中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
12期
2134-2135
,共2页
杨小华%胡明政%秦磊%钱海鑫
楊小華%鬍明政%秦磊%錢海鑫
양소화%호명정%진뢰%전해흠
肝移植%缺血%再灌注损伤%17β-雌二醇
肝移植%缺血%再灌註損傷%17β-雌二醇
간이식%결혈%재관주손상%17β-자이순
Liver transplantation%Ischemia%Reperfusion injury%17β-Estradiol
目的 观察17β-雌二醇对大鼠肝移植缺血再灌注损伤的保护作用.方法 将36只健康雄性SD大鼠随机分成3组:假手术组(Sham组)、缺血再灌注组(I/R组)、缺血再灌注+雌二醇组(I/R+ E2组),I/R组与I/R+ E2组改良二袖套法行肝移植术.术后6h处死受体,检测谷丙转氨酶(ALT)、谷草转氨酶(AST)及丙二醛(MDA)含量,电镜下观察肝组织的超微结构,检测肝组织肿瘤坏死因子-α (TNF-α)的表达.结果 I/R组、I/R+ E2组ALT( 950.83±65.20)、(271.67±22.29) U/L,AST(2436.67±89.70)、(1152.83±85.37) U/L,MDA (0.63±0.03)、(0.40±0.02)nmol/mg,较Sham组(60.32±7.31) U/L、(38.12±4.33) U/L、(0.30±0.04) nmol/mg高(P＜0.05),I/R组均高于I/R+ E2组(P＜0.05).TNF-α在I/R组的表达最明显,I/R+ E2组的阳性表达少于I/R组.电镜下I/R组的肝细胞损伤显著重于I/R+ E2组.结论 雌激素对大鼠肝移植缺血再灌注损伤有保护作用,其机制可能与雌激素降低肝脏I/R时TNF-α和MDA有关.
目的 觀察17β-雌二醇對大鼠肝移植缺血再灌註損傷的保護作用.方法 將36隻健康雄性SD大鼠隨機分成3組:假手術組(Sham組)、缺血再灌註組(I/R組)、缺血再灌註+雌二醇組(I/R+ E2組),I/R組與I/R+ E2組改良二袖套法行肝移植術.術後6h處死受體,檢測穀丙轉氨酶(ALT)、穀草轉氨酶(AST)及丙二醛(MDA)含量,電鏡下觀察肝組織的超微結構,檢測肝組織腫瘤壞死因子-α (TNF-α)的錶達.結果 I/R組、I/R+ E2組ALT( 950.83±65.20)、(271.67±22.29) U/L,AST(2436.67±89.70)、(1152.83±85.37) U/L,MDA (0.63±0.03)、(0.40±0.02)nmol/mg,較Sham組(60.32±7.31) U/L、(38.12±4.33) U/L、(0.30±0.04) nmol/mg高(P＜0.05),I/R組均高于I/R+ E2組(P＜0.05).TNF-α在I/R組的錶達最明顯,I/R+ E2組的暘性錶達少于I/R組.電鏡下I/R組的肝細胞損傷顯著重于I/R+ E2組.結論 雌激素對大鼠肝移植缺血再灌註損傷有保護作用,其機製可能與雌激素降低肝髒I/R時TNF-α和MDA有關.
목적 관찰17β-자이순대대서간이식결혈재관주손상적보호작용.방법 장36지건강웅성SD대서수궤분성3조:가수술조(Sham조)、결혈재관주조(I/R조)、결혈재관주+자이순조(I/R+ E2조),I/R조여I/R+ E2조개량이수투법행간이식술.술후6h처사수체,검측곡병전안매(ALT)、곡초전안매(AST)급병이철(MDA)함량,전경하관찰간조직적초미결구,검측간조직종류배사인자-α (TNF-α)적표체.결과 I/R조、I/R+ E2조ALT( 950.83±65.20)、(271.67±22.29) U/L,AST(2436.67±89.70)、(1152.83±85.37) U/L,MDA (0.63±0.03)、(0.40±0.02)nmol/mg,교Sham조(60.32±7.31) U/L、(38.12±4.33) U/L、(0.30±0.04) nmol/mg고(P＜0.05),I/R조균고우I/R+ E2조(P＜0.05).TNF-α재I/R조적표체최명현,I/R+ E2조적양성표체소우I/R조.전경하I/R조적간세포손상현저중우I/R+ E2조.결론 자격소대대서간이식결혈재관주손상유보호작용,기궤제가능여자격소강저간장I/R시TNF-α화MDA유관.
Objective To observe the effect of 17β-estradiol (E2) on ischemia-reperfusion injury (IRI) in rat orthotopic liver transplantation.Methods Thirty-six SD male rats were randomized into three groups:sham group,I/R group,I/R + E2 group.The rate in I/R group and I/R + E2 group were subjected to liver transplantation by modified two-cuffed methods.Six h after reperfusion,the recipients were sacrificed.The levels of ALT,AST and MDA were measured.Liver ultrastructures were observed under the electron microscopy.The levels of tumor necrosis factor ( TNF)-α were detected by using immuneohistochemistry.Results In I/R group and I/R + E2 group,the levels of ALT ( 950.83 ± 65.20 ) U/L and (271.67 ±22.29) U/L,AST (2436.67 ±89.70) U/L and ( 1152.83 ±85.37) U/L and MDA (0.63 ±0.03) nmol/mg and (0.40 ±0.02) nmol/mg protein were significant higher than in sham group (60.32 ±7.31 ) U/L,(38.12 ±4.33 ) U/L,and (0.30 ±0.04) nmol/mg protein,respectively ( all P ＜0.05 ),especially higher in I/R group.Immunohistochemistry revealed the the levels of TNF-α were highest in I/R group,and those of TNF-α in I/R + E2 group were lower than in I/R group.Under the electron microscopy,the injury of hepatocytes in I/R group was significantly severer than in I/R + E2 group.Conclusion Estradiol can decrease ischemia-reperfusion injury in rat liver transplantation probably by decreasing the levels of TNF-α and MDA.
