CAJ | 학술논문

目的制备幽门螺杆菌(Hp)菌蜕(BG)，并装载阿霉素，观察其对胃癌细胞的杀伤作用。方法通过细菌结合作用将裂解质粒导入Hp，42℃诱导细菌裂解制备Hp BG，经悬浮、离心装载阿霉素，分光光度法检测阿霉素装载量，四甲基偶氮唑蓝(MTT)法观察Hp BG-阿霉素对胃癌细胞SGC7901的杀伤作用。结果成功制备Hp BG并装载阿霉素，阿霉素的装载量为70.4μg/mg。共聚焦显微镜可见HpBG能被胃癌细胞吸附和内化，分布于SGC7901细胞表面或胞质，所携带的阿霉素传递入胃癌细胞，主要积聚于细胞核。MTT法检测显示SGC7901细胞对HpBG-阿霉素的IC50值为0.32±0.15，明显低于游离阿霉素（0.44±0.15，P＜0.05）。结论HpBG-阿霉素能有效抑制胃癌细胞生长，HpBG有望成为理想的抗胃癌药物载体。
목적 제비유문라간균(Hp)균세(BG)，병장재아매소，관찰기대위암세포적살상작용。방법통과세균결합작용장렬해질립도입Hp，42℃유도세균렬해제비Hp BG，경현부、리심장재아매소，분광광도법검측아매소장재량，사갑기우담서람(MTT)법관찰Hp BG-아매소대위암세포SGC7901적살상작용。결과 성공제비Hp BG병장재아매소，아매소적장재량위70.4μg/mg。공취초현미경가견HpBG능피위암세포흡부화내화，분포우SGC7901세포표면혹포질，소휴대적아매소전체입위암세포，주요적취우세포핵。MTT법검측현시SGC7901세포대HpBG-아매소적IC50치위0.32±0.15，명현저우유리아매소（0.44±0.15，P＜0.05）。결론HpBG-아매소능유효억제위암세포생장，HpBG유망성위이상적항위암약물재체。
Objective To synthesize H.pylori bacterial ghosts (BG) and loaded with adriamycin.The cytotoxic effects in gastric cancer cell line were also observed.Methods The lysis plasmid was introduced into H.pylori by bacterial conjugation. H.pylori BG were produced by inducing H.pylori lysis at 42 ℃.After suspension and centrifuge, H.pylori BG were loaded with adriamycin.The adriamycin loading quantity was measured with spectrophotometry.The cytotoxic effects of H.pylori BG-adriamycin in gastric cancer cell line SGC7901 were evaluated with MTT assay.Results H.pylori BG were successfully synthesized and loaded with adriamycin.The loading quantity of adriamycin was 70.4 μg/mg.H.pylori BG were seen to be adsorbed and internalized by gastric cancer cells under confocal microscope, which distributed on the surface or cytoplasmic of SGC7901 cell line. Carried Adriamycin was delivered into gastric cancer cell line and mainly accumulated in the nucleus.IC50 of SGC7901 to H.pylori BG-adriamycin was 0.32 ± 0.15 by MTT assay, which was significantly lower than that to free adriamycin (0.44 ±0.15, P＜0.05).Conclusions The proliferation of gastric cancer cells were effectively inhibited by H.pylori BG-adriamycin.H.pylori BG are expected to be ideal carrier for anti-gastric cancer medicine.