plasma endothelin and nitric oxide concentrations.Results The plasma endothelin concentration was lower and nitric oxide concentration higher in groups DP1 and DP2 than in group DC,and in group DP1 than in group DP2 ( P ＜ 0.05).The plasma endothelin concentration was higher and nitric oxide concentration lower in group NPi than in group DP1,and in group NP2 than in group DP2 ( P ＜ 0.05 ).Conclusion Propofol can decrease endothelin level and increase nitric oxide level in diabetic or non-diabetic rats through local effect on carotid sinus,and the effect is stronger in diabetic rats than in non-diabetic rats.