中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
2期
103-106
,共4页
仇金荣%唐奇%林红%王忠灿%李玉华%朱进
仇金榮%唐奇%林紅%王忠燦%李玉華%硃進
구금영%당기%림홍%왕충찬%리옥화%주진
胰腺肿瘤%基因表达%滋养层细胞表面抗原2
胰腺腫瘤%基因錶達%滋養層細胞錶麵抗原2
이선종류%기인표체%자양층세포표면항원2
Pancreatic neoplasms%Gene expression%Trop-2
目的 观察并探讨人滋养层细胞表面抗原2(Trop-2)在人胰腺癌中的表达及其临床意义.方法 用RT-PCR检测8例胰腺癌组织中Trop-2 mRNA的表达,免疫荧光检测人胰腺癌细胞系BxPc3中Trop-2蛋白的表达;免疫组化方法检测Trop-2蛋白在31例人胰腺癌组织及其癌旁组织芯片上的表达情况.统计分析Trop-2的表达与相应临床病理特征之间的关系.结果 胰腺癌组织中Trop-2 mRNA有表达,Trop-2蛋白主要表达在胰腺癌细胞膜上;Trop-2在人胰腺癌旁组织中的阳性表达率为9.7%,胰腺癌中阳性率为87.1%,其阳性表达程度随癌组织分化程度减低而增高(P＜0.01);Trop-2的表达与患者年龄、性别无关.结论 Trop-2的表达与人胰腺癌的发展及恶性程度有关,可作为临床检测胰腺癌恶性程度标记,也可作为靶向治疗胰腺癌的潜在靶点.
目的 觀察併探討人滋養層細胞錶麵抗原2(Trop-2)在人胰腺癌中的錶達及其臨床意義.方法 用RT-PCR檢測8例胰腺癌組織中Trop-2 mRNA的錶達,免疫熒光檢測人胰腺癌細胞繫BxPc3中Trop-2蛋白的錶達;免疫組化方法檢測Trop-2蛋白在31例人胰腺癌組織及其癌徬組織芯片上的錶達情況.統計分析Trop-2的錶達與相應臨床病理特徵之間的關繫.結果 胰腺癌組織中Trop-2 mRNA有錶達,Trop-2蛋白主要錶達在胰腺癌細胞膜上;Trop-2在人胰腺癌徬組織中的暘性錶達率為9.7%,胰腺癌中暘性率為87.1%,其暘性錶達程度隨癌組織分化程度減低而增高(P＜0.01);Trop-2的錶達與患者年齡、性彆無關.結論 Trop-2的錶達與人胰腺癌的髮展及噁性程度有關,可作為臨床檢測胰腺癌噁性程度標記,也可作為靶嚮治療胰腺癌的潛在靶點.
목적 관찰병탐토인자양층세포표면항원2(Trop-2)재인이선암중적표체급기림상의의.방법 용RT-PCR검측8례이선암조직중Trop-2 mRNA적표체,면역형광검측인이선암세포계BxPc3중Trop-2단백적표체;면역조화방법검측Trop-2단백재31례인이선암조직급기암방조직심편상적표체정황.통계분석Trop-2적표체여상응림상병리특정지간적관계.결과 이선암조직중Trop-2 mRNA유표체,Trop-2단백주요표체재이선암세포막상;Trop-2재인이선암방조직중적양성표체솔위9.7%,이선암중양성솔위87.1%,기양성표체정도수암조직분화정도감저이증고(P＜0.01);Trop-2적표체여환자년령、성별무관.결론 Trop-2적표체여인이선암적발전급악성정도유관,가작위림상검측이선암악성정도표기,야가작위파향치료이선암적잠재파점.
Objective To investigate the expression and its clinical significance of Trop-2 in human pancreatic cancer. Methods Reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescence assay were used to characterize the expression of Trop-2 in human pancreatic cancer.The expression of Trop-2 protein in 31 tumor tissue samples, including peritumoral tissue from patients with pancreatic cancer, was detected with immunohistochemisty by tissue microarray. The clinicopathological characteristics and the tissue expression of Trop-2 protein were analyzed statistically. Results RT-PCR,immunofluorescence assay and immunohistochemisty by tissue microarray showed a high expression of Trop-2 in pancreatic cancer. The expression rate of Trop-2 was much higher in pancreatic cancer than that in peritumoral tissues (87. 1% vs 9. 7% ). And a high expression of Trop-2 in pancreatic cancer was correlated with the low-differentiated changes. It had statistical significance ( P ＜ 0. 05 ). In contrast, no statistically significant correlation was found between the expression of Trop-2 and gender or age. Conclusion The expression of Trop-2 is correlated with the development and malignancy of pancreatic cancer. As a clinical prognostic marker, Trop-2 may be a potential therapeutic target for pancreatic cancer.
