中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
3期
446-448
,共3页
杨成广%袁青领%于嵩%王志刚%郑起
楊成廣%袁青領%于嵩%王誌剛%鄭起
양성엄%원청령%우숭%왕지강%정기
ghrelin%平滑肌%肠神经丛%小肠转运
ghrelin%平滑肌%腸神經叢%小腸轉運
ghrelin%평활기%장신경총%소장전운
ghrelin%Smooth muscle%Enteric plexus%Small intestinal transit
目的 探讨ghrelin对大鼠小肠动力的外周作用机制.方法 观察不同浓度ghrelin(0、20、40、80 μg/kg)对大鼠小肠转运的影响,ghrelin(0.1、0.5、1.0μmol/L)对体外大鼠小肠平滑肌肌条收缩的影响,免疫荧光方法检测ghrelin受体(GHS-R1a)在小肠肌层中的分布.结果 ghrelin 能够剂量依赖性地增加小肠转运率(42.73±0.57)％、(47.13 ±0.84)％、(56.88±1.67)％、(69.04±1.79)％,增强卡巴胆碱引起的肌条收缩(152±3)％、(182±4)％、(218±3)％,ghrelin受体主要分布在肠内肌层中的神经细胞膜上.结论 ghrelin可通过作用于小肠肌层中的神经细胞而增强小肠平滑肌的收缩.
目的 探討ghrelin對大鼠小腸動力的外週作用機製.方法 觀察不同濃度ghrelin(0、20、40、80 μg/kg)對大鼠小腸轉運的影響,ghrelin(0.1、0.5、1.0μmol/L)對體外大鼠小腸平滑肌肌條收縮的影響,免疫熒光方法檢測ghrelin受體(GHS-R1a)在小腸肌層中的分佈.結果 ghrelin 能夠劑量依賴性地增加小腸轉運率(42.73±0.57)％、(47.13 ±0.84)％、(56.88±1.67)％、(69.04±1.79)％,增彊卡巴膽堿引起的肌條收縮(152±3)％、(182±4)％、(218±3)％,ghrelin受體主要分佈在腸內肌層中的神經細胞膜上.結論 ghrelin可通過作用于小腸肌層中的神經細胞而增彊小腸平滑肌的收縮.
목적 탐토ghrelin대대서소장동력적외주작용궤제.방법 관찰불동농도ghrelin(0、20、40、80 μg/kg)대대서소장전운적영향,ghrelin(0.1、0.5、1.0μmol/L)대체외대서소장평활기기조수축적영향,면역형광방법검측ghrelin수체(GHS-R1a)재소장기층중적분포.결과 ghrelin 능구제량의뢰성지증가소장전운솔(42.73±0.57)％、(47.13 ±0.84)％、(56.88±1.67)％、(69.04±1.79)％,증강잡파담감인기적기조수축(152±3)％、(182±4)％、(218±3)％,ghrelin수체주요분포재장내기층중적신경세포막상.결론 ghrelin가통과작용우소장기층중적신경세포이증강소장평활기적수축.
Objective To investigate the peripheral mechanism of ghrelin on the motility of rat small intestine.Methods The effect of different concentrations of ghrelin (0,20,40,80 μg/kg) on the small intestinal transit in vivo and ghrelin (0.1,0.5,1.0 μmol/L) on the contraction of smooth muscle strips of rat small intestine in vitro was observed.The locations of ghrelin receptors (GHS-R1a) were detected by using immunofluorescence.Results Ghrelin could dose-dependently increase the percentage of the small intestinal transit [ ( 42.73 ± 0.57 ) ％,(47.13 ± 0.84 ) ％,( 56.88 ± 1.67 ) ％,( 69.04 ±1.79)％ ] and enhance the contraction of smooth muscle strips mediated by carbachol [ (152 ± 3)％,(182 ± 4 )％,(218 ± 3 )％ ],and ghrelin receptors were mainly located on membrane of the nerve cells in the muscle layers.Conclusion Ghrelin could enhance the contraction of smooth muscle by activating the nerve cells in the muscle layers of small intestine.
