CAJ | 학술논문

目的探讨支气管哮喘大鼠肺内神经营养因子水平的变化,及其与气道神经可塑性改变和气道高反应性间的关系. 方法雄性SD大鼠40只按随机数字表法分为对照组、哮喘组、神经生长因子(NGF)+脑源性神经营养因子(BDNF)干预组和anti-NGF+anti-BDNF干预组(每组各10只),后三组通过鸡卵蛋白致敏激发建立哮喘模型并给予相应的干预.造模后第8周进行气道阻力测定,并留取左肺行HE染色及免疫组化染色检测肺组织中神经营养因子如NGF、BDNF的表达水平,RT-PCR法检测气道突触囊泡素(SYN)mRNA和神经丝(NF)mRNA的表达水平. 结果哮喘组及NGF+BDNF干预组大鼠肺组织内可见大量炎症细胞浸润.哮喘组大鼠肺组织中NGF及BDNF的表达水平均明显高于对照组及anti-NGF+anti-BDNF干预组,差异有统计学意义(P＜0.05),而NGF+BDNF干预组肺组织中NGF及BDNF的表达水平明显高于哮喘组,差异亦有统计学意义 (P＜0.05).哮喘组及NGF+BDNF干预组较对照组的气道反应性增高,气道感觉神经元SYN mRNA及NF mRNA的表达水平也明显增高,差异均有统计学意义(P＜0.05).哮喘组大鼠肺组织中NGF及BDNF浓度与气道SYN表达呈正相关关系(r=0.889,P＜0.05；r=0.985,P＜0.05),与NF表达呈正相关关系(r=0.956,P＜0.05；r=0.927,P＜0.05),与气道反应性也呈正相关关系(r=0.938,P＜0.05；r=0.906,P＜0.05). 结论 NGF及BDNF可能参与了支气管哮喘大鼠气道神经可塑性改变的调节过程,从而导致气道高反应性的发生.
목적 탐토지기관효천대서폐내신경영양인자수평적변화,급기여기도신경가소성개변화기도고반응성간적관계. 방법 웅성SD대서40지안수궤수자표법분위대조조、효천조、신경생장인자(NGF)+뇌원성신경영양인자(BDNF)간예조화anti-NGF+anti-BDNF간예조(매조각10지),후삼조통과계란단백치민격발건립효천모형병급여상응적간예.조모후제8주진행기도조력측정,병류취좌폐행HE염색급면역조화염색검측폐조직중신경영양인자여NGF、BDNF적표체수평,RT-PCR법검측기도돌촉낭포소(SYN)mRNA화신경사(NF)mRNA적표체수평. 결과 효천조급NGF+BDNF간예조대서폐조직내가견대량염증세포침윤.효천조대서폐조직중NGF급BDNF적표체수평균명현고우대조조급anti-NGF+anti-BDNF간예조,차이유통계학의의(P＜0.05),이NGF+BDNF간예조폐조직중NGF급BDNF적표체수평명현고우효천조,차이역유통계학의의 (P＜0.05).효천조급NGF+BDNF간예조교대조조적기도반응성증고,기도감각신경원SYN mRNA급NF mRNA적표체수평야명현증고,차이균유통계학의의(P＜0.05).효천조대서폐조직중NGF급BDNF농도여기도SYN표체정정상관관계(r=0.889,P＜0.05；r=0.985,P＜0.05),여NF표체정정상관관계(r=0.956,P＜0.05；r=0.927,P＜0.05),여기도반응성야정정상관관계(r=0.938,P＜0.05；r=0.906,P＜0.05). 결론 NGF급BDNF가능삼여료지기관효천대서기도신경가소성개변적조절과정,종이도치기도고반응성적발생.
Objective To investigate the effect of change in neurotrophins level in the bronchial asthma rats' lung on airway hyperresponsiveness and airway neural plasticity. Methods A total of forty male SD rats were randomly divided into 4 groups with 10 rats in each group: control group,asthmatic group, NGF+BDNF prevention group and anti-NGF+anti-BDNF prevention group. The asthmatic model was established by inhalation and injection of ovalbumin. The airway responsiveness was measured after 8 weeks.The bronchial inflammation was assessed by HE staining,and nerve growth factor and brain derived neurotrophic factor expressions of left lung were assayed by the immunohistochemistry staining.Then the expressions of synaptophysin and neurofilament were detected by RT-PCR. Results Compared with the control group, the lung tissue of the asthma group and NGF+BDNF prevention group had more infiltrating inflammatory cells; The expressions of NGF and BDNF were higher in the asthma group and NGF+BDNF prevention group than those in the control group and anti-NGF+anti-BDNF prevention group (P＜0.05),and significantly higher in the NGF+BDNF prevention group than those in the asthma group (P＜0.05).Both the airway responsiveness and the levels of SYN mRNA and NF mRNA in the lung tissues were significantly higher in the asthma group and NGF+BDNF prevention group than those in the control group (P＜0.05).In asthma group,the expressions of NGF and BDNF were positively related to the expressions of SYN (r=0.889,P＜0.05; r=0.985,P＜0.05)and NF(r=0.956,P＜0.05; r=0.927,P＜0.05),and also positively related to the airway hyperresponsiveness (r=0.938,P＜0.05; r=0.906,P＜0.05). Conclusion NGF and BDNF might be involved in rat airway bronchial neural plasticity changes,which resulted in the airway hyperresponsiveness.